Automated lab-on-valve sequential injection ELISA for determination of carbamazepine

Ramos, Inês I.; Carl, Peter; Schneider, R.; Segundo, Marcela A.

doi:10.1016/j.aca.2019.05.017

Cited by 13 publications

(6 citation statements)

References 40 publications

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“…The configuration of the SI-LOV (MicroSIA, FIAlab instruments, Inc., Bellevue, WA, USA) system used for nanoparticles quantification (Figure S1) is described in Supporting Information. Optical detection and quantification of nanoparticles was performed in the LOV integrated detection unit (further details on Supporting Information) ,, by measuring the light attenuation (i.e., light lost due to scattering and/or absorbance events) when nanoparticles were present in the optical path.…”

Section: Experimental Sectionmentioning

confidence: 99%

“…LOV accommodates sampling, online dilution and mixing by flow reversal, and online detection as it integrates an optical detection unit, enabling several operations in a single setup. This system has successfully been employed to set simpler, faster and less-laborious quantification of small molecules, sorbent-based sample pretreatment, , and molecular recognition procedures. , Furthermore, considering the need of ensuring minimal stress and dilution during the analysis of organic NPs, the LOV platform emerges as a valuable alternative for NPs characterization as it offers (i) inert bore conduits ( ca . 1.0 mm), larger than conventional size-exclusion columns and ultrafiltration/dialysis membranes, (ii) low operation flow rates ( e.g ., 1 μL s –1 ), and (iii) short distance (10 mm) between the sampling port and the detection unit.…”

mentioning

confidence: 99%

“…Considering the above-mentioned challenges, improved, alternative, and orthogonal procedures to the currently available strategies for NPs concentration measurements have been pursued. , Nevertheless, label free, simpler, and fit for purpose methods suitable to characterize the number of organic NPs in formulation batches for routine quality control operations are yet required. In this context, an automated, miniaturized and simple method to quantify NPs resorting to the sequential injection lab-on-valve (SI-LOV) system , is proposed. LOV is a mesofluidic platform that provides precise handling of small volumes (μL) of samples/reagents in rigid miniaturized channels resorting to flow programming and to a multiposition selection valve, , thus affording robust and reproducible operations with downscaled consumption of samples/reagents .…”

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confidence: 99%

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Lab-on-Valve Automated and Miniaturized Assessment of Nanoparticle Concentration Based on Light-Scattering

et al. 2023

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Nanoparticles (NPs) concentration directly impacts the dose delivered to target tissues by nanocarriers. The evaluation of this parameter is required during NPs developmental and quality control stages, for setting dose–response correlations and for evaluating the reproducibility of the manufacturing process. Still, faster and simpler procedures, dismissing skilled operators and post-analysis conversions are needed to quantify NPs for research and quality control operations, and to support result validation. Herein, a miniaturized automated ensemble method to measure NPs concentration was established under the lab-on-valve (LOV) mesofluidic platform. Automatic NPs sampling and delivery to the LOV detection unit were set by flow programming. NPs concentration measurements were based on the decrease in the light transmitted to the detector due to the light scattered by NPs when passing through the optical path. Each analysis was accomplished in 2 min, rendering a determination throughput of 30 h–1 (6 samples h–1 for n = 5) and only requiring 30 μL (≈0.03 g) of NPs suspension. Measurements were performed on polymeric NPs, as these represent one of the major classes of NPs under development for drug-delivery aims. Determinations for polystyrene NPs (of 100, 200, and 500 nm) and for NPs made of PEGylated poly-d,l-lactide-co-glycolide (PEG–PLGA, a biocompatible FDA-approved polymer) were accomplished within 108–1012 particles mL–1 range, depending on the NPs size and composition. NPs size and concentration were maintained during analysis, as verified for NPs eluted from the LOV by particle tracking analysis (PTA). Moreover, concentration measurements for PEG–PLGA NPs loaded with an anti-inflammatory drug, methotrexate (MTX), after their incubation in simulated gastric and intestinal fluids were successfully achieved (recovery values of 102–115%, as confirmed by PTA), showing the suitability of the proposed method to support the development of polymeric NPs targeting intestinal delivery.

