Attenuation of CCl4 Induced Oxidative Stress, Immunosuppressive, Hepatorenal Damage by Fucoidan in Rats

Abstract

The protective and therapeutic effects of fucoidan extract from Laminaria species against liver damage induced by CCl 4 in rats was investigated by monitoring the serum level and hepatic m-RNA expression of TGFβ-1, liver and renal markers, as well as oxidative stress and antioxidant biomarker. Thirty six adult male albino rats were divided into 4 equal groups; one was used as a negative control while groups II, III, and IV administrated 0.1 mL/100 g body weight twice a week for 8 weeks with carbon tetrachloride (CCl 4 ), fucoidan (400 mg/kgbw orally/day), and CCl 4 plus fucoidan, respectively. Blood samples were collected at the end of experiment and sera were separated to evaluate serum levels and the hepatic m-RNA expression of transforming growth factor beta (TGFβ-1), tumor necrosis factor (TNF α), interferon gamma (IFN-γ.), interleukin (IL), Il-1β, IL-6 and IL-10, antioxidant markers, reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and lipid peroxidation malondialdehyde (MDA) as well as selective biochemical markers of liver and kidney functions were estimated. The results of this investigation revealed that treatment with fucoidan improved elevated expression of liver TGF β-1, Il-1β, IL-6, TNF α and serum level of malnoaldehyde (MDA), total bilirubin (T. Bil), induced by CCl 4 at 8th week post treatment. In addition to enhancing the antioxidant enzyme activities, GSH, GPx, CAT and SOD. Also, liver trransaminase (ALT, AST), alkaline phosphatase (ALP), reduced in fucoidan and CCl 4 treated group. These results show that crude fucoidan has potential immunomodulatory, antioxidant and hepatoprotective effects against the hepatic damage induced by CCl 4 .

Fucoidan Ameliorates Oxidative Stress, Inflammation, DNA Damage, and Hepatorenal Injuries in Diabetic Rats Intoxicated with Aflatoxin B1

“…Similarly, FUC improved the liver functions in carbon tetrachloride, microcystin, and diazinon-induced hepatorenal injuries in murine models [55][56][57] and lowered serum AST and ALT activities in hepatitis C virus-infected subjects [58]. These effects may be explained by FUC antioxidant activity as evidenced by alleviated lipid oxidation and enhancement of the antioxidant defense system in the liver and kidneys (Tables 2 and 3) and [14,55]. These results were in accordance with previous published investigations.…”

“…The glucose-lowering effect of FUC might be due to enhancement of insulin secretion by pancreatic cells, increasing glucose uptake, or reduction of basal lipolysis [54]. Similarly, FUC improved the liver functions in carbon tetrachloride, microcystin, and diazinon-induced hepatorenal injuries in murine models [55][56][57] and lowered serum AST and ALT activities in hepatitis C virus-infected subjects [58]. These effects may be explained by FUC antioxidant activity as evidenced by alleviated lipid oxidation and enhancement of the antioxidant defense system in the liver and kidneys (Tables 2 and 3) and [14,55].…”

Defensive role of Rosmarinus officinalis in carbon tetrachloride-induced nephrotoxicity and oxidative stress in rats

“…Results obtained shows highly significant decrease (P < 0.001) in antioxidant markers, GSH, CAT, SOD, and GPx; however, a highly significant increase (P < 0.001) was noticed in the levels of XOD and LPO in the CCl 4 -treated group when compared with the control rats. Depletion of endogenous enzymatic and nonenzymatic antioxidants in CCl 4 intoxicated group could be attributed to CCl 4 generated cellular ROS production and the subsequent depletion of the antioxidant cellular system (Li et al 2005;Gowri et al 2008;Muhammad et al 2009;Boshy et al 2017 andBellassoued et al 2018). On the other hand, treatment with EERO alone at 100 mg/kg and 250 mg/kg does not show any significant change in the level of these biomarkers as compared with the control group.…”

The Effects of Oral Administration of Pulegone in Carbon Tetrachloride-Induced Oxidative Stress in Wistar Rats

“…arbon tetrachloride, CCl4 is usually applied to induce the toxicity and hepatic fibrosis. The hepatotoxic effect of CCl4 could be attributed to its fast cleavage through cytochrome P450 in hepatocytes, that produces trichloromethyl radicals leading to lipid peroxidation and then to membrane injury [1].…”