Attempted Type Specific Diagnosis of Dengue Virus Infection by the Indirect Fluorescent Antibody Method Directed at Differentiating IgM and IgG Responses

Measurement of Dengue Virus-Specific Double-Stranded Ribonucleic Acid in Infected Primate Cell Extracts by Microquantitative Complement-Fixation Methods

“…LLC-MK2 cells, a line of continuous rhesus monkey kidney, and Vero cells (analogous to LLC-MK2, but from a Cercopitliecus monkey) were grown and maintained as described [2,15]. The human lymphoblastoid cell line, PGLC-33H, was obtained from Dr. P hilip G lade and was maintained in suspension culture as described by him [4].…”

Dengue virus non-structural protein NS1: Structure, intracellular trafficking and role as a diagnostic marker

“…Detection of DENV by virus isolation is a definitive diagnostic test, but not particularly practical as isolation can take days to weeks to perform (Lanciotti et al, 1992 (Bundo and Igarashi, 1985;Burke et al, 1982;Cardosa et al, 1988;Clarke and Casals, 1958;Kuno et al, 1990;Russell et al, 1967;Vathanophas et al, 1973). The HI, IgM and IgG antibody capture ELISAs have proven to be the most used serological diagnostic methods for routine DENV infection.…”