Autonomously driving multiplexed hierarchical hybridization chain reaction of a DNA cobweb sensor for monitoring intracellular microRNA

“…To improve the sensitivity of single-stage HCR, the cascaded HCR reaction was designed. [35][36] Wei et al constructed a cascaded HCR signal amplification circuit for miRNA-21 detection. [37] As shown in Figure 2E, the circuit is composed of two parts: upstream HCR-1 and downstream HCR-2.…”

Section: Hybridization Chain Reaction (Hcr)-based Biosensorsmentioning

confidence: 99%

Integration of Isothermal Enzyme‐Free Nucleic Acid Circuits for High‐Performance Biosensing Applications

Wang,

Shang,

Zhao

et al. 2023

ChemPlusChem

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The isothermal enzyme‐free nucleic acid amplification method plays an indispensable role in biosensing by virtue of its simple, robust, and highly efficient properties without the assistance of temperature cycling or/and enzymatic biocatalysis. Until to now, enzyme‐free nucleic acid amplification has been extensively utilized for biological assays and has achieved the highly sensitive detection of various biological targets, including DNAs, RNAs, small molecules, proteins, and even cells. In this review, the mechanisms of entropy‐driven reaction, hybridization chain reaction, catalytic hairpin assembly and DNAzyme were concisely described and their recent application as biosensors was comprehensively summarized. Furthermore, the current problems and the developments of these DNA circuits were also discussed.

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“…However, the sensitivity of DNA nanotweezers is not sufficient to meet the diagnostic requirements. Signal amplification techniques such as a hybridization chain reaction and rolling circle amplification are not ideal because they need a continuous input of either fuel strands or enzymes. ,− Among all different designs of DNA nanostructures, a two-dimensional DNA tile is able to self-repeat into a large-scale structure. − We designed nine oligomers that self-assemble into a four-arm DNA junction . Two individual tiles, named TA and TB (Figure S1), were adopted in this study .…”

Section: Introductionmentioning

confidence: 99%

Self-Assembly of DNA Tiles with G-Quadruplex DNAzyme Catalytic Activity for Sensing Applications

Wang

Cui

Tanner

et al. 2022

ACS Appl. Bio Mater.

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DNA tiles form through self-assembly of a small number of DNA strands that interact through basic repeated interactions, allowing the growth of nanoscale structures seeded by molecular inputs. If an approach for catalytic signal amplification can be integrated into the resultant nanostructure, then one can anticipate biosensing or diagnostic applications mediated by DNA tile self-assembly. Here, two-dimensional DNA tiles with split quadruplexes were designed as diagnostic tools for nucleic acid sensing without the use of protein enzymes. The presence of a target sequence leads to formation of extended microscale structures with arrayed multiple G-quadruplexes across the tile plane, with catalytic activity coupled to a colorimetric reporter. Such a mechanism has potential for low-cost signal amplification using unmodified DNA without the use of protein enzymes for biosensing.

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Autonomously driving multiplexed hierarchical hybridization chain reaction of a DNA cobweb sensor for monitoring intracellular microRNA

Cited by 15 publications

References 22 publications

Chemiluminescence resonance energy transfer-based multistage nucleic acid amplification circuits for MiRNA detection with low background

Chemiluminescence resonance energy transfer-based multistage nucleic acid amplification circuits for MiRNA detection with low background

Integration of Isothermal Enzyme‐Free Nucleic Acid Circuits for High‐Performance Biosensing Applications

Self-Assembly of DNA Tiles with G-Quadruplex DNAzyme Catalytic Activity for Sensing Applications

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