Automated depth-scanning confocal Raman microspectrometer for rapidin vivo determination of water concentration profiles in human skin

Caspers, Peter; Lucassen, Gerald W.; Bruining, H. A.; Puppels, Gerwin J.

doi:10.1002/1097-4555(200008/09)31:8/9<813::aid-jrs573>3.0.co;2-7

Cited by 250 publications

(233 citation statements)

References 28 publications

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“…agnostic applications has been well investigated and demonstrated, notably in dermal applications [3][4][5][6] but also for many other anatomical sites, including cervix [7,8], skin [9][10][11], lung [12,13], brain [14], oesophagus [15,16], colon [17], prostate [18], nasopharynx [19], larynx [20], oral [21], breast [22,23] and liver [24]. The detailed information of the molecular structure and composition of the tissue provided by Raman and Fourier Transform Infrared absorption (FTIR) spectroscopy ultimately promises an analysis of disease origin and progression.…”

Section: Biophotonicsmentioning

confidence: 99%

Improved protocols for vibrational spectroscopic analysis of body fluids

Bonnier¹,

Petitjean

Baker

et al. 2013

Journal of Biophotonics

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The applications of vibrational spectroscopy to the examination of human blood serum are explored. Although FTIR spectra can be recorded in aqueous solutions at (gelatin) concentrations as low as 100 mg/L, the high-wavenumber region remains obscured by water absorption. Using Raman spectroscopy, high quality spectra of gelatine solutions as low as 10 mg/L can be achieved, also covering the high-wavenumber regions. In human serum, spectral profiles are weak and partially obscured by water features. Dried deposits are shown to be physically and chemically inhomogeneous resulting in reduced measurement reproducibility. Concentration of the serum using commercially available centrifugal filter devices results in an improvement in the spectral intensity and quality. Additionally, in Raman spectroscopy, reduced background and significantly enhanced signal collection is achievable by measurement in an inverted geometry. The improved protocols for spectroscopic measurement of human serum are applicable to a range of bodily fluids and should accelerate potential clinical applications

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Section: Biophotonicsmentioning

confidence: 99%

Improved protocols for vibrational spectroscopic analysis of body fluids

Bonnier¹,

Petitjean

Baker

et al. 2013

Journal of Biophotonics

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show abstract

“…It is a routine technique for fingerprinting and identifying chemicals and acts as a standard method of analytical pharmacy and chemistry 8 . The potential of vibrational spectroscopy for diagnostic applications has been demonstrated, notably in dermal applications [9][10][11] , as well as for in vitro screening of toxicological effects of ionising radiation 12 , nanoparticles 13,14 and the action of chemotherapeutic agents 15 . Raman and infrared (IR) spectroscopy additionally provide detailed information of the molecular structure and composition of the tissue, ultimately promising an analysis of disease origin.…”

Section: Introductionmentioning

confidence: 99%

Raman spectroscopic mapping for the analysis of solar radiation induced skin damage

Ali¹,

Bonnier²,

Ptasiński³

et al. 2013

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“…9 -12 The accurate detection of BCC has attracted much attention from clinical dermatologists since it is difficult to distinguish BCC tissue from surrounding noncancerous tissue. [13][14][15][16] The routine diagnostic technique used for the detection of BCC is pathological examination of biopsy samples. This involves removal of tissue from suspected areas, which is then sliced and stained to enable the pathologist to identify morphological abnormalities.…”

Section: Introductionmentioning

confidence: 99%