Author Correction: Lipoarabinomannan antigenic epitope differences in tuberculosis disease subtypes

Magni, R.; Rruga, Fatlum; Alsaab, Fahad M.; Sharif, Sara; Howard, M. Keith; Espina, Virginia; Kim, Brianna; Lepene, Benjamin; Lee, Gwenyth; Alayouni, Mohamad A.; Steinberg, Hannah; Araujo, Robyn P.; Kashanchi, Fatah; Riccardi, F; Morreira, Sargento; Araujo, Antonia; Poli, Fernando; Jaganath, Devan; Semitala, Fred C.; Worodria, William; Andama, Alfred; Choudhary, Alok; Honnen, William J.; Petricoin, Emanuel F.; Cattamanchi, Adithya; Colombatti, Raffaella; Waard, Jacobus H. de; Oberhelman, Richard A.; Pinter, Abraham; Gilman, Robert H.; Liotta, Lance A.; Luchini, Alessandra

doi:10.1038/s41598-021-98304-1

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“…The major question regarding the LAM assay is whether it can be used as a reliable TB biomarker. Specifically, it is unclear whether the LAM levels in the urine of HIV negative patients- are simply too low to be measured by the current test [ 8 , 25 , 26 ].. Our findings revealed that urine LAM concentrations in HIV-negative TB patients were generally low. Specifically, in 39 out of 47 positive cases, LAM concentrations ranged from 1 to 20 pg/mL, which could only be detected by our highly sensitive CLIA-LAM assay (Data from supplement Table 1 ).…”

Section: Discussionmentioning

confidence: 68%

Improving the diagnosis of active tuberculosis: a novel approach using magnetic particle-based chemiluminescence LAM assay

Li,

Ru,

Wei

et al. 2024

BMC Pulm Med

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Objectives Tuberculosis (TB) is a significant global health concern, given its high rates of morbidity and mortality. The diagnosis using urine lipoarabinomannan (LAM) primarily benefits HIV co-infected TB patients with low CD4 counts. The focus of this study was to develop an ultra-sensitive LAM assay intended for diagnosing tuberculosis across a wider spectrum of TB patients. Design & Methods To heighten the sensitivity of the LAM assay, we employed high-affinity rabbit monoclonal antibodies and selected a highly sensitive chemiluminescence LAM assay (CLIA-LAM) for development. The clinical diagnostic criteria for active TB (ATB) were used as a control. A two-step sample collection process was implemented, with the cutoff determined initially through a ROC curve. Subsequently, additional clinical samples were utilized for the validation of the assay. Results In the assay validation phase, a total of 87 confirmed active TB patients, 19 latent TB infection (LTBI) patients, and 104 healthy control samples were included. Applying a cutoff of 1.043 (pg/mL), the CLIA-LAM assay demonstrated a sensitivity of 55.2% [95%CI (44.13%~65.85%)], and a specificity of 100% [95%CI (96.52%~100.00%)], validated against clinical diagnostic results using the Mann-Whitney U test. Among 11 hematogenous disseminated TB patients, the positive rate was 81.8%. Importantly, the CLIA-LAM assay consistently yielded negative results in the 19 LTBI patients. Conclusion Overall, the combination of high-affinity antibodies and the CLIA method significantly improved the sensitivity and specificity of the LAM assay. It can be used for the diagnosis of active TB, particularly hematogenous disseminated TB.

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Section: Discussionmentioning

confidence: 68%

Improving the diagnosis of active tuberculosis: a novel approach using magnetic particle-based chemiluminescence LAM assay

Li,

Ru,

Wei

et al. 2024

BMC Pulm Med

View full text Add to dashboard Cite

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Author Correction: Lipoarabinomannan antigenic epitope differences in tuberculosis disease subtypes

Abstract: As a result of the changes, the Affiliations have been renumbered.The original Article and accompanying Supplementary Information file have been corrected.

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Improving the diagnosis of active tuberculosis: a novel approach using magnetic particle-based chemiluminescence LAM assay

Improving the diagnosis of active tuberculosis: a novel approach using magnetic particle-based chemiluminescence LAM assay

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