Autoradiographic distribution of vasoactive intestinal polypeptide receptors in rabbit and rat small intestine

Sayadi, Hassan; Harmon, John W.; Moody, Terry W.; Korman, Louis Y.

doi:10.1016/0196-9781(88)90005-8

Cited by 18 publications

(9 citation statements)

References 17 publications

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“…Surprisingly although VPAC 1 R has been detected in the gut of human, rat and goldfish (Sreedharan et al 1993, Usdin et al 1994, Chow 1997 it was absent in Fugu. This may merely reflect the position of the sample of Fugu gut collected, as VIP receptors have a differential distribution in this tissue (Sayadi et al 1988, Korman et al 1989. It should be noted that further overlap of tissue expression may occur between these six receptors, as the RT-PCRs were only carried out on single tissues from juvenile fish and developmental differences may also occur.…”

Section: Discussionmentioning

confidence: 99%

Duplicated receptors for VIP and PACAP (VPAC1R and PAC1R) in a teleost fish, Fugu rubripes

Cardoso¹,

Power²,

Elgar³

et al. 2004

Journal of Molecular Endocrinology

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Two principal groups of receptors orthologous with human PAC 1 R and VPAC 1 R and were identified and characterised at the genomic level in the teleost fish Fugu rubripes. An additional group orthologous with VPAC 2 R was also identified and partially characterised. In Fugu, gene duplication of each of the PAC 1 Rs, VPAC 1 Rs and VPAC 2 Rs appears to have occurred. The topology of the tree surrounding the Fugu duplications and other isolated piscine sequences indicates that the duplication events for these six genes clearly preceded the speciation event leading to the Cypriniformes and Tetraodontiformes and is probably teleost-specific. Overall, the combined pattern of gene expression for each pair of duplicated genes mirrored the expression in other vertebrates. However, within each pair of duplicates further specialisation had occurred, with each demonstrating differential tissue distribution profiles suggesting they that may be responsible for the divergent action of the ligands, vasoactive intestinal peptide (VIP) and pituitary adenylate cyclase-activating peptide (PACAP). The Fugu VPAC 1 R gene regions showed conserved synteny with human chromosome 3p21·3 and also C. elegans chromosome X, indicating that the putative ancestral human chromosome 3 region may be equivalent to chromosome X in

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Section: Discussionmentioning

confidence: 99%

Duplicated receptors for VIP and PACAP (VPAC1R and PAC1R) in a teleost fish, Fugu rubripes

Cardoso¹,

Power²,

Elgar³

et al. 2004

Journal of Molecular Endocrinology

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“…12 A high concentration of VIP receptors is found in the mucosa of the gut, above all in the small intestine, in both humans and animals. [13][14][15][16][17][18] Interestingly, exposure of pigs to irradiation leads to modifications in VIP receptor characteristics in isolated membranes from the jejunum of these animals. 11 These effects were observed at doses lower than those normally associated with the manifestation of marked gastrointestinal dysfunction.…”

mentioning

confidence: 99%

Binding Sites for VIP in the Reorganizing Mucosa of the Irradiated Bowel^a

Höckerfelt

Hansson

Gulbenkian

et al. 1998

Annals of the New York Academy of Sciences

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Rats were given radiotherapy (total dose 30 Gy) over the abdomen. Seven days later specimens of the duodenum were prepared for in vitro receptor autoradiography using the radioligand [125I]VIP. The autoradiograms were quantitatively analyzed using a computer system. Histological examination revealed that a very marked reorganization of the mucosa had occurred in response to irradiation. Using receptor autoradiography, we found [125I]VIP-specific binding sites in the reorganizing mucosa, except where denudation had occurred. Such binding sites also occurred in the smooth muscle layer of the duodenal wall. The observations suggest that VIP has profound effects in radiation-induced enteropathy.

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“…These findings suggest that VIP may exert a direct inhibitory effect on motor activity via VIP receptors located on intestinal smooth muscle, rather than via cholinergic receptors. The presence of VIP receptors on smooth muscle appears to be a possibility, since binding of radiolabelled VIP to smooth muscle cells 16 and expression of VIP receptor mRNA in smooth muscle 17 have been reported.…”

Section: Discussionmentioning

confidence: 99%

Motor activity of vascularly perfused rat duodenum. 2. Effects of VIP, PACAP27 and PACAP38

Yamamoto

Kuwahara

Fujimura

et al. 1999

Neurogastroenterology Motil

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We examined the mechanisms of effects of vasoactive intestinal polypeptide (VIP), pituitary adenylate cyclase activating polypeptide (PACAP) 27 and PACAP38 on spontaneously occurring pressure waves in ex vivo perfused rat duodenum. VIP and PACAPs dose-dependently reduced the percentage motor index of pressure waves; this reduction was not prevented by atropine, hexamethonium or tetrodotoxin (TTX). VIP and PACAPs abolished acetylcholine-induced stimulation of pressure waves, even in the presence of TTX. These findings suggest that VIP and PACAPs may exert direct inhibitory effects via VIP/PACAP receptors located on smooth muscle rather than via cholinergic receptors. The inhibitory effects of VIP and PACAPs were partially antagonized by the VIP receptor antagonists VIP(10-28), suggesting that VIP and PACAPs share common receptor sites on intestinal smooth muscle. The effects of VIP and PACAPs were completely antagonized by nitric oxide (NO) synthase inhibitor NG-nitro-L-arginine (L-NA), suggesting that NO mediates the inhibitory effects of VIP and PACAPs on duodenal motility. Furthermore, single injection of L-NA stimulated spontaneously occurring pressure waves, while VIP(10-28) did not affect them. These findings suggest that VIP/PACAPs and NO strongly interact as an inhibitory mediator on duodenal motility, but that their modes of action in doing so may differ.

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Autoradiographic distribution of vasoactive intestinal polypeptide receptors in rabbit and rat small intestine

Cited by 18 publications

References 17 publications

Duplicated receptors for VIP and PACAP (VPAC1R and PAC1R) in a teleost fish, Fugu rubripes

Duplicated receptors for VIP and PACAP (VPAC1R and PAC1R) in a teleost fish, Fugu rubripes

Binding Sites for VIP in the Reorganizing Mucosa of the Irradiated Bowel^a

Motor activity of vascularly perfused rat duodenum. 2. Effects of VIP, PACAP27 and PACAP38

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Autoradiographic distribution of vasoactive intestinal polypeptide receptors in rabbit and rat small intestine

Cited by 18 publications

References 17 publications

Duplicated receptors for VIP and PACAP (VPAC1R and PAC1R) in a teleost fish, Fugu rubripes

Duplicated receptors for VIP and PACAP (VPAC1R and PAC1R) in a teleost fish, Fugu rubripes

Binding Sites for VIP in the Reorganizing Mucosa of the Irradiated Bowela

Motor activity of vascularly perfused rat duodenum. 2. Effects of VIP, PACAP27 and PACAP38

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Binding Sites for VIP in the Reorganizing Mucosa of the Irradiated Bowel^a