Autonomous system for rapid field quantification of Escherichia coli in surface waters

Abstract

Rapid quantification of bacterial pollution is a requirement in water quality applications ranging from recreational water use, agriculture and aquaculture to drinking and wastewater treatment. The method and instruments presented in this work should enable fast and accurate bacterial concentration measurements to be performed in a portable or in situ manner, thus simplifying operational logistics, reducing time-to-result delays, and eliminating sample transportation constraints associated with traditional techniques.

Capacity challenges in water quality monitoring: understanding the role of human development

“…Future research may also deepen our analyses, by further including questions of data management, assessment and interpretation of data, or by considering different and emerging methodologies for monitoring (e.g. Angelescu et al 2019;Rérolle et al 2019). Also, the inclusion of qualitative data on the challenges of water quality monitoring may help to better understand the mechanisms beyond such negative relations between HDI and the application of water quality parameters.…”

Monitoring Approaches for Faecal Indicator Bacteria in Water: Visioning a Remote Real-Time Sensor for E. coli and Enterococci

“…A laboratory-in-vial water monitoring system is a type of biosensor calibrated to detect target indicators, including E. coli and enterococci, with minimal human interaction. With in situ or portable capability, it generally processes samples within its compartments by applying specific bioreagents to samples and, after incubation, measurements made through fluorescence or other optical devices over a specific time frame and/or measurement interval [19,104,105].…”

“…It can be initiated through either on-demand sampling from cell phone messages or a web-based control page. The measurements can be preprogrammed, and external trigger turbidity and conductivity probes can be established to trigger automatic sampling and measurement operations [19]. Automatic sampling is made through the ALERT System's internal vacuum sampling module [106], and consists of seven individual bioreactors, each containing a 25 mL sampling vial and sampling adapter [19].…”

“…The measurements can be preprogrammed, and external trigger turbidity and conductivity probes can be established to trigger automatic sampling and measurement operations [19]. Automatic sampling is made through the ALERT System's internal vacuum sampling module [106], and consists of seven individual bioreactors, each containing a 25 mL sampling vial and sampling adapter [19]. After taking all seven individual measurements, a less than 30 min maintenance period is required, which consists of changing the vials, adding fresh reagent to decontaminate the sample tubing and replacing the battery [19].…”

“…Automatic sampling is made through the ALERT System's internal vacuum sampling module [106], and consists of seven individual bioreactors, each containing a 25 mL sampling vial and sampling adapter [19]. After taking all seven individual measurements, a less than 30 min maintenance period is required, which consists of changing the vials, adding fresh reagent to decontaminate the sample tubing and replacing the battery [19]. When the sample in an individual bioreactor is mixed with the specific bioreagent and growth medium, it is incubated.…”