Attachment of Vibrio cholerae serogroup O1 to zooplankton and phytoplankton of Bangladesh waters

Tamplin, Mark L.; Gauzens, Anne L.; Huq, Anwar; Sack, David A.; Colwell, Rita R.

doi:10.1128/aem.56.6.1977-1980.1990

Cited by 314 publications

(140 citation statements)

References 25 publications

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“…By culture methods none of the samples were positive, a result that was not surprising since V. cholerae isolation requires that samples be subjected to enrichment in alkaline peptone water prior to plating onto TCBS agar. Therefore, all samples were also tested by DFA (see Section 2); this technique was previously shown to be very sensitive in detecting both culturable and VBNC V. cholerae O1 in the aquatic environment [13,20,22]. In this case too, none of the examined samples were positive, a result not unexpected since V. cholerae are present in low numbers (ca.…”

Section: Resultsmentioning

confidence: 96%

Vibrios associated with plankton in a coastal zone of the Adriatic Sea (Italy)

Montanari¹,

Pruzzo²,

Pane³

et al. 1999

FEMS Microbiology Ecology

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From April 1996 through March 1997, culturable vibrios associated with both large (>200 μm) and small (0.45–200 μm) size plankton collected in the Ancona, Italy, coastal zone of the Adriatic Sea were studied. Vibrio alginolyticus was readily cultured from large size plankton from July to September and represented the only Vibrio species that could be cultured under the conditions employed in the study. Crustaceans (mainly copepods) and tunicates (Oikopleura spp.) comprised the larger sized plankton, with copepods being 90% of the total plankton population in the summer months. Diatoms (Chaetoceros spp.) were also present but in lower numbers. Vibrios associated with smaller sized plankton comprised mainly V. alginolyticus during June–September and in March, whereas Vibrio vulnificus was isolated from samples collected in April, May, and March. The smaller size plankton comprised predominantly tintinnids, nauplii of crustaceans and exuvia of copepods. These results confirm the role of zooplankton as hosts of vibrios in the environment, and suggest that smaller sized plankton may serve as an important reservoir of these bacteria.

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Section: Resultsmentioning

confidence: 96%

Vibrios associated with plankton in a coastal zone of the Adriatic Sea (Italy)

Montanari¹,

Pruzzo²,

Pane³

et al. 1999

FEMS Microbiology Ecology

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“…Mourino-Perez et al, 2003;Thompson et al, 2004b;Hsieh et al, 2007). Several studies have shown that Vibrio spp., in particular V. cholerae, have the ability to colonize particles such as zooplankton and phytoplankton (Huq et al, 1983;Tamplin et al, 1990;Montanari et al, 1999). Potentially, eutrophication caused by nutrient loading could have a small but significant impact on Vibrio spp.…”

Section: Discussionmentioning

confidence: 99%

Dynamics and predictive modelling of Vibrio spp. in the Neuse River Estuary, North Carolina, USA

Hsieh

Fries

Noble³

2007

Environmental Microbiology

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Vibrio spp. are naturally occurring bacteria in marine and estuarine environments around the world. The genus includes several human and animal pathogens that can negatively impact human health, seafood and aquaculture. Vibrio spp. populations are capable of rapid adaptation in response to changing environmental conditions, making them dynamic over short-term and seasonal scales. Temperature, vertical mixing, tidal flushing, climate, precipitation and nutrient loading can change the estuarine environment and subsequently alter microbial community structure, including Vibrio spp., affecting estuarine water quality and public health. To describe these dynamics, Vibrio spp. concentrations and a range of microbial, physical and chemical measures were monitored every 2 weeks and after storm events for 19 months in the Neuse River Estuary (NRE). Results showed clear seasonal and geographic trends in Vibrio spp. abundance. Multiple regression analysis revealed a strong relationship to temperature and salinity, with additional minor influences of chlorophyll a and dissolved organic carbon. Similar models based on easily measured environmental parameters should be pursued for individual Vibrio species in the NRE and other estuarine environments. Predictive models provide useful information for managers, researchers and modellers of estuarine ecosystems.

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“…However, most V. cholerae strains grew on NAG and/or diatom chitin. The distribution and survival of V. cholerae in marine ecosystems have been of great interest for decades [43] and attachment to surfaces of zooplankton, in particular the copepods, is an important reservoir of V. cholerae in the environment [44]. Surface membrane proteins in V. cholerae able to bind chitin particles were recently identi¢ed [45].…”

Section: Discussionmentioning

confidence: 99%

Use of a chiA probe for detection of chitinase genes in bacteria from the Chesapeake Bay

Ramaiah¹

2000

FEMS Microbiology Ecology

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PCR primers specific for the chiA gene were designed by alignment and selection of highly conserved regions of chiA sequences from Serratia marcescens, Alteromonas sp., Bacillus circulans and Aeromonas caviae. These primers were used to amplify a 225 bp fragment of the chiA gene from Vibrio harveyi to produce a chiA gene probe. The chiA PCR primers and probe were used to detect the presence of the chiA gene in an assemblage of 53 reference strains and gave consistent results. Selected chiA fragments amplified by PCR were cloned and sequenced from nine known strains and from Chesapeake Bay isolates 6d and 11d. This confirmed the specificity and utility of the primers for detection of chiA-positive environmental strains. Over 1000 bacterial isolates from Chesapeake Bay water samples were tested for the presence of the chiA gene which was found to be present in 5^41% (average 21%) of the culturable bacterial community. The approach developed in this study was valuable for isolation and enumeration of chiA-positive bacteria in environmental samples. ß

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Attachment of Vibrio cholerae serogroup O1 to zooplankton and phytoplankton of Bangladesh waters

Cited by 314 publications

References 25 publications

Vibrios associated with plankton in a coastal zone of the Adriatic Sea (Italy)

Vibrios associated with plankton in a coastal zone of the Adriatic Sea (Italy)

Dynamics and predictive modelling of Vibrio spp. in the Neuse River Estuary, North Carolina, USA

Use of a chiA probe for detection of chitinase genes in bacteria from the Chesapeake Bay

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