Australian propolis ethanol extract exerts antibacterial activity against methicillin-resistant Staphylococcus aureus by mechanisms of disrupting cell structure, reversing resistance, and resisting biofilm

Wang, Fei; Liu, Hui; Li, Junya; Zhang, Wenwen; Jiang, Bin; Han, Xuan

doi:10.1007/s42770-021-00547-7

Cited by 25 publications

(15 citation statements)

References 49 publications

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“…However, following RPE treatment, the normal shape of the cells is disrupted and the contents are released ( Figure 4 C), suggesting that the action of RPE against S. aureus and MRSA is via damage to the cell wall—a theory further supported by the leakage of DNA and protein studies that we conducted. These results were in agreement with the conclusions of previous studies [ 47 , 48 ].…”

Section: Discussionsupporting

confidence: 94%

Antibacterial Activity of Chinese Red Propolis against Staphylococcus aureus and MRSA

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Escalada

et al. 2022

Molecules

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The antibacterial activity of propolis has long been of great interest, and the chemical composition of propolis is directly dependent on its source. We recently obtained a type of propolis from China with a red color. Firstly, the antibacterial properties of this unusual propolis were determined against Staphylococcus aureus and methicillin-resistant Staphylococcus aureus (MRSA). Studies on its composition identified and quantified 14 main polyphenols of Chinese red propolis extracts (RPE); quantification was carried out using liquid chromatography triple quadrupole tandem mass spectrometry (LC-QQQ-MS/MS) and RPE was found to be rich in pinobanksin, pinobanksin-3-acetate, and chrysin. In vitro investigations of its antibacterial activity revealed that its activity against S. aureus and MRSA is due to disruption of the cell wall and cell membrane, which then inhibits bacterial growth. Despite its similar antibacterial activities against S. aureus and MRSA, metabolomic analysis further revealed the effects of RPE on bacteria metabolism were different. The untargeted metabolomic results showed that a total of 7 metabolites in 12 metabolic pathways had significant changes (Fold change > 2, p < 0.05 *) after RPE treatment in S. aureus, while 11 metabolites in 9 metabolic pathways had significant changes (Fold change > 2, p < 0.05 *) after RPE treated on MRSA. Furthermore, RPE downregulated several specific genes related to bacterial biofilm formation, autolysis, cell wall synthesis, and bacterial virulence in MRSA. In conclusion, the data obtained indicate that RPE may be a promising therapeutic agent against S. aureus and MRSA.

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Section: Discussionsupporting

confidence: 94%

Antibacterial Activity of Chinese Red Propolis against Staphylococcus aureus and MRSA

Sun

Escalada

et al. 2022

Molecules

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“…The experimental method was slightly modified according to the previously described method (Wang et al, 2021). Briefly speaking, after MRSA strains were cultured overnight in TSB containing 2% glucose, 100 µl of the overnight culture was added to 96-well plates.…”

Section: Cell Adhesion Assaymentioning

confidence: 99%

Small-Molecule Compound SYG-180-2-2 to Effectively Prevent the Biofilm Formation of Methicillin-Resistant Staphylococcus aureus

et al. 2022

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The resistance of methicillin-resistant Staphylococcus aureus (MRSA) has augmented due to the abuse of antibiotics, bringing about difficulties in the treatment of infection especially with the formation of biofilm. Thus, it is essential to develop antimicrobials. Here we synthesized a novel small-molecule compound, which we termed SYG-180-2-2 (C21H16N2OSe), that had antibiofilm activity. The aim of this study was to demonstrate the antibiofilm effect of SYG-180-2-2 against clinical MRSA isolates at a subinhibitory concentration (4 μg/ml). In this study, it was showed that significant suppression in biofilm formation occurred with SYG-180-2-2 treatment, the inhibition ranged between 65.0 and 85.2%. Subsequently, confocal laser scanning microscopy and a bacterial biofilm metabolism activity assay further demonstrated that SYG-180-2-2 could suppress biofilm. Additionally, SYG-180-2-2 reduced bacterial adhesion and polysaccharide intercellular adhesin (PIA) production. It was found that the expression of icaA and other biofilm-related genes were downregulated as evaluated by RT-qPCR. At the same time, icaR and codY were upregulated when biofilms were treated with SYG-180-2-2. Based on the above results, we speculate that SYG-180-2-2 inhibits the formation of biofilm by affecting cell adhesion and the expression of genes related to PIA production. Above all, SYG-180-2-2 had no toxic effects on human normal alveolar epithelial cells BEAS-2B. Collectively, the small-molecule compound SYG-180-2-2 is a safe and effective antibacterial agent for inhibiting MRSA biofilm.

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“…Thus causing damage of the cell walls and bacteria form [107], alteration of the physico-chemical properties of surface layer cells [108], disruption of membrane potential and bioenergetic status ( inhibition of adenosine triphosphate (ATP) production). In addition, propolis extract inhibit biofilm formation [109]), and posses anti-quorum sensing activity [110], Recently, Wang and co workers [111] demonstrated that Australian propolis ethanol extract (APEE) possesse anti-planktonic, anti-biofilm and anti-adhesive activities against MRSA strains. Otherwise, Vadillo-Rodríguez and collaborators 2021 suggested that initial mechanism of action of propolis resulting from interaction between different propolis components and bacterial cell wall structures.…”

Section: Antibacterial Activitymentioning

confidence: 99%

“…Disrupting cell structure; Inhibitory activity against planktonic bacterial cells and biofilm. [125].…”

Section: Australian Propolis Ethanol Extract (Apee)mentioning

confidence: 99%

“…Wang and his colleagues [111], demonstrated that Australian propolis ethanol extract (APEE) decrease β-lactamase activity and inhibit expression of specific drug-resistant protein; PBP2a (penicillin binding protein 2a) in MRSA strains. Same authors suggested that combination of APEE with β-lactam antibiotics (ampicillin, methicillin…..),consequently, increasing therapeutic effect of antibiotic.…”

Section: Combination Effect Of Propolis and Antibioticmentioning

confidence: 99%

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Biological Activity of Propolis: An Update

Benhanifia¹,

SOLTANI²

2022

Preprint

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Antibiotic resistance was described soon discovery of penicillin by Fleming. In recent years, there has been increasing research interest in the development of alternatives to address this problem. Natural products, mainly, have long been considered as sources of drugs, and a great part of pharmaceuticals available in modern medicine are directly or indirectly derived from natural sources. propolis been used to treat human diseases since ancient times, propolis, is a natural resinous mixture produced by honeybees (Apis mellifera) from collected parts of plants, he is increasingly recognized by their wide range of biological and pharmacological properties, As anti- infective agent, combination synergy with standard antibiotics could be very promising alternative strategy

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Australian propolis ethanol extract exerts antibacterial activity against methicillin-resistant Staphylococcus aureus by mechanisms of disrupting cell structure, reversing resistance, and resisting biofilm

Cited by 25 publications

References 49 publications

Antibacterial Activity of Chinese Red Propolis against Staphylococcus aureus and MRSA

Antibacterial Activity of Chinese Red Propolis against Staphylococcus aureus and MRSA

Small-Molecule Compound SYG-180-2-2 to Effectively Prevent the Biofilm Formation of Methicillin-Resistant Staphylococcus aureus

Biological Activity of Propolis: An Update

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