Aurora B and Cyclin B Have Opposite Effects on the Timing of Cytokinesis Abscission in Drosophila Germ Cells and in Vertebrate Somatic Cells

Mathieu, Juliette; Cauvin, Clothilde; Moch, Clara; Radford, Sarah J.; Sampaio, Paula; Perdigoto, Carolina N.; Schweisguth, François; Bardin, Allison J.; Sunkel, Cláudio E.; McKim, Kim S.; Échard, Arnaud; Huynh, Jean‐René

doi:10.1016/j.devcel.2013.07.005

Cited by 93 publications

(146 citation statements)

References 61 publications

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“…In fruit flies, for example, the number of germ cells in each Drosophila egg chamber is regulated by abscission inhibition during differentiation. 16 Finally, G1-coupled abscission essentially postulates that the last step of the cell cycle, as we traditionally see it, is in fact regulated by the following cycle (Fig. 4B).…”

Section: Resultsmentioning

confidence: 78%

Cytokinetic abscission is an acute G1 event

et al. 2014

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Animal cell division ends with the cutting of the microtubule and membrane intercellular bridge connecting the 2 daughter cells. This process, known as cytokinetic abscission (abscission), is widely regarded as the last step of cytokinesis, i.e., the last step of the cell cycle. Major breakthroughs have been recently achieved, illuminating mechanistic aspects of abscission; however, the timing of abscission with respect to the mammalian cell cycle remains unclear. In this study, we carefully measured the onset and progression of abscission in dividing cells expressing a G1 reporter. We conclude that abscission commences long after cells enter the G1 phase. Affiliating abscission with G1 is beyond semantics since it essentially postulates that the last step of the cell cycle is regulated in, and probably by, the following cycle.

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Section: Resultsmentioning

confidence: 78%

Cytokinetic abscission is an acute G1 event

et al. 2014

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“…Loss of CycB leads to GSC self-renewal and maintenance defects (Wang and Lin, 2005). CycB is also required for the abscission of the CB from the GSCs, and for CB proliferation (Chau et al, 2012; Mathieu et al, 2013). Thus, CycB plays a pivotal role in the early germ line to ensure GSC self-renewal, maintenance, and proliferation of the GSC daughter.…”

Section: Introductionmentioning

confidence: 99%

Transient transcriptional silencing alters the cell cycle to promote germline stem cell differentiation in Drosophila

Flora

Schowalter

Wong-Deyrup

et al. 2018

Developmental Biology

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Transcriptional silencing is a conserved process used by embryonic germ cells to repress somatic fate and maintain totipotency and immortality. In Drosophila, this transcriptional silencing is mediated by polar granule component (pgc). Here, we show that in the adult ovary, pgc is required for timely germline stem cell (GSC) differentiation. Pgc is expressed transiently in the immediate GSC daughter (pre-cystoblast), where it mediates a pulse of transcriptional silencing. This transcriptional silencing mediated by pgc indirectly promotes the accumulation of Cyclin B (CycB) and cell cycle progression into late-G2 phase, when the differentiation factor bag of marbles (bam) is expressed. Pgc mediated accumulation of CycB is also required for heterochromatin deposition, which protects the germ line genome against selfish DNA elements. Our results suggest that transient transcriptional silencing in the pre-cystoblast “re-programs” it away from self-renewal and toward the gamete differentiation program.

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“…RCs are required for fertility in both insects and mammals, but current knowledge of the regulation of these structures is limited (Robinson et al, 1994;Haglund et al, 2011). The scaffolding molecules Anillin and Cindr, the central spindle component Pavarotti, as well as actin and myosin have been shown to localize to RCs (Field and Alberts, 1995;Hime et al, 1996;Adams et al, 1998;Haglund et al, 2010;Eikenes et al, 2013), but little is known about the molecular mechanisms regulating incomplete cytokinesis in vivo Greenbaum et al, 2011;Haglund et al, 2011;Mathieu et al, 2013;Yamamoto et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Src64 controls a novel actin network required for proper ring canal formation in the Drosophila male germline

et al. 2015

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In many organisms, germ cells develop as cysts in which cells are interconnected via ring canals (RCs) as a result of incomplete cytokinesis. However, the molecular mechanisms of incomplete cytokinesis remain poorly understood. Here, we address the role of tyrosine phosphorylation of RCs in the Drosophila male germline. We uncover a hierarchy of tyrosine phosphorylation within germline cysts that positively correlates with RC age. The kinase Src64 is responsible for mediating RC tyrosine phosphorylation, and loss of Src64 causes a reduction in RC diameter within germline cysts. Mechanistically, we show that Src64 controls an actin network around the RCs that depends on Abl and the Rac/SCAR/Arp2/3 pathway. The actin network around RCs is required for correct RC diameter in cysts of developing germ cells. We also identify that Src64 is required for proper germ cell differentiation in the Drosophila male germline independent of its role in RC regulation. In summary, we report that Src64 controls actin dynamics to mediate proper RC formation during incomplete cytokinesis during germline cyst development in vivo.

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Aurora B and Cyclin B Have Opposite Effects on the Timing of Cytokinesis Abscission in Drosophila Germ Cells and in Vertebrate Somatic Cells

Cited by 93 publications

References 61 publications

Cytokinetic abscission is an acute G1 event

Cytokinetic abscission is an acute G1 event

Transient transcriptional silencing alters the cell cycle to promote germline stem cell differentiation in Drosophila

Src64 controls a novel actin network required for proper ring canal formation in the Drosophila male germline

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