AuNPs-LISA, an efficient detection assay for Opisthorchis viverrini (Ov) antigen in urine

Taron, Wichit; Jamnongkan, Wassana; Techasen, Anchalee; Phetcharaburanin, Jutarop; Namwat, Nisana; Sithithaworn, Paiboon; Khuntikeo, Narong; Mukdasai, Siriboon; Sayasone, Somphou; Loilome, Watcharin; Ngeontae, Wittaya

doi:10.1016/j.talanta.2019.120592

Cited by 14 publications

(7 citation statements)

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“…Lastly, the main drawback of urine assay by ELISA is that it takes time to complete and needs experienced operators and special equipment. Adaptation of the urine assay for rapid-test platforms [ 31 , 32 ], including rapid diagnostic tests, is needed for large scale screening of opisthorchiasis.…”

Section: Discussionmentioning

confidence: 99%

Effects of day-to-day variation of Opisthorchis viverrini antigen in urine on the accuracy of diagnosing opisthorchiasis in Northeast Thailand

Worasith

Wongphutorn²,

Homwong³

et al. 2022

PLoS ONE

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Antigen detection in urine using an enzyme-linked immunosorbent assay (ELISA) is more sensitive than fecal examination for diagnosis of opisthorchiasis and for assessment of the effects of drug treatment. It is not known whether day-to-day variation of urine composition, including levels of Opisthorchis viverrini antigen, influences the urine assay. We investigated this topic with the cooperation of participants from two localities in Northeast Thailand. Project participants were screened for parasite infections for three consecutive days using the quantitative formalin-ethyl acetate concentration technique (FECT) to detect O. viverrini eggs and the urine ELISA for detection of O. viverrini antigen. A subset of participants (n = 801) with matched fecal and urine samples were analyzed for comparison of inter-day prevalence estimates and the performance of the urine assay compared against FECT for diagnosis of opisthorchiasis. The daily prevalence measured by the urine assay ranged between 29.0%-30.2% while those by FECT ranged between 11.9%-20.2%. The cumulative three-day prevalence estimate determined by the urine antigen assay was 30.3%, which was significantly higher than that by FECT (20.2%, p < 0.05). A significant positive correlation was found between the concentration of antigen in urine and fecal egg counts (p < 0.001). Overall, the urine assay had better diagnostic performance for opisthorchiasis than fecal examination by FECT. The high sensitivity plus negligible daily variation of O. viverrini antigen in urine indicates the utility of the urine assay for diagnosis, as well as population screening, of opisthorchiasis.

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Section: Discussionmentioning

confidence: 99%

Effects of day-to-day variation of Opisthorchis viverrini antigen in urine on the accuracy of diagnosing opisthorchiasis in Northeast Thailand

Worasith

Wongphutorn²,

Homwong³

et al. 2022

PLoS ONE

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“…Еще один метод, получивший распространение в диагностике паразитологических заболеваний,полимеразная цепная реакция (ПЦР), основанная на определении антигенов паразитов в различных образцах -сыворотка крови, моча, фекалии. При сравнении современных методов детекции инвазии ПЦР превосходит стандартную микроскопию образцов кала и мочи и чаще всего показывает достаточно высокую чувствительность и специфичность [41,46,[63][64][65][66]. В исследовании при сравнении со стандартными методами паразитологической диагностики ПЦР показывает значительное увеличение распространенности заболевания [67].…”

Section: молекулярно-генетические методыunclassified

Foresight in the diagnosis of trematodiasis: innovations versus routine methods

et al. 2023

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Aim. To analyze modern methods for the diagnosis of trematodiasis in experimental and epidemiological studies.Trematodiasis is a group of common parasitic diseases that are a socially sensitive health problem worldwide. According to World Health Organization, more than 250 million people are affected by trematode infections globally. The most common types of human trematode infections are diseases caused by Schistosoma, Fasciola, Clonorchis, and Opisthorchis pathogens. Diagnosis of trematodiasis is often multistage and includes identification of disease symptoms, analysis of medical history, and use of various laboratory tests. Clinical presentation of parasitic infections often varies, making a definitive diagnosis difficult. Various tools are used to diagnose trematode infections: epidemiological criteria, laboratory tests (complete blood count and blood biochemistry, serological methods), instrumental methods (abdominal X-ray and ultrasound), and parasitological techniques, which often have insufficient sensitivity and specificity. Therefore, development of modern and effective non-invasive methods for detection of trematode infections with high sensitivity and specificity, including screening in endemic regions, is relevant.The present review analyzes the results of 90 clinical trials and experimental studies on the diagnosis of trematode infections using the PubMed search engine and the eLibrary database. The review analyzes original articles published from January 1, 2015 to December 31, 2021.Most studies confirm that the absence of a standard diagnostic approach highlights obvious convenience of utilizing a combined approach to reliable diagnosis of trematodiasis. An adequate combination of different diagnostic tests makes it possible to diagnose the disease correctly, devise a correct treatment and follow-up strategy, and organize preventive measures.

