Auslegung, Simulation und Validierung einer Apparatur zur Aufbringung hoher Scherbelastungen auf Zellen in Suspension

Volumetric mass density measurements of mesenchymal stem cells in suspension using a density meter

“…For a cell suspension, it is a mixture of a continuous fluid (the cell suspension medium) and a dispersed phase (the cells) that displaces the medium. 5

Figure 4 Volumetric mass density, dynamic viscosity, osmolality, and pH of different DPBS-LSM mixtures The exact fluid properties are listed in Table S2 .

Figure 5 Concept of density centrifugation With the volumetric mass density of the cells being higher than the volumetric mass density of the DPBS (1.0052 g/cm 3 ) that carries the cells, they always sink to the interface of DPBS and DPBS-LSM during centrifugation.

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“…For a cell suspension, it is a mixture of a continuous fluid (the cell suspension medium) and a dispersed phase (the cells) that displaces the medium. 5 …”

“…The cell media used to investigate the resistance of cells to mechanical shear and strain in an experimental setting need to be adjusted for the volumetric mass density of the cells. 5 Furthermore, the technical procedures mentioned above can be investigated using computational fluid dynamics (CFD) simulations to gain additional insights and reduce development time and costs compared to trial-and-error experiments. They allow the investigation of mechanical strain and mechanical forces and can predict the trajectory of individual cells in cell suspensions using particle models based on the Euler-Lagrange approach.…”