Attempts at Culturing Mycobacteria from Tissues of Patients with Crohn’s Disease

Kreuzpaintner, G.; R, Kölble; Wallner, A.; Hesterberg, R.; Thorel, Marie-Françoise; Thomas, Lutz; Strohmeyer, G

doi:10.1007/978-94-011-1830-9_3

Cited by 2 publications

(4 citation statements)

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“…These were summarized as 10 isolates from a total of 180 patients (36). However, 2 of the 10 isolates were recorded only in abstract or table form, and lacked peerreviewed full description.…”

Section: Bacteriologic Culturementioning

confidence: 99%

“…Since 1982, when we first reported the recovery of a mycobacterium that was later identified as M. paratuberculosis from the tissues of two patients with CD (35), there have been only seven or eight similar recoveries. These were summarized as 10 isolates from a total of 180 patients (36). However, 2 of the 10 isolates were recorded only in abstract or table form, and lacked peerreviewed full description.…”

Section: Bacteriologic Culturementioning

confidence: 99%

“…Those who support the hypothesis that M. paratuberculosis is responsible for CD reconcile their failures at classic recovery by culture by reporting that they often find small translucent colonies of 0.5 mm size, on the Herrold's egg yolk media, after many months of incubation, which when stained by an acid-fast method contain what are described as coccoid bodies and other amorphous material, pleomorphic forms and rods, or spheroplast-like agents (36)(37)(38)(39)(40). Some of these, one-third it is claimed, when tested by PCR for IS 900 were identified as M. paratuberculosis (41,42).…”

Section: Bacteriologic Culturementioning

confidence: 99%

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Lack of support for a common etiology in Johne's disease of animals and Crohn's disease in humans

Kruiningen

2007

Inflamm Bowel Dis

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Summary:The superficial similarity of Johne's disease to Crohn's disease led to the hypothesis that, like the former, Crohn's disease was caused by Mycobacterium paratuberculosis. Detailed pathologic comparisons, however, reveal little similarity between these two entities, including the lack of important extraintestinal manifestations. Attempts to recover M. paratuberculosis by culture have only rarely succeeded and the significance of spheroplasts that appear more frequently on culture is seriously in question. Five immunocytochemistry studies have failed to find mycobacterial antigens in diseased tissues and the five most recent polymerase chain reaction (PCR) attempts to find genomic evidence of M. paratuberculosis were uniformly negative. Numerous serologic studies failed to demonstrate antibody to M. paratuberculosis and attempts to show cell-mediated immunity were also unrewarding. Inoculation of numerous experimental animals with Crohn's disease tissue has failed to induce Johne's disease, and inoculation of various animal species with M. paratuberculosis has equally failed to result in Crohn's disease. Controlled studies of the treatment of Crohn's disease with antimycobacterial agents have generally resulted in no improvement, and most studies that have shown a positive response are either uncontrolled or include broad-spectrum antibiotics that may be acting on pathogens other than mycobacteria. Finally, although Johne's disease is common in farm animals, and infected animals shed M. paratuberculosis in large numbers, no record of zoonotic transmission has been recorded. Key Words: Crohn's diseaseMycobacteria-Johne's disease-Zoonoses-Spheroplasts.Among research scientists, public health veterinarians, and the milk-consuming public there continues to be concern about the possibility that Mycobacterium paratuberculosis, the causative agent of Johne's disease (JD) of ruminants, is transmissible to humans and that it causes Crohn's disease (CD). Since the hypothesis that M. paratuberculosis causes CD was first mooted, much evidence that argues against it has accumulated. The purpose of this article is to summarize this evidence and to review the relationship between JD of animals and CD. PATHOLOGYA statement that is reiterated often is that which claims that CD is remarkably similar to JD. This author contends that the morphologic similarities are superficial, and in light of current histopathologic interpretation, more suggestive of differing etiopathogenesis.Address correspondence and reprint requests Lo Dr. H. J. Van Kruiningen, Department of Pathobiology, University of Connecticut, 61 N. Eagleville Rd., U-89, Storrs, CT 06269-3089, U.S.A.Manuscript received November 19, 1998; accepted March 24, 1999. Crohn's disease is a chronic, segmental disease of the intestine; it is submucosal and transmural, fibrosing, sclerosing, and stenosing (1-6). Affected most frequently are the distal ileum and proximal colon; however, CD sometimes occurs in other portions of the gastrointestinal tract. Segmental disea...

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Section: Bacteriologic Culturementioning

confidence: 99%

Section: Bacteriologic Culturementioning

confidence: 99%

Section: Bacteriologic Culturementioning

confidence: 99%

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Lack of support for a common etiology in Johne's disease of animals and Crohn's disease in humans

Kruiningen

2007

Inflamm Bowel Dis

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“…5,6 It was said for a time, following investigations in several countries, that a total of 10 cultures had been recovered from 180 patients. 7,8 During those studies of the 80s, it was recognized that Van Patter had done his PhD research on the MPTB possibility, presented as a thesis in 1952. 9 After thoroughly training in an MPTB lab, he conducted 1762 culture attempts on tissues from 43 CD patients, with seven different selective media, and incubated them up to 15 months.…”

Section: Bacteriologic Culturementioning

confidence: 99%

Where are the weapons of mass destruction — the Mycobacterium paratuberculosis in Crohn's disease?

Kruiningen¹

2011

Journal of Crohn's and Colitis

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Attempts at Culturing Mycobacteria from Tissues of Patients with Crohn’s Disease

Cited by 2 publications

References 20 publications

Lack of support for a common etiology in Johne's disease of animals and Crohn's disease in humans

Lack of support for a common etiology in Johne's disease of animals and Crohn's disease in humans

Where are the weapons of mass destruction — the Mycobacterium paratuberculosis in Crohn's disease?

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