Attempts at producing a hybridised Penaeus mondon cell line by cellular fusion

Claydon, Kerry; Roper, Katrina; Owens, Leigh

doi:10.1016/j.fsi.2010.05.012

Cited by 9 publications

(9 citation statements)

References 32 publications

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“…However, this also did not lead to immortalization of cell cultures. The lack of success in spontaneous and induced cell line development subsequently paved the way for the attempts on developing fusion cell line [15,16], but, with little success. More recently, researchers have succeeded in viral gene expression [42], determinations of cytotoxicity and genotoxicity [41,43], and viral multiplication [26] employing primary cell culture systems developed from different species of penaeids.…”

Section: History Of Shrimp Cell Culturementioning

confidence: 99%

“…As spontaneous and induced transformation of somatic penaeid cells has not taken place [15], attempts to create hybrid cells by fusing cells from an immortal cell line of insects (Epithelioma papulosum cyprinid and Spodoptera frugiperda) with haemocytes from P. monodon were made and accordingly three fusion-cells could be produced (F11, F12 and F13). However, shrimp genes and viral susceptibility could not be observed in the fusion-cells; this happens to be the first attempt to produce hybrid cells from shrimp cells.…”

Section: Molecular Approaches For Shrimp Cell Immortalizationmentioning

confidence: 99%

“…Till date, no permanent cell line could be made available from marine invertebrates in general [67] and shrimp in particular. The major fall out of the situation is the impediment which it imposes on the isolation of crustacean viruses [15,16,43]. The fact is that the requirement of continuous cell lines is so high to investigate the radiating viral threats to shrimp aquaculture [21,88,93].…”

Section: Introductionmentioning

confidence: 99%

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Establishment of Shrimp Cell Lines: Perception and Orientation

2012

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Development of continuous shrimp cell lines for effective investigation on shrimp viruses remains elusive with an arduous history of over 25 years. Despite presenting challenges to researchers in developing a cell line, the billion dollar aquaculture industry is under viral threat. Advances in molecular biology and various gene transfer technologies for immortalization of cells have resulted in the development of hundreds of cell lines from insects and mammals, but yet not a single cell line has been developed from shrimp and other marine invertebrates. Though improved growth and longevity of shrimp cells in vitro could be achieved by using modified growth media this did not make any leap to spontaneous transformation; probably due to the fact that shrimp cells inhibited neoplastic transformations. Oncogenic induction and immortalization are considered as the possible ways, and an exclusive medium for shrimp cell culture and an appropriate mode of transformation are crucial. In this review status of shrimp cell line development and its future orientation are discussed.

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Section: History Of Shrimp Cell Culturementioning

confidence: 99%

Section: Molecular Approaches For Shrimp Cell Immortalizationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Establishment of Shrimp Cell Lines: Perception and Orientation

2012

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“…Recently, Claydon et al (2010) have attempted developing a hybridized Penaeus monodon cell line by cellular fusion for testing virus pathogenicity but it is reported that such cells lack crustacean components required for the use as an in vitro system for virus replication. Therefore, this testicular cell line could be a very useful tool for testing infection and replication of crustacean viruses such as WSSV, hypodermal and haematopoietic necrosis viruses that cause damages to the aquaculture industry.…”

Section: Discussionmentioning

confidence: 99%

“…Although primary cell cultures from crustacea have been initiated since 1960s, no established cell line of marine crustacean is reported (Claydon and Owens 2008) except a transformed shrimp lymphoid cell line using pSV-3 neo vector containing the tumor antigen gene from Simian virus-40 (Tapay et al 1995) and hybridized Penaeus monodon cell line by cellular fusion (Claydon et al 2010). However, primary cell cultures from several crustacean species using various culture conditions have been reported with increased frequency.…”

Section: Introductionmentioning

confidence: 99%

Development of cell line from the testicular tissues of crab Scylla serrata

Shashikumar

Desai

2011

Cytotechnology

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This is the first report on development of a finite cell line from testicular tissues of crab, Scylla serrata. Both the explant and segregated tissues of testes yielded cells that could proliferate and grow. These cells ranged in size from 10 to 38 lm with distinct nuclei of varying shapes. The testicular cells survived and proliferated best in L-15-crab saline medium supplemented with epidermal growth factor (20 ng/mL) and glucose (1 mg/mL). The cell proliferation rate was assessed by Methyl tetrazolium assay in terms of change in optical density which clearly indicated a prominent increase in cell density. The testicular cells were subcultured at an interval of 4-6 days. These subcultured cells remained healthy and proliferated for 5 months with a minimum of ten subsequent passages. The finite cell line was characterized in terms of morphology, growth rate, lactate dehydrogenase release (for detecting health status) and 18S rRNA sequencing. This cell line could be a very useful tool for testing infections and replications of crustacean viruses. The present work provides a technique that could be extended for developing other crustacean cell lines.

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Misidentification of OLGA-PH-J/92, believed to be the only crustacean cell line

Lee

Bufalino

Christie

et al. 2011

In Vitro Cell.Dev.Biol.-Animal

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Continuous cell lines from aquatic invertebrate species are few and the development of crustacean cell lines remains an elusive goal. Although a crayfish cell line derived from neural ganglia of Orconectes limosus was reported in 2000, this cell line OLGA-PH-J/92 failed to be authenticated as such. In this report, we describe our attempts to identify the taxonomic identity of the cell line through immunological and molecular techniques. Immunohistochemical screening for the expression of a suite of invertebrate neuropeptides gave negative results, precluding an invertebrate neural origin. PCR amplification and DNA sequencing for the mitochondrial cytochrome c oxydase I, and 18S ribosomal RNA genes that had been widely used to confirm species identity, could not confirm the OLGA-PH-J/92 cells as originating from crayfish. Subsequent attempts to identify the cells provided moderate homology (82%) to Gephyramoeba sp. (AF293897) following PCR amplification of an 18S rDNA fragment after a BLAST search. A literature search provided morphological evidence of the similarity of OLGA-PH-J/92 to the Gephyramoeba distributed by the American Type Culture Collection as ATCC 50654, which also had been misidentified and was renamed Acramoeba dendroida (Smirnov et al., Eur J Protistol 44:35-44, 2008). The morphology of the OLGA-PH-J/92 cells which remains identical to the original report (Neumann et al., In Vivo 14:691-698, 2000) and matched corresponding micrographs that were available from the ATCC before the cell line was dropped from their catalog (ATCC CRL 1494) is very similar to A. dendroida and could thus belong to the Acramoebidae. These results unequivocally indicate that the OLGA-PH-J/92 cell line is not derived from the crayfish O. limosus, and the search for an immortal crustacean cell line continues.

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Attempts at producing a hybridised Penaeus mondon cell line by cellular fusion

Cited by 9 publications

References 32 publications

Establishment of Shrimp Cell Lines: Perception and Orientation

Establishment of Shrimp Cell Lines: Perception and Orientation

Development of cell line from the testicular tissues of crab Scylla serrata

Misidentification of OLGA-PH-J/92, believed to be the only crustacean cell line

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