Augmentation of tissue ATP level during digestion using preoxygenated perfluorocarbon results in high islet yield—new strategy for islet isolation

Tanioka, Yasuki; Tanaka, Tomohiro; Gotoh, Takeo; Kakinoki, Keitaro; Li, S.; Yoshikawa, Tomoyuki; Sakai, Tetsuya; Fujino, Y.; Suzuki, Yasuyuki; Kuroda, Yoshikazu

doi:10.1016/j.transproceed.2004.12.232

Cited by 6 publications

(7 citation statements)

References 8 publications

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“…At the end of the isolation procedure an anoxic level is reached resulting in severe depletion of islet ATP content. This can be efficiently counteracted by the supplementation of preoxygenated perfluorocarbon and the simultaneous administration of ATP precursors during pancreas dissociation [29]. Whether this recently published procedure is feasible for human islet isolation needs further evaluation .…”

Section: Sources Of Nutritional and Inflammatory Stressmentioning

confidence: 99%

Ischaemia is linked to inflammation and induction of angiogenesis in pancreatic islets

Linn

Schmitz

Hauck‐Schmalenberger

et al. 2006

Clinical and Experimental Immunology

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Summaryβ β β β -cell replacement is the only way to restore euglycaemia in patients with type-1 diabetes. Pancreatic tissue, processed for subsequent clinical islet transplantation, is exposed to ischaemia causing injury and death in a large number of islets before and after transplantation. In this review we summarize what is known on the sources of environmental stress for pancreatic islets, such as insufficient oxygen supply during pancreas procurement and in culture prior to intraportal transplantation, nutritional and oxygen deprivation during the isolation process, and the consequences of hyperglycaemia. An increasingly recognized role in the modulation of β β β β -cell function and these environmental stress factors plays the vascular network of the pancreatic islets. Islet revascularization by angiogenesis is relevant for the survival of the graft subsequent to transplantation. Potential strategies offered by therapeutic induction of revascularization to ameliorate the detrimental impact of these factors on the quality of islet transplants are discussed.

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Section: Sources Of Nutritional and Inflammatory Stressmentioning

confidence: 99%

Ischaemia is linked to inflammation and induction of angiogenesis in pancreatic islets

Linn

Schmitz

Hauck‐Schmalenberger

et al. 2006

Clinical and Experimental Immunology

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“…Consequently, cold ischemia during preservation and warm ischemia during procurement were reduced by TLM. Several trials have shown that tissue ATP levels of the pancreas form the most reliable and sensitive marker of oxygen status during TLM [41]. Adenosine in the UW solution is the main substrate for ATP production in the pancreas damaged by ischemia [27].…”

Section: Discussionmentioning

confidence: 99%

Oxygenated Static Preservation of Donation after Cardiac Death Liver Grafts Improves Hepatocyte Viability and Function

Murakami²,

Aoki³

et al. 2015

Eur Surg Res

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Background: Cell therapy, such as hepatocyte transplantation (HTx), is promising for the treatment of metabolic liver diseases or as a bridge to orthotopic liver transplantation in patients with fulminant liver failure. However, one of the limitations of this therapy is the shortage of donors. The present study aims to investigate whether the two-layer method (TLM) of cold preservation with oxygenation improves the viability and activity of hepatocytes from rat donation after cardiac death (DCD) donors compared with results obtained with the University of Wisconsin (UW) solution. Moreover, we evaluated the hepatocyte function after culture or transplantation into the spleen. Materials and Methods: We used male Sprague-Dawley rats for this study. The DCD model was induced by phrenotomy after injecting heparin. We assigned rats based on warm ischemia times of 15 and 30 min to groups S and L, respectively. Each group (n = 5) was then subdivided as follows: (1) group S: not preserved (S/N), preserved by TLM for 3 h (S/TLM3) and 12 h (S/TLM12), and in the UW solution for 3 h (S/UW3) and 12 h (S/UW12), and (2) group L: not preserved (L/N), preserved by TLM for 3 h (L/TLM3) and 12 h (L/TLM12), and in the UW solution for 3 h (L/UW3) and 12 h (L/UW12). The cell viability and function of isolated DCD hepatocytes were analyzed for culture or HTx into the spleen. Results: The viability and ATP levels of DCD hepatocytes significantly improved after TLM compared with the values after preservation in cold UW solution in group S/N (p < 0.059). The levels of albumin production and urea synthesis by hepatocytes after culture were significantly higher in groups S/TLM3 and S/TLM12 than in groups S/UW3 and S/UW12 (p < 0.05), respectively. Further, serum albumin levels after HTx were also markedly higher in groups S/TLM3 and S/TLM12 than in groups S/UW3 and S/UW12. The morphological features revealed that cultured and transplanted hepatocytes remained clearly viable and maintained an expression for specific hepatic function, such as the production of albumin and glycogen. Conclusion: This novel method of oxygenated cold preservation of DCD livers can expand the hepatocyte donor pool for HTx and establish a wider application of this developing technique.

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“…Although several solutions have been used for liver preservation, maintenance of specific hepatic functions during cold preservation of hepatocytes has never been adequately demonstrated [22]. Addition of a PFC to UW solution has been proposed for several years for cold preservation of the pancreas before islet transplantation [31][32][33][34][35][36]. The TLM has been shown to be beneficial for preserving other organs aside from the pancreas [45,46].…”

Section: Discussionmentioning

confidence: 99%

“…The TLM has been demonstrated to improve the function of the pancreas and islet cell after cold preservation over simple UW cold storage in pancreas transplantation [23][24][25][26][27][28][29][30] and in islet transplantation [31][32][33][34][35][36]. The TLM has been demonstrated to directly oxygenate the pancreas through the PFC, and adenosine triphosphate (ATP) is continuously generated in the graft during preservation [35]. The ATP generated is used to drive a sodiumpotassium pump that maintains cellular integrity and parenchymal and nonparenchymal viability [37][38][39].…”

Section: Introductionmentioning

confidence: 99%

Cold Preservation of the Liver With Oxygenation by a Two-Layer Method

Odaira

Aoki

Miyamoto

et al. 2009

Journal of Surgical Research

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Augmentation of tissue ATP level during digestion using preoxygenated perfluorocarbon results in high islet yield—new strategy for islet isolation

Cited by 6 publications

References 8 publications

Ischaemia is linked to inflammation and induction of angiogenesis in pancreatic islets

Ischaemia is linked to inflammation and induction of angiogenesis in pancreatic islets

Oxygenated Static Preservation of Donation after Cardiac Death Liver Grafts Improves Hepatocyte Viability and Function

Cold Preservation of the Liver With Oxygenation by a Two-Layer Method

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