“…During IMS analysis, pre-selected positions create an array of points separated from each other at a determined distance and a mass spectrum is obtained at each spot. 6,7) With the development of automated instruments 8,9) and powerful so ware, 10,11) capable of rapidly acquiring and processing orderly yet massive imaging spectral data, it is now possible to simultaneously visualise innumerable individual biomolecules in tissue sections. 7,10) e identi cation and localisation of compounds such as neuropeptides, 12,13) neurotransmitters, 14) and small molecules 2) are especially important for the study of the brain, as it is a complex organ with a high spatial and somatotopic organisation.…”