Automated annotation removal in agar plates

Abstract

Agar plates are widely used in the biomedical field as a medium in which to artificially grow bacteria, algae or fungi. Agar plates (Petri dishes) are used routinely in microbiology laboratories in order to identify the type of micro-organism responsible for infections. Such diagnoses are based on counting the number and type of bacterial colonies growing in the Petri dish. The count of bacterial colonies is a time consuming task prone to human error, so interest in automated counting systems has increased in the recent years. One of the difficulties of automatizing the counting process is the presence of markers and annotations made in the lower part of the agar plate. Efficient removal of such markers can increase the accuracy of the bacterial counting system. This article introduces a fast method for detection, segmentation and removal of annotations in agar plates that improves the results of existing bacterial colony counting algorithms.