ATRAP Expression in Brown Adipose Tissue Does Not Influence the Development of Diet-Induced Metabolic Disorders in Mice

Ohki, Kohji; Wakui, Hiromichi; Azushima, Kengo; Uneda, Kazushi; Haku, Sona; Kobayashi, Ryu; Haruhara, Kotaro; Kinguchi, Sho; Matsuda, Miyuki; Ohsawa, Masato; Maeda, Akinobu; Minegishi, Shintaro; Ishigami, Tomoaki; Toya, Yoshiyuki; Yamashita, Akio; Umemura, Satoshi; Tamura, Kouichi

doi:10.3390/ijms18030676

Cited by 11 publications

(8 citation statements)

References 37 publications

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“…Furthermore, PVAT contains different phenotypes of adipocytes i.e., white, brown, and beige adipocytes; and phenotype variability or changes in adipocytes in PVAT have been suggested to lead to different pathological roles in vascular disorders [1]. To this point, interestingly, researchers reported that decreased PVAT ‘browning’ in thoracic aortas of aging spontaneously hypertensive rats (8 wks vs. 16 wks) was associated with attenuated vasodilation effects of PVAT [43], but ATRAP in brown adipose tissue does not affect the development of obesity-related metabolic disorders [44]. Future studies investigating the relationship between changes in adipocytes phenotype and PVAT effects would provide significant mechanistic insight in the SHRSP.ZF model.…”

Section: Discussionmentioning

confidence: 99%

Perivascular Adipose Tissue-Enhanced Vasodilation in Metabolic Syndrome Rats by Apelin and N-Acetyl–l-Cysteine-Sensitive Factor(s)

Kagota

Maruyama‐Fumoto

Iwata

et al. 2018

IJMS

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Perivascular adipose tissue (PVAT) can regulate vascular tone. In mesenteric arteries of SHRSP.Z-Leprfa/IzmDmcr rats (SHRSP.ZF) with metabolic syndrome, vascular dysfunction is compensated by PVAT-dependent mechanisms that disappear with increasing age. In this study, we investigated the mechanisms of the age-related changes and responsible factor(s) involved in the enhancing effects of mesenteric arterial PVAT in SHRSP.ZF. Acetylcholine- and sodium nitroprusside-induced relaxations of isolated arteries were greater with PVAT than without PVAT at 17 and 20 weeks of age (wks), and as expected, this enhancement by the presence of PVAT disappeared at 23 wks. PVAT mRNA levels of angiotensin II type 1 (AT1) receptor-associated protein was less and AT1 receptor was unchanged at 23 wks when compared to 20 wks. At 20 wks, the enhanced acetylcholine-induced relaxation by the presence of PVAT was inhibited by N-acetyl-l-cysteine (NAC). Acetylcholine-induced relaxation of arteries without PVAT was increased in the presence of exogenously added apelin. PVAT mRNA level of apelin was higher in SHRSP.ZF than in control Wistar-Kyoto rats, and the level was decreased with aging. These results suggest that AT1 receptor activation in PVAT, and changes in the regulation of apelin and a NAC-sensitive factor are related to the age-dependent deterioration of the vasodilation enhancing effects of mesenteric arterial PVAT in SHRSP.ZF.

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Section: Discussionmentioning

confidence: 99%

Perivascular Adipose Tissue-Enhanced Vasodilation in Metabolic Syndrome Rats by Apelin and N-Acetyl–l-Cysteine-Sensitive Factor(s)

Kagota

Maruyama‐Fumoto

Iwata

et al. 2018

IJMS

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“…DRAXIN was related to Akt, which could impact milk synthesis [55,56]. AGTRAP was reported to have a functional role in adipose metabolism [57].…”

Section: Discussionmentioning

confidence: 99%

Identification of SNPs and Candidate Genes for Milk Produce Ability in Yorkshire Pig

