Autophagy: a molecular switch to regulate adipogenesis and lipolysis

Sekar, Mouliganesh; Thirumurugan, Kavitha

doi:10.1007/s11010-021-04324-w

Cited by 22 publications

(14 citation statements)

References 131 publications

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“…Previous studies showed that autophagy is necessary for osteoblast differentiation. , Interestingly, many small GTPases are known to regulate autophagy. For instance, RAB27B and RAB33A were documented to promote and inhibit autophagy, respectively. − In line with this notion, our MRM results revealed up- and downregulations of RAB27B and RAB33A during osteogenesis, respectively (Figure A,B), which were further confirmed by Western blot analysis (Figure C,D).…”

Section: Resultssupporting

confidence: 79%

Targeted Proteomic Profiling Revealed Roles of Small GTPases during Osteogenic Differentiation

et al. 2023

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The small GTPase superfamily of proteins are crucial for numerous cellular processes, including early development. The roles of these proteins in osteogenic differentiation, however, remained poorly explored. In this study, we employed a high-throughput targeted proteomic method, relying on scheduled liquid chromatography−multiple-reaction monitoring (LC-MRM) coupled with synthetic stable isotope-labeled peptides, to interrogate systematically the temporal responses of the entire small GTPase proteome during the course of osteogenic differentiation of H9 human embryonic stem cells. Our results demonstrated that the method offers high quantification accuracy, reproducibility, and throughput. In addition, the quantification results revealed altered expression of a large number of small GTPases accompanied with osteogenic differentiation, especially those involved with autophagy. We also documented a previously unrecognized role of KRAS in osteogenesis, where it regulates the accumulation of extracellular matrix for mineralization through attenuating the activity of secreted matrix metalloproteinase 9 (MMP9). Together, this study represents a novel application of a state-of-the-art analytical method, i.e., targeted quantitative proteomics, for revealing the progressive reprogramming of the small GTPase proteome during osteogenic differentiation of human embryonic stem cells, and our results revealed KRAS as a new regulator for osteogenesis.

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Section: Resultssupporting

confidence: 79%

Targeted Proteomic Profiling Revealed Roles of Small GTPases during Osteogenic Differentiation

et al. 2023

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“…The interconnection of glycolysis and the TCA cycle is visualized in ( Figure 2 A,B). Fatty acids (FAs) are the most common form of stored energy, and triacylglycerols (TAGs) are the most common non-toxic form of FAs [ 45 ]. Generally, there are four sources from which to acquire FAs and TAGs: de novo lipogenesis (DNL), cytoplasmic TAG stores, FAs derived from TAGs of lipoprotein remnants taken up by the liver, and plasma non-esterified fatty acids (NEFA) released by adipose tissue [ 46 ].…”

Section: Introductionmentioning

confidence: 99%

Regulation of Autophagy via Carbohydrate and Lipid Metabolism in Cancer

Alizadeh

Kavoosi

Singh

et al. 2023

Cancers

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Metabolic changes are an important component of tumor cell progression. Tumor cells adapt to environmental stresses via changes to carbohydrate and lipid metabolism. Autophagy, a physiological process in mammalian cells that digests damaged organelles and misfolded proteins via lysosomal degradation, is closely associated with metabolism in mammalian cells, acting as a meter of cellular ATP levels. In this review, we discuss the changes in glycolytic and lipid biosynthetic pathways in mammalian cells and their impact on carcinogenesis via the autophagy pathway. In addition, we discuss the impact of these metabolic pathways on autophagy in lung cancer.

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“…The interconnection of glycolysis and the TCA cycle is visualized in (Figure 2A&B). Fatty acids (FAs) are the most common form of stored energy, and triacylglycerols (TAGs) are the most common non-toxic form of FAs [44]. Generally, there are four sources to acquire FAs and TAGs: de novo lipogenesis (DNL), cytoplasmic TAG stores, FAs derived from TAGs of lipoprotein remnants taken up by the liver, and plasma non-esterified fatty acids (NEFA) released by adipose tissue [45].…”

Section: Introductionmentioning

confidence: 99%

Regulation of Autophagy via Carbohydrate and Lipid Metabolism in Cancer

Alizadeh¹,

Kavoosi²,

Singh³

et al. 2023

Preprint

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Metabolic changes are an important component of tumor cell progression. Tumor cells adapt to environmental stresses via changes to carbohydrate and lipid metabolism. Autophagy, a physiological process in mammalian cells that digests damaged organelles and misfolded proteins via lysosomal degradation is closely associated with metabolism in mammalian cells acting as a meter of cellular ATP levels. In this review, we discuss the changes in glycolytic and lipid biosynthetic pathways in mammalian cells and their impact on carcinogenesis via the autophagy pathway. Also, we discuss the impact of these metabolic pathways on autophagy in lung cancer.

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Autophagy: a molecular switch to regulate adipogenesis and lipolysis

Cited by 22 publications

References 131 publications

Targeted Proteomic Profiling Revealed Roles of Small GTPases during Osteogenic Differentiation

Targeted Proteomic Profiling Revealed Roles of Small GTPases during Osteogenic Differentiation

Regulation of Autophagy via Carbohydrate and Lipid Metabolism in Cancer

Regulation of Autophagy via Carbohydrate and Lipid Metabolism in Cancer

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