Attenuated total reflectance infrared studies of liposome adsorption at the solid–liquid interface

Er, Yan; Prestidge, Clive A.; Fornasiero, Daniel

doi:10.1016/j.colsurfb.2004.06.002

Cited by 16 publications

(13 citation statements)

References 36 publications

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“…Phospholipid adsorption onto solids yields characteristic peaks of alkyl chains and phosphate groups which are present in the hydrophobic tails and head groups, respectively. [85][86][87][88] Figure 2b shows IRRAS spectra obtained for a-C (left) and a-C:O (right) surfaces in the C-H stretching region after immersion in suspensions over the 4.5-9.5 pH range; all spectra are ratioed against a pristine a-C background.…”

Section: Resultsmentioning

confidence: 99%

“…a-C and a-C:O surfaces were incubated in phosphate buffered liposome suspensions for 1 h, rinsed in water, dried, and subsequently analyzed by IRRAS. Phospholipid adsorption onto solids yields characteristic peaks of alkyl chains and phosphate groups that are present in the hydrophobic tails and head groups, respectively. − Figure b shows IRRAS spectra obtained for a-C (left) and a-C:O (right) surfaces in the C–H stretching region after immersion in suspensions over the 4.5–9.5 pH range; all spectra are ratioed against a pristine a-C background. Peaks at 2924 and 2853 cm –1 are assigned to −CH 2 antisymmetric and symmetric stretching modes, while the shoulder at 2965 cm –1 is assigned to −CH 3 stretching modes. , IRRAS spectra of a-C surfaces display strong absorbances associated with the phospholipid alkyl tails at all pH values examined; however, in the case of a-C:O, it was not possible to detect any peaks independently of pH.…”

Section: Resultsmentioning

confidence: 99%

“…Several studies have reported on the interaction of lipid aggregates with model solid surfaces, such as SiO 2 , TiO 2 , and mica, and showed that wettability is critical in determining the outcome of liposome adsorption at the solid/liquid interface. , It has been observed that at hydrophobic surfaces liposomes adsorb preferentially as supported vesicle layers (SVL) or supported lipid monolayers (SLM), whereas at hydrophilic surfaces, a supported lipid bilayer (SLB) can form. This behavior was reviewed by Tero et al who attributed the liposome structure at hydrophilic surfaces to the higher density of −OH groups, which correlates to the increase of hydration repulsion forces between the stable layer of water molecules attached to the phospholipid head groups and the water bound to the solid substrate.…”

Section: Discussionmentioning

confidence: 99%

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Understanding the Carbon–Bio Interface: Influence of Surface Chemistry and Buffer Composition on the Adsorption of Phospholipid Liposomes at Carbon Surfaces

Vasconcelos

Zen

Angione

et al. 2020

ACS Appl. Bio Mater.

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Surface active phospholipids are present in fluids of biological relevance and their adsorption may condition and determine the response of carbon and nanocarbon surfaces when they are immersed in physiological media. In this work, the adsorption and assembly of liposomes at carbon interfaces was investigated to understand the effect of surface termination on the extent and mode of assembly of lipid aggregates. Liposomes of natural lipids were prepared from a mixture of phosphatidylcholine (PC) and phosphatidylserine (PS) and their hydrodynamic size and surface zeta potential were studied as a function of pH. Adsorption was investigated at graphitic amorphous carbon surfaces (a-C) and at these surfaces after oxidative treatments (a-C:O). Infrared surface spectroscopy experiments show that PC/PS liposomes adsorb at a-C surfaces exclusively, independently of pH, while no adsorption is observed at a-C:O materials.Nanogravimetry and fluorescence imaging experiments in solution indicate that adsorption at a-C occurs as supported intact vesicles. Interestingly, PC/PS adsorption at oxidized surfaces was observed only in the presence of a dication such as Ca +2 , a behaviour that was attributed to screening of surface-liposome repulsive electrostatic interactions. Vesicle rupture experiments show that lipids adsorb as monolayers on graphitic surfaces, whereas adsorbate structures correspond to bilayers in the case of oxidized carbons. These results therefore demonstrate a strong dependence of adsorbate structure on both carbon chemistry and buffer composition. These findings have important implications for the design of carbon nanoparticles, carbon electrodes or carbon coatings for applications in biology and medicine.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Understanding the Carbon–Bio Interface: Influence of Surface Chemistry and Buffer Composition on the Adsorption of Phospholipid Liposomes at Carbon Surfaces