Shi

Liu

et al. 2021

Preprint

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Background: Sow milk produce ability is an important limiting factor impacting suboptimal growth and survival of piglets. By pig genetic improvement, the litter size has been increased, and larger litters need more suckled mammary glands, that results in increased milk from lactating sow. Hence, it has much significance to explore the sow lactation performance. The aim of this study was to estimate genetic parameters and screen single nucleotide polymorphisms (SNPs) for milk produce ability trait in 985 Yorkshire pigs by a genome-wide association study (GWAS), and to further identify the candidate genes.Results: By ASReml, we estimated the heritability of sow milk produce ability: 0.18 ± 0.07. With the Fixed and random effect model Circulating Probability Unification (FarmCPU), we performed a GWAS, and detected seven genome-wide significant SNPs, namely, Sus scrofa Chromosome (SSC) 2: ASGA0010040 (P = 7.73E-11); SSC2:MARC0029355 (P = 1.30E-08), SSC6: WU_10.2_6_65751151 (P = 1.32E-10), SSC7: MARC0058875 (P = 4.99E-09), SSC10: WU_10.2_10_49571394 (P = 6.79E-08), SSC11: M1GA0014659 (P = 1.19E-07), and SSC15: MARC0042106 (P = 1.16E-07). We performed the distribution of phenotypes corresponding to genotypes of the seven SNPs, and showed that ASGA0010040, MARC0029355, MARC0058875, WU_10.2_10_49571394, M1GA0014659, and MARC0042106 have extreme phenotypic values corresponded to the homozygous genotypes, while the intermediate values corresponded to the heterozygous genotypes. Further, we screened for flanking regions ± 200 kb nearby seven significant SNPs, and identified 30 genes. Among of them, 24 as the candidates were involved in lactose metabolism, colostrum immunity, milk protein, and milk fat by the gene ontology (GO) and kyoto encyclopedia of genes and genomes (KEGG) analysis. Through the combined analysis between 24 candidate genes and differently expressed genes (DEGs) detected in transcriptome data (GSE101983), we found 11 commons (NAV2, ANO3, MUC15, DISP3, FBXO6, CLCN6, SLA-DQB1, PSMB8, PSMB9, TAP1, and KIF5C). Further, by comparing the chromosome positions of the candidate genes with the quantitative trait locus (QTLs) previously reported, a total of 13 genes were found to be within 0.86 Mb to 93.92 Mb of the reported QTLs for sow milk yield, in which, NAV2 was found to be located with 0.86 Mb of the QTL region ssc2: 40936355.Conclusions: In conclusion, we identified seven significant SNPs located on SSC2, 6, 7, 10, 11, and 15, and proposed 24 candidate genes for milk produce ability trait in Yorkshire pig. Among of them, 11 were the key candidates. These results contribute to the identification of variants and candidate genes for sow milk produce ability.

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“…The GTT and ITT were performed as previously described 15 , 47 . Briefly, for the GTT, blood glucose concentrations were measured with a blood glucose test metre (Glutest Neo Super; Sanwa-Kagaku) using blood samples that were taken from the tail tip of overnight-fasted mice.…”

Section: Methodsmentioning

confidence: 99%

“…Total RNAs were extracted from WAT, interscapular BAT, and soleus muscle tissue with ISOGEN (Nippon Gene), and the cDNA was synthesized using the SuperScript III First-Strand System (Invitrogen). Real-time quantitative RT-PCR was performed with an ABI PRISM 7000 Sequence Detection System by incubating the reverse transcription product with TaqMan PCR Master Mix and a designed Taqman probe (Applied Biosystems), essentially as described previously 47 . The mRNA levels were normalized to those of the 18SrRNA or glyceraldehyde-3-phosphate dehydrogenase (GAPDH) control.…”

Section: Methodsmentioning

confidence: 99%

Angiotensin II Type 1 Receptor-associated Protein Inhibits Angiotensin II-induced Insulin Resistance with Suppression of Oxidative Stress in Skeletal Muscle Tissue

Ohki

Wakui

Kishio

et al. 2018

Sci Rep

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Enhancement of AT1 receptor-associated protein (ATRAP) in adipose tissue improves high fat diet (HFD)-induced visceral obesity and insulin resistance, and suppresses adipose oxidative stress. However, HFD loading is not a direct stimulatory factor for AT1 receptor. In the present study, we investigated the effect of chronic, low-dose angiotensin II (Ang II) stimulation on glucose and lipid metabolism in mice and functional role of ATRAP. ATRAP expression was higher in adipose tissue (5–10-fold) and skeletal muscle tissue (approximately 1.6-fold) in ATRAP transgenic (TG) mice compared with wild-type (WT) mice. After Ang II infusion, insulin sensitivity was impaired in WT mice, but this response was suppressed in TG mice. Unexpectedly, Ang II infusion did not affect the adipose tissue profile in WT or TG mice. However, in skeletal muscle tissue, Ang II stimulus caused an increase in oxidative stress and activation of p38 MAPK, resulting in a decrease in glucose transporter type 4 expression in WT mice. These responses were suppressed in TG mice. Our study suggests that Ang II-induced insulin resistance is suppressed by increased ATRAP expression in skeletal muscle tissue. Hyperactivity of AT1 receptor could be related to formation of insulin resistance related to metabolic syndrome.

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ATRAP Expression in Brown Adipose Tissue Does Not Influence the Development of Diet-Induced Metabolic Disorders in Mice

Cited by 11 publications

References 37 publications

Perivascular Adipose Tissue-Enhanced Vasodilation in Metabolic Syndrome Rats by Apelin and N-Acetyl–l-Cysteine-Sensitive Factor(s)

Perivascular Adipose Tissue-Enhanced Vasodilation in Metabolic Syndrome Rats by Apelin and N-Acetyl–l-Cysteine-Sensitive Factor(s)

Identification of SNPs and Candidate Genes for Milk Produce Ability in Yorkshire Pig

Angiotensin II Type 1 Receptor-associated Protein Inhibits Angiotensin II-induced Insulin Resistance with Suppression of Oxidative Stress in Skeletal Muscle Tissue

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