Augmented migration of mesenchymal stem cells correlates with the subsidiary CXCR4 variant

Heirani‐Tabasi, Asieh; Naderi‐Meshkin, Hojjat; Matin, Maryam Moghaddam; Mirahmadi, Mahdi; Shahriyari, Mina; Ahmadiankia, Naghmeh; Moussavi, Nasser Sanjar; Bidkhori, Hamid Reza; Raeesolmohaddeseen, Mahmood; Bahrami, Ahmad Reza

doi:10.1080/19336918.2016.1243643

Cited by 10 publications

(7 citation statements)

References 41 publications

(43 reference statements)

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“…In another study on a mesenchymal stem cell sheet, secretions of VEGF, bFGF and collagen I and III were enhanced under hypoxic conditions [ 39 ]. Along with an enhanced proliferation, the chemokine receptor (CXCR4) has also been demonstrated to be elevated on DPSCs under hypoxia [ 40 ]. The increased secretion of these pro-angiogenic and anti-inflammatory factors is what could provide a potential therapeutic application to DPSCs.…”

Section: Discussionmentioning

confidence: 99%

Modulation of the Dental Pulp Stem Cell Secretory Profile by Hypoxia Induction Using Cobalt Chloride

Bhandi

Kahtani

Mashyakhy

et al. 2021

JPM

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The action of stem cells is mediated by their paracrine secretions which comprise the secretory profile. Various approaches can be used to modify the secretory profile of stem cells. Creating a hypoxic environment is one method. The present study aims to demonstrate the influence of CoCl2 in generating hypoxic conditions in a dental pulp stem cell (DPSCs) culture, and the effect of this environment on their secretory profile. DPSCs that were isolated from human permanent teeth were characterized and treated with different concentrations of CoCl2 to assess their viability by an 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and proliferation by a cell counting kit (CCK)-8 assay. The gene expression level of hypoxia-inducible factor 1-alpha (HIF-1α) was analyzed by quantitative real time polymerase chain reaction (qRT-PCR) to demonstrate a hypoxic environment. Comparative evaluation of the growth factors and cytokines were done by cytometric bead array. Gene expression levels of transcription factors OCT4 and SOX2 were analyzed by qRT-PCR to understand the effect of CoCl2 on stemness in DPSCs. DPSCs were positive for MSC-specific markers. Doses of CoCl2, up to 20 µM, did not negatively affect cell viability; in low doses (5 µM), it promoted cell survival. Treatment with 10 µM of CoCl2 significantly augmented the genetic expression of HIF-1α. Cells treated with 10 µM of CoCl2 showed changes in the levels of growth factors and cytokines produced. It was very evident that CoCl2 also increased the expression of OCT4 and SOX2, which is the modulation of stemness of DPSCs. A CoCl2 treatment-induced hypoxic environment modulates the secretory profile of DPSCs.

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Section: Discussionmentioning

confidence: 99%

Modulation of the Dental Pulp Stem Cell Secretory Profile by Hypoxia Induction Using Cobalt Chloride

Bhandi

Kahtani

Mashyakhy

et al. 2021

JPM

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“…The most important role of Hypoxia-mimicking agents is considered as hypoxia-inducible factor-1a (HIF-1a) inducer, which becomes a critical regulator of the cellular reaction to hypoxia condition (Mirahmadi et al 2016b;Oses et al 2017;Woo et al 2006;Yoo et al 2016;). It was exhibited previously that in MSCs, hypoxia-mimicking agents boost stem cell migration by CXCR4/SDF-1 axis (Heirani-Tabasi et al 2018;Hung et al 2007;Tsai et al 2010). We hypothesized that these components may also prime Ad-MSCs for enhancement of homing by increasing migration-related signaling molecules.…”

