Autologous stem cell therapy to treat chronic ulcer in heifer- A case study

Abstract

Aim : The study was conducted to reveal the efficacy of Bone marrow derived mesenchymal stem cells (BM-MSCs) based therapy in healing of chronic non-healing and ulcerative wound in bovine species. Materials and Methods: One 2 years old Jersey heifer affected with chronic ulcerative wound involving full thickness skin and under lying muscle at dorsal side of lumbar region since four months at the time of presentation. Bone marrow was collected from tibia, cultured and grown and after achievement of optimum confluence it was applied at the site. Different parameters of clinical, physiological, haematological, biochemical, histochemical, histological, tensile strength and photographic evaluations were done during the study period. Results:The estimated values of above mentioned parameters on zero day and after healing (18 days) showed significant difference (P<0.05) in relation to collagen content, tensile strength and physical characteristics of wound like extent of wound, size of wound, type of exudates and photography. But clinical, haematological and biochemical data showed no significant difference. Conclusion: The BM-MSCs were the main pioneers to bring the chronic ulcerative wound towards healing. The procedure is simple, safe and effective in bringing out healing without showing any adverse effect on host. Key words: BM-MSCs, bovine, chronic ulcerative wound healing. Hence one clinical trial was made with Introduction autologous bone marrow derived stem cell (BMChronic non-healing or slow healing ulcers MSCs) therapy and to know the progress and duration represent a major health burden and continue to pose a of healing with this application. challenge not only to the patient owners but to the Materials and Methodstreating physician and medical procedures as a whole. The healing of wound occurs by primary intention andThe owner was fully informed and written secondary intention [1,2,3]. The therapeutic application consent was obtained about the clinical trial, risk and of autologous BM derived MSCs (Bone marrow benefits of the proposed cell based therapy. It was also derived mesenchymal stem cells) have revolutionized approved by the Institutional Animal Ethics the field of regenerative medicine. The BM-MSCs Committee of the institution since it was a clinical case were been used for the treatment of chronic wound in and related to Agricultural Production Research. diabetic patients [4], for non-healing ulcers of lower Measurement of the wound dimension was taken extremities in human being [5], cutaneous radiation along with swab from wound bed for bacteriological syndrome in minipig model [6] and in dogs [7] proved culture and sensitive test (Fig.1). Biopsy was done successful in their findings. A clinical case of 2 years from wound bed for histopathological and old Jersey heifer was presented with one large size histochemical study. Wound was washed, cleaned and chronic non-healing wound at lumbar region since 4 dressed with fly repellent spray. Under peroneal nerve months. It was treated with dif...

An Update on Applications of Cattle Mesenchymal Stromal Cells

“…The first report of a case study in which autologous BM-derived MSCs were used to treat a chronic ulcer in a heifer dates back to 2012 [ 10 ]. A 2-year-old Jersey heifer had been suffering from a chronic nonhealing ulcerative wound involving full-thickness skin and underlying muscle in the lumbar region for 4 months.…”

“…The content of collagen was increased after stem cell therapy, and the healed tissue was progressing towards physiological stretchability and tensile strength. The 4-month-old chronic wound healed within 18 days, indicating that MSCs application could be an effective therapeutic approach for nonhealing chronic wounds [ 10 ].…”

“…Bovine MCSs have been isolated and characterised ( Figure 1 ) from different adult and foetal tissues, including bone marrow (BM) [ 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 ], endometrium (EN) [ 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 ], adipose tissue (AT) [ 29 , 30 , 31 , 32 , 34 , 37 , 49 , 50 , 51 ,…”