Automation and Remote Synchrotron Data Collection

Gilski, Mirosław

doi:10.12693/aphyspola.114.331

Cited by 3 publications

(1 citation statement)

References 14 publications

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“…Full automation of the diffraction experiment is another consequence of the SR-related revolution. Today, remote SR diffraction experiments ("Fedex crystallography") are quickly gaining popularity (Gilski, 2008).…”

Section: Introduction Of Synchrotron Radiationmentioning

confidence: 99%

Personal remarks on the future of protein crystallography and structural biology.

Jaskólski¹

2010

Acta Biochim Pol

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Protein crystallography, the main experimental method of structural biology, has undergone in the recent past three revolutionary changes leading to its unexpected renaissance. They were connected with (i) the introduction of synchrotron radiation sources for X-ray diffraction experiments, (ii) implementation of Se-Met multiwavelength anomalous diffraction (MAD) for phasing, and (iii) initiation of structural genomics (SG) programs. It can be foreseen that in the next 10-15 years protein crystallography will continue to be in this revolutionary phase. We can expect not only an avalanche of protein crystal structures from SG centers, but also attacking of more demanding projects, such as the structure of membrane proteins and of very large macromolecular complexes. On the technological front, the introduction of X-ray radiation from free-electron lasers will revolutionize the experimental possibilities, making feasible even the imaging of single molecules and of intact biological cells.

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Section: Introduction Of Synchrotron Radiationmentioning

confidence: 99%

Personal remarks on the future of protein crystallography and structural biology.

Jaskólski¹

2010

Acta Biochim Pol

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Machine learning applications in macromolecular X-ray crystallography

Vollmar

Evans

2021

Crystallography Reviews

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Data Processing Programs for Analysis of Diffraction Images of Macromolecular Crystals Recorded using Synchrotron Radiation

Gilski

2012

Acta Phys. Pol. A

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Besides the crystal preparation, the rst and crucial step in the process of protein structure determination is proper processing of the collected diraction images, as they provide the experimental observations used throughout the entire process of structure solution and renement. In the last two decades several computer programs have been developed. Among the most used and popular are: HKL2000, MOSFLM, d*TREK and XDS package. To nd out the advantages and disadvantages of the data processing programs, several very dierent data sets, including diraction data from DNA/RNA and protein crystals were tested. It has been found that all the major programs for processing and analysis of diraction data give excellent and comparable results with good quality, medium resolution data sets, but their treatment of very high resolution or imperfect data diers in terms of indexing, spot integration, scaling and the treatment of errors. If the diraction data are of good quality and the problem is relatively straightforward, the automated approach to data processing may be most appropriate. On the other hand, if one is trying to squeeze out as much information from the experimental data as possible, then only expert manual processing can be successful, regardless of the data quality.

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Automation and Remote Synchrotron Data Collection

Cited by 3 publications

References 14 publications

Personal remarks on the future of protein crystallography and structural biology.

Personal remarks on the future of protein crystallography and structural biology.

Machine learning applications in macromolecular X-ray crystallography

Data Processing Programs for Analysis of Diffraction Images of Macromolecular Crystals Recorded using Synchrotron Radiation

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