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Section: Experimental Sectionmentioning

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Lab-on-Valve Automated and Miniaturized Assessment of Nanoparticle Concentration Based on Light-Scattering

et al. 2023

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“…133,134 Moreover, lab-in-syringe and SIA systems were proposed for speciation analysis 135 and lab-on-valve sequential injection ELISA for the determination of carbamazepine. 136 The microfluidic platform still becoming important for developing new methods for environmental applications. Starting from 2015, Cogan et al 137 progressed a miniaturized microfluidic colorimetry for direct measurements of nitrate in natural water samples and wastewater.…”

Section: Application Of Flow-based System For Environmental Monitoringmentioning

confidence: 99%

Flow-based System: A Highly Efficient Tool Speeds Up Data Production and Improves Analytical Performance

et al. 2020

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In this review, we cite references from the period between 2015 and 2020 related to the use of a flow-based system as a tool to obtain a modern analytical system for speeding up data production and improving performance. Based on a great deal of concepts for automatic systems, there are several research groups introduced in the development of flow-based systems to increase sample throughput while retaining the reproducibility and repeatability as well as to propose new platforms of flow-based systems, such as microfluidic chip and paper-based devices. Additionally, to apply a developed system for on-site analysis is one of the key features for development. We believe that this review will be very interested and useful for readers because of its impact on developing novel analytical systems. The content of the review is categorized following their applications including quality control and food safety, clinical diagnostics, environmental monitoring and miscellaneous.

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“…Several analytical methods have been reported for the determination of carbamazepine in different matrices (e.g., serum, urine, dried blood spot (DBS), dried saliva spot, pharmaceuticals, and waste water). These methods included, for instance, spectrophotometry, high-performance thin-layer chromatography (HPTLC), high-performance liquid chromatography (HPLC), HPLC-MS/MS, near-infrared spectroscopy (NIR), electrochemical sensor, and enzyme-linked immunosorbent assay (ELISA) [4][5][6][7][8][9][10][11][12][13]. Moreover, due to its simplicity, speed, high efficiency, and low sample/reagent consumption, CE has also been applied for the determination of carbamazepine in tablets [14], serum, and plasma [3,[14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Paper-based molecularly imprinted-interpenetrating polymer network for on-spot collection and microextraction of dried blood spots for capillary electrophoresis determination of carbamazepine

2020

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Carbamazepine is an antiepileptic drug with a narrow therapeutic index, which requires an efficient method for blood level monitoring. Finger-prick dried blood spot (DBS) collection is an alternative microsampling technique, which is less invasive than conventional venipuncture. Paper-based molecularly imprinted-interpenetrating polymer networks (MI-IPN) were developed as blood collection devices, which allowed for selective on-spot microextraction of carbamazepine from DBS. A hybrid of homogeneous polystyrene and silica gel polymer was synthesized and coated on a Whatman ® Grade 1 filter paper. Proteins and other interferences in the blood samples were eliminated by using the MI-IPN collection devices, and the resulting DBS extracts were suitable for direct injection into the capillary electrophoretic instrument. The lower limit of quantitation of 4 μg/mL in capillary blood was achieved by the sweeping-micellar electrokinetic chromatography method using a KCl-containing matrix, which was sufficient for the therapeutic drug monitoring purposes. Method accuracies were in the range of 88.4 ± 4.5% to 94.5 ± 2.7% with RSD values ≤ 5.1%. The developed paper-based MI-IPN provided superior extraction efficiencies (92.2 ± 2.5%) in comparison with commercially available DBS collection cards, i.e., Whatman ® 903 protein saver card (59.8 ± 2.8%) and GenCollect™ 2.0 card (47.2 ± 1.4%). The paper-based MI-IPN devices for DBS collection and on-spot extraction were characterized by simple fabrication, low costs, disposability, and reduction in sample preparation steps, and their further developments might open new perspectives in clinical applications, such as in therapeutic drug monitoring.

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Automated lab-on-valve sequential injection ELISA for determination of carbamazepine

Cited by 13 publications

References 40 publications

Lab-on-Valve Automated and Miniaturized Assessment of Nanoparticle Concentration Based on Light-Scattering

Lab-on-Valve Automated and Miniaturized Assessment of Nanoparticle Concentration Based on Light-Scattering

Flow-based System: A Highly Efficient Tool Speeds Up Data Production and Improves Analytical Performance

Paper-based molecularly imprinted-interpenetrating polymer network for on-spot collection and microextraction of dried blood spots for capillary electrophoresis determination of carbamazepine

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