Atroposelective antibodies as a designed protein scaffold for artificial metalloenzymes

Adachi, T.; Harada, Akira; Yamaguchi, Hiroyasu

doi:10.1038/s41598-019-49844-0

Cited by 4 publications

(2 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For the Friedel–Crafts reaction, Roelfes and coworkers previously developed LmrR-based ArMs [ 63 , 64 , 65 ]. Recently, Harada and coworkers employed monoclonal antibodies (mAbs) as protein scaffolds for ArMs catalyzing Friedel–Crafts reactions [ 66 ]. They previously developed atroposelective mAbs by immunological optimization of mAbs over binaphthyl (BN) derivatives [ 67 , 68 ].…”

Section: Recent Progress On the Various Reactions Catalyzed By Armmentioning

confidence: 99%

Artificial Metalloenzymes: From Selective Chemical Transformations to Biochemical Applications

Himiyama

Okamoto

2020

Molecules

View full text Add to dashboard Cite

Artificial metalloenzymes (ArMs) comprise a synthetic metal complex in a protein scaffold. ArMs display performances combining those of both homogeneous catalysts and biocatalysts. Specifically, ArMs selectively catalyze non-natural reactions and reactions inspired by nature in water under mild conditions. In the past few years, the construction of ArMs that possess a genetically incorporated unnatural amino acid and the directed evolution of ArMs have become of great interest in the field. Additionally, biochemical applications of ArMs have steadily increased, owing to the fact that compartmentalization within a protein scaffold allows the synthetic metal complex to remain functional in a sea of inactivating biomolecules. In this review, we present updates on: (1) the newly reported ArMs, according to their type of reaction, and (2) the unique biochemical applications of ArMs, including chemoenzymatic cascades and intracellular/in vivo catalysis. We believe that ArMs have great potential as catalysts for organic synthesis and as chemical biology tools for pharmaceutical applications.

show abstract

Section: Recent Progress On the Various Reactions Catalyzed By Armmentioning

confidence: 99%

Artificial Metalloenzymes: From Selective Chemical Transformations to Biochemical Applications

Himiyama

Okamoto

2020

Molecules

View full text Add to dashboard Cite

show abstract

“…[16][17][18][19][20][21][22][23][24][25][26][27][28][29] This intriguing feature allows asymmetric environmental fields to be constructed as the second coordination sphere for transition metal complexes, enabling a stereospecific reaction. 30,31 However, to the best of our knowledge, a hybrid catalyst consisting of a Pd-complex and a mAb is yet to be reported because typical Pd-complexes are unstable in water and degrade during immunization. Currently, there is only one report on the complexation of a Pd-complex of a porphyrin.…”

mentioning

confidence: 99%