Automated High-Throughput Matrix Assisted Laser Desorption Ionization Mass Spectrometry Methodology for Formulation Assessment of Polyethylene-Glycol-Conjugated Cytokine Proteins

Real-Time Determination of Molecular Weight: Use of MaDDOSY (Mass Determination Diffusion Ordered Spectroscopy) to Monitor the Progress of Polymerization Reactions

“…7 Its use has become more widespread, evidenced by Pirrone et al, who used it for the rapid screening of poly(ethylene glycol) conjugated cytokines. 8 Indeed, there are now commercially available MALDI spotters capable of integration with existing systems 9 to reduce preparation and analysis time.…”

“…Automated MALDI analysis has shown some promise since its inception by Meier et al, where a home-built MALDI spotter was integrated with a high-performance liquid chromatography (HPLC) system and applied to study polymer end groups . Its use has become more widespread, evidenced by Pirrone et al, who used it for the rapid screening of poly(ethylene glycol) conjugated cytokines . Indeed, there are now commercially available MALDI spotters capable of integration with existing systems to reduce preparation and analysis time.…”