Autonomous Detection of Aerosolized Biological Agents by Multiplexed Immunoassay with Polymerase Chain Reaction Confirmation

Hindson, Benjamin J.; McBride, Mary T.; Makarewicz, Anthony J.; Henderer, Bruce D.; Setlur, Ujwal S.; Smith, Sally M.; Gutierrez, Dora M.; Metz, Thomas; Nasarabadi, Shanavaz; Venkateswaran, K.; Farrow, Stephen W.; Colston, Bill W.; Dzenitis, John M.

doi:10.1021/ac0489014

Cited by 74 publications

(37 citation statements)

References 7 publications

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“…Immunoassays employing bead-based, suspension arrays and the Luminex system or other flow cytometer platforms have been established for the detection of Y. pestis, B. anthracis and botulinum toxin. A Luminex flow cytometer operates as the detector in the autonomous pathogen detection system (APDS), developed at Lawrence Livermore National Laboratory for detection of BWAs [55][56][57] . APDS, Luminex system performs immunoassay for initial detection of multiple BWAs and a positive detection event is confirmed by a multiplexed PCR-based microsphere microarray assay.…”

Section: Flow Cytometrymentioning

confidence: 99%

Biological Warfare Agents and their Detection and Monitoring Techniques (Review Paper)

Pal¹,

Sharma²,

Sharma³

et al. 2016

Def. Sc. Jl.

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Recently, threat from biological warfare agents (BWAs) has emerged as the foremost national and global security challenge because of their simple and cheap production, easy dispersal, complicated detection, expensive protection and psychological, economical and social impact. Early detection and identification of BWAs during intentional biological event is essential to initiate corrective emergency responses for management of such incidents. Efforts are being made across the globe for development of state of the art technologies and systems for detection and identification of BWAs. However, till date there is no single system which can detect all the bio-threat agents. In the present review, we describe the currently available techniques and systems for detection and identification of these agents. The basic identification techniques including biological culture, immunological methods, nucleic acid based detection, MALDI-TOF MS, cellular fatty acid profiling and flow cytometry based detection are presented. Detection of BWAs with bio-sensors, surface plasmon resonance, biological detectors, and stand-off detection systems is also summarized. However, despite of availability of several techniques and tools, no full proof system is available for detection/identification of all the BWAs.

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Section: Flow Cytometrymentioning

confidence: 99%

Biological Warfare Agents and their Detection and Monitoring Techniques (Review Paper)

Pal¹,

Sharma²,

Sharma³

et al. 2016

Def. Sc. Jl.

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“…Autonomous pathogen detection system 40,41 (APDS), was developed at lawrence livermore national laboratory for continuous sampling of the air for airborne pathogens and toxins. The system can run unattended for 1 week.…”

Section: Integrated Detection Systemmentioning

confidence: 99%

Systems for Detection and Identification of Biological Aerosols (Review Paper)

Švábenská¹

2012

DSJ

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Easy and inexpensive manufacturing of biological weapons, their complicated detection, expensive protection, difficult treating of affected individuals, selective impact only for people, animals or plants, are all factors making an effective defense against biological warfare agents very difficult. The aim of this study is an introduction to the systems for the detection and identification of biological aerosols containing dangerous bioagents. The basic techniques used for detection and identification of bioagents are described, including physical, molecular, immunochemical, and other ligand assays. Measuring systems and equipment for the individual techniques are summarised.

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“…The APDS has been used to detect the threat agent simulants Bacillus globigii, Erwinia herbicola, MS2, and ovalbumin singly and in mixtures (196) and to simultaneously detect of Bacillus anthracis and Yersinia pestis in air (197). Researchers have recently reported a successful integration of PCR with the APDS to produce a completely automated system that will automatically perform confirmatory PCR on any sample that produces a positive immunoassay result (124). During aerosol chamber tests with Bacillus anthracis, Yersinia pestis, Bacillus globigii, and botulinum toxoid, all agents were detected using the automated immunoassay procedure, and the presence of the three bacteria was confirmed using the automated PCR process.…”

Section: Immunological Detectionmentioning

confidence: 99%

Current and Developing Technologies for Monitoring Agents of Bioterrorism and Biowarfare

et al. 2005

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SUMMARY Recent events have made public health officials acutely aware of the importance of rapidly and accurately detecting acts of bioterrorism. Because bioterrorism is difficult to predict or prevent, reliable platforms to rapidly detect and identify biothreat agents are important to minimize the spread of these agents and to protect the public health. These platforms must not only be sensitive and specific, but must also be able to accurately detect a variety of pathogens, including modified or previously uncharacterized agents, directly from complex sample matrices. Various commercial tests utilizing biochemical, immunological, nucleic acid, and bioluminescence procedures are currently available to identify biological threat agents. Newer tests have also been developed to identify such agents using aptamers, biochips, evanescent wave biosensors, cantilevers, living cells, and other innovative technologies. This review describes these current and developing technologies and considers challenges to rapid, accurate detection of biothreat agents. Although there is no ideal platform, many of these technologies have proved invaluable for the detection and identification of biothreat agents.

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Autonomous Detection of Aerosolized Biological Agents by Multiplexed Immunoassay with Polymerase Chain Reaction Confirmation

Cited by 74 publications

References 7 publications

Biological Warfare Agents and their Detection and Monitoring Techniques (Review Paper)

Biological Warfare Agents and their Detection and Monitoring Techniques (Review Paper)

Systems for Detection and Identification of Biological Aerosols (Review Paper)

Current and Developing Technologies for Monitoring Agents of Bioterrorism and Biowarfare

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