Author response: Probing the catalytic functions of Bub1 kinase using the small molecule inhibitors BAY-320 and BAY-524

Baron, Anna P.; Schubert, Conrad von; Cubizolles, Fabien; Siemeister, Gerhard; Hitchcock, Marion; Mengel, Anne; Schröder, Jörg; Fernández-Montalván, Amaury E.; Nussbaum, Franz von; Mumberg, Dominik; Nigg, Erich A.

doi:10.7554/elife.12187.022

Cited by 2 publications

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“…The apparent absence of a congression defect in Bub1 -/cells is consistent with previous work in RPE1 cells treated with Bub1 kinase inhibitors, but is perhaps surprising given the known role of Bub1 in error correction through the Histone2A-Sgo1/PP2A-Aurora B pathway [7]. We therefore generated multiple malorientated attachments by releasing cells from a nocodazole arrest and measured the time to completion of congression and anaphase onset.…”

Section: Correspondencesupporting

confidence: 84%

“…One possibility is that the KBB and RZZ pathways monitor different attachment states [2]. Furthermore, the contribution of the KBB pathway to SAC signalling and error correction may vary between cell types or state of cellular transformation [2,7]. Future work will be needed to understand the relative roles of KBB and RZZ in these different contexts.…”

Section: Correspondencementioning

confidence: 99%

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Bub1 is not essential for the checkpoint response to unattached kinetochores in diploid human cells

et al. 2018

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Error-free chromosome segregation during mitosis depends on a functional spindle assembly checkpoint (SAC). The SAC is a multi-component signalling system that is recruited to unattached or incorrectly attached kinetochores to catalyse the formation of a soluble inhibitor, known as the Mitotic Checkpoint Complex (MCC), which binds and inhibits the anaphase promoting complex (APC/C) [1]. We have previously proposed that two separable pathways, composed of KNL1-Bub3-Bub1 (KBB) and Rod-Zwilch-Zw10 (RZZ), recruit Mad1-Mad2 complexes to human kinetochores to activate the SAC [2]. Although Bub1 is absolutely required for checkpoint signalling in yeast (which lack RZZ), there is conflicting evidence as to whether this is the case in human cells based on siRNA studies [2-5]. Here we show that, while Bub1 is required for recruitment of BubR1, it is not strictly required for the checkpoint response to unattached kinetochores in diploid human cells.

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Section: Correspondencesupporting

confidence: 84%

Section: Correspondencementioning

confidence: 99%

Bub1 is not essential for the checkpoint response to unattached kinetochores in diploid human cells

et al. 2018

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The Anaphase Promoting Complex/Cyclosome (APC/C): A Versatile E3 Ubiquitin Ligase

Curtis

Bolaños-García

2019

Subcellular Biochemistry

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Amyloid beta (Aβ), Adenovirus (Ad), Alzheimer's Disease (AD), Adriamycin (ADM), Acute Myeloid Leukemia (AML), Anaphase Promoting Complex/Cyclosome (APC/C), Adult T-cell leukaemia/lymphoma (ATL), Adenosine Triphosphate (ATP), Ataxia telangiectasia and Rad3-related protein (ATR), BRCA1-associated RING domain protein 1 (Bard1), Brain-Specific Kinase 2 (BRSK2), Budding uninhibited by benzimidazoles 1 (Bub1), Budding uninhibited by benzimidazoles 3 (Bub3), Budding uninhibited by benzimidazoles related 1 (BubR1), Chicken Anemia virus (CAV), Chicken Anemia Virus-Apoptin (CAV-Apoptin), Cyclic AMP response element binding protein (CBP), Cell division cycle 5 (Cdc5), Cell division cycle 6 protein (Cdc6), Cell Division Cycle 7 (Cdc7), Cell division cycle protein 20 (Cdc20), Cell division cycle protein 23 (Cdc23), Cell division cycle protein 26 (Cdc26), Cell division cycle protein 27 (Cdc27), Cell division control protein 42 (Cdc42), Cell Division Cycle 55 (Cdc55), Cadherin 1 (Cdh1), Cyclin-dependent kinase (Cdk), Cyclin-dependent kinase 1 (Cdk1), Cyclin-dependent kinase 4 (Cdk4), Cyclin-dependent kinase 5 (Cdk5), Chromatin licensing and DNA replication factor 1 (Cdt1), Centromere-associated protein E (CENP-E), Centromere-associated protein F (CENP-F), Checkpoint kinase 1 (Chk1), Chromosome instability and karyogamy protein 1 (Cik1), Casein kinase 1 (CK1), Casein kinase 1 delta (CK1δ), Cytoskeleton-associated protein 2 (CKAP2), Conditional Knockout (cKO), Cyclindependent kinases regulatory subunit 1 (Cks1), Central nervous system (CNS), Coimmunoprecipitation (co-IP), Chromosomal passenger complex (CPC), Cryogenic electron microscopy (cryo-EM), Destruction box (D-box), Damaged DNA binding protein 1 (DDB1), Cisplatin (DDP), Deoxyribonucleic acid (DNA), Deoxyribonucleotide triphosphate (dNTPS), Deoxythymidine triphosphate (dTTP) early (E), Adenovirus early region 1A (E1A), E2F Transcription Factor 1 (E2F1), E2F Transcription Factor 3 (E2F3), Early Region 4 Open Reading Frame 4 (E4orf4), Excitatory amino acid (EAA), Epithelial Cell Transforming 2 (Ect2), Euchromatic Histone Lysine Methyltransferase 2 (EHMT2), Electron microscopy (EM)Early mitotic inhibitor (Emi1), Endoplasmic Reticulum (ER), Eyes Absent 1 (EYA1), FANCD2-associated nuclease 1 (FAN1), Filaments in between nuclei protein 1 (Fin1), Forkhead box M1 (FoxM1), Fizzy-related protein homolog (Fzr), Gap 1 (G1), Gap 2 (G2), Glucose-6-phosphate dehydrogenase (G6PD), Granule cell progenitors (GCPs), Glucagonlike peptide (GLP), Glucagon-like peptide-1 (GLP1), Glutaminase 1 (GLS1), Glutamate receptor 1 (GluR1), glioblastoma stem-like cells (GSCs), Glutathione (GSH), Glutathione disulfide (GSSG), Human Adenovirus (HAd), Hepatitis B Virus (HBV), Hepatocellular carcinoma (HCC), Human cytomegalovirus (HCMV), Homologous to the E6-AP Carboxyl Terminus (HECT), Human Gyrovirus-Apoptin (HGyv-Apoptin), Hyaluronan-mediated motility

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Author response: Probing the catalytic functions of Bub1 kinase using the small molecule inhibitors BAY-320 and BAY-524

Cited by 2 publications

References 0 publications

Bub1 is not essential for the checkpoint response to unattached kinetochores in diploid human cells

Bub1 is not essential for the checkpoint response to unattached kinetochores in diploid human cells

The Anaphase Promoting Complex/Cyclosome (APC/C): A Versatile E3 Ubiquitin Ligase

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