Section: Introductionmentioning

confidence: 88%

Comparison the effects of hypoxia-mimicking agents on migration-related signaling pathways in mesenchymal stem cells

et al. 2020

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Adipose-derived mesenchymal stem cells (Ad-MSCs) have been designated as the promising agents for clinical applications for easy accessibility, multi-linage differentiation and immunomodulation capacity. Despite this, optimal cell delivery conditions have remained as a clinical challenge and improvement of stem cell homing to the target organs is being considered as a major strategy in cell therapy systemic injection. It has been shown that homing of mesenchymal stem cells are increased when treated with physical or chemical hypoxia-mimicking factors, however, efficiency of different agents remained to be determined. In this study, hypoxia-mimicking agents, including valproic acid (VPA), cobalt chloride (CoCl 2) and deferoxamine (DFX) were examined to determine whether they are able to activate signaling molecules involved in migration of Ad-MSCs in vitro. We report that Ad-MSCs treated by DFX resulted in a significantly enhanced mRNA expression of MAPK4 (associated with MAPK signaling pathway), INPP4B (associated with Inositol polyphosphate pathway), VEGF-A and VEGF-C (associated with cytokinecytokine receptor pathways), IL-8 and its receptor, CXCR2 (associated with IL-8 signaling pathway). While the cells treated with VPA did not show such effects and CoCl 2 only upregulated VEGF-A and VEGF-C gene expression. Furthermore, results of wound-healing assays showed migration capacity of Ad-MSCs treated with DFX significantly increased 8 and 24 h of the treatment. This study provides credible evidence around DFX, which might be an effective drug for pharmacological preconditioning of Ad-MSCs to boost their homing capacity and regeneration of damaged tissues though, activation of the migration-related signaling pathways.

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“…Additionally, CXCR4 signaling increased in a dose-dependent manner, which seemed to be the result of increased hypermethylation of the CXCR4 gene promoter and chromatin remodeling. These results showed that VPA (2.5 mM for 3 months) might be involved in the differentiation of MSCs into hepatic cells through various mechanisms [ 115 , 116 ]. Moreover, exposure of MSCs obtained from elderly donors to specific concentrations of VPA increased their immortality by specifically activating the CXCR4 signaling pathway [ 117 ].…”

Section: The Effect Of Asms On Mscsmentioning

confidence: 99%

Therapeutic Potential of Mesenchymal Stem Cells in the Treatment of Epilepsy and Their Interaction with Antiseizure Medications

Tesiye

Gol

Fadardi

et al. 2022

Cells

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Epilepsy is a life-threatening neurological disease that affects approximately 70 million people worldwide. Although the vast majority of patients may be successfully managed with currently used antiseizure medication (ASM), the search for alternative therapies is still necessary due to pharmacoresistance in about 30% of patients with epilepsy. Here, we review the effects of ASMs on stem cell treatment when they could be, as expected, co-administered. Indeed, it has been reported that ASMs produce significant effects on the differentiation and determination of stem cell fate. In addition, we discuss more recent findings on mesenchymal stem cells (MSCs) in pre-clinical and clinical investigations. In this regard, their ability to differentiate into various cell types, reach damaged tissues and produce and release biologically active molecules with immunomodulatory/anti-inflammatory and regenerative properties make them a high-potential therapeutic tool to address neuroinflammation in different neurological disorders, including epilepsy. Overall, the characteristics of MSCs to be genetically engineered, in order to replace dysfunctional elements with the aim of restoring normal tissue functioning, suggested that these cells could be good candidates for the treatment of epilepsy refractory to ASMs. Further research is required to understand the potential of stem cell treatment in epileptic patients and its interaction with ASMs.

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Augmented migration of mesenchymal stem cells correlates with the subsidiary CXCR4 variant

Cited by 10 publications

References 41 publications

Modulation of the Dental Pulp Stem Cell Secretory Profile by Hypoxia Induction Using Cobalt Chloride

Modulation of the Dental Pulp Stem Cell Secretory Profile by Hypoxia Induction Using Cobalt Chloride

Comparison the effects of hypoxia-mimicking agents on migration-related signaling pathways in mesenchymal stem cells

Therapeutic Potential of Mesenchymal Stem Cells in the Treatment of Epilepsy and Their Interaction with Antiseizure Medications

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