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“…The catalytic activity of AuNPs can be also upgraded by the gold enhancing solution [92,94] and adenosine triphosphate. [95] Watcharin Loilome et al [96] utilized H 2 O 2 , gold enhancing solution and AuNPs to induce the catalytic activity of AuNPs. Due to the gold enhancement, the AuNPsbased assay enables detecting Opisthorchis viverrini antigen with a LOD of 23.4 ng mL -1 , substantially lower than traditional ELISA (Figure 3B).…”

Section: Noble Metal-based Nanozymesmentioning

confidence: 99%

“…Reproduced with permission. [ 96 ] Copyright 2021, Elsevier. C) Schematic illustration of a magnetic nanozyme‐linked immunosorbent assay for the detection of the influenza virus A. Reproduced with permission.…”

Section: Nanozyme‐implemented Immunoassaysmentioning

confidence: 99%

Nanobody and Nanozyme‐Enabled Immunoassays with Enhanced Specificity and Sensitivity

Wang

Xianyu

2022

Small Methods

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treatment of cancers, the second leading cause of death in the world that are hard to be treated in the terminal stages. [2] For instance, liver cancer is one of the most lethal cancers and has a poor prognosis with a 5-year survival rate of less than 20%. [3] The early detection and timely treatment of cancers are very important for the patients to save their lives. Microorganism contamination in food is the main cause of foodborne diseases and 70% of patients with foodborne illnesses get sick after taking foods or water contaminated with pathogenic microorganisms. [4] Viruses are one of the major causes of infectious diseases and various public health events like the acquired immunodeficiency syndrome (AIDS), the severe acute respiratory syndrome (SARS), and the coronavirus disease 2019 (COVID-19). [5] Therefore, the timely and rapid detection of microorganisms is of great importance to ensure human health and public safety. [6] In addition, the detections of small molecules including mycotoxins and exogenous pollutions play an important role in food safety. Mycotoxins such as aflatoxin B1, ochratoxin A, and tenuazonic acid are classified as naturally occurring carcinogens by the World Health Organization that are extremely harmful to both humans and animals. [7] They not only directly threaten human life by contaminating food, but also indirectly affect human health through the food chain by contaminating feeds of animals. [8,9] Commonly used immunoassays include enzyme-linked immunosorbent assay (ELISA), quantum dot fluorescence immunoassay, fluorescence polarization immunoassay, colloidal gold immunoassay, chemiluminescent immunoassay (CLIA), electrochemical immunoassay, and microarray-based immunoassay. In spite of the different formats of immunoassays, they share the same principle that relies on the specific binding between antigenic determinants and antibody recognition sites. Therefore, it is of great significance to prepare specific antibodies with high affinity towards the target molecules to improve the specificity of immunoassays. Conventional immunoassays generally use polyclonal and monoclonal antibodies, but both types of antibodies have limitations such as poor specificity, low stability, and complicated preparation. To meet these challenges, a new type of antibody called "nanobody" has emerged. Nanobody is the heavy-chain-only antibody with the advantages of small size, high specificity, mass expression and high stability, which can act as a substitute for conventional antibodies in immunoassays and a promising low-cost immuno-affinity detection reagent.Immunoassay as a rapid and convenient method for detecting a variety of targets has attracted tremendous interest with its high specificity and sensitivity. Among the commonly used immunoassays, enzyme-linked immunosorbent assay has been widely used as a gold standard method in various fields that consists of two main components including a recognition element and an enzyme label. With the rapid advances in nanotechnology, nanobodies and nanozym...

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AuNPs-LISA, an efficient detection assay for Opisthorchis viverrini (Ov) antigen in urine

Cited by 14 publications

References 34 publications

Effects of day-to-day variation of Opisthorchis viverrini antigen in urine on the accuracy of diagnosing opisthorchiasis in Northeast Thailand

Effects of day-to-day variation of Opisthorchis viverrini antigen in urine on the accuracy of diagnosing opisthorchiasis in Northeast Thailand

Foresight in the diagnosis of trematodiasis: innovations versus routine methods

Nanobody and Nanozyme‐Enabled Immunoassays with Enhanced Specificity and Sensitivity

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