Vasconcelos

Zen

Angione

et al. 2020

ACS Appl. Bio Mater.

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“…There were also wagging and rocking vibrations of -CH 2 groups, δ-vibrations at 1470 cm -1 and γ-vibrations at 1300 and 720 cm -1 . [29][30][31][32][33] The FTIR spectra of liposomes with incorporated β-carotene ( Fig. 2) and liposomes with lutein ( Fig.…”

Section: Resultsmentioning

confidence: 99%

Investigation into the potential chemical mechanism of the pro-oxidant activity of carotenoids with liposomes under UV-irradiation

Cvetkovic¹,

Stanojevic²,

Cakic³

et al. 2017

JSCS

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This study focuses on the behavior of ?-carotene and lutein inside multilamellar liposomes under continuous UV-irradiation. The liposomes were obtained by the thin film method and carotenoids (Crts) were incorporated by mixing at various concentrations (0.005, 0.0075, 0.02, 0.07 and 0.5 mol %). Liposomes formation and the presence of Crts inside them were confirmed by SEM microscopy and FT-IR spectroscopy, respectively. The antioxidant/pro- -oxidant activity of Crts inside liposomes was determined by the thiobarbituric acid?malondialdehyde (TBA?MDA) test. The investigated Crts acted more or less unexpected (as pro-oxidants) inside the lipid bilayers, interacting with the UV-produced lipid radicals and simultaneously suffering under the UV-irradiation. Their pro-oxidant activity with liposomes and under UV-irradiation could be explained by the formation of unstable adducts in the reaction with peroxyl radicals, or by Crts-cation radicals formation via the electron transfer mechanism. Such tentatively unexpected behavior of carotenoids should be taken into consideration in further carotenoids-based UV-filters projections in cosmetic formulations for skin protection. [Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. TR-34012]

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“…Tables II and III list the absorption wavelengths in ATR‐FTIR spectra of lithospermum and the lithospermum multiple phase emulsion cream 15–17. Figure 3 displays these ATR‐FTIR spectra.…”

Section: Resultsmentioning

confidence: 99%

Surface properties of lithospermum‐containing multiple phase emulsion systems

Tsai¹,

Lin²,

Wu³

et al. 2010

J of Applied Polymer Sci

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In this study, we employed a one-step emulsion process-rather than the traditional, relatively difficult two-step process-to obtain multiple phase emulsion systems containing the Chinese herbal medicine lithospermum. We characterized these systems in terms of their contact angles, surface tensions, fluorescence, and stability and analyzed them using polarized microscopy, atomic force microscopy, and attenuated total reflection Fourier transform infrared spectroscopy.Our one-step emulsion process involved mixing lithospermum at various concentrations with three different emulsifiers to control the properties of the emulsion systems.

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Attenuated total reflectance infrared studies of liposome adsorption at the solid–liquid interface

Cited by 16 publications

References 36 publications

Understanding the Carbon–Bio Interface: Influence of Surface Chemistry and Buffer Composition on the Adsorption of Phospholipid Liposomes at Carbon Surfaces

Understanding the Carbon–Bio Interface: Influence of Surface Chemistry and Buffer Composition on the Adsorption of Phospholipid Liposomes at Carbon Surfaces

Investigation into the potential chemical mechanism of the pro-oxidant activity of carotenoids with liposomes under UV-irradiation

Surface properties of lithospermum‐containing multiple phase emulsion systems

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