Auxin acts as a downstream signaling molecule involved in hydrogen sulfide-induced chilling tolerance in cucumber

Zhang, Xiaowei; Liu, Fengjiao; Zhai, Jing; Li, Fude; Bi, Huangai; Ai, Xizhen

doi:10.1007/s00425-020-03362-w

Cited by 64 publications

(52 citation statements)

References 62 publications

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“…In a previous study, we demonstrated that NaHS could improve the cold tolerance of cucumber seedlings in a concentration-dependent manner, and 1.0 mM NaHS treatment showed a very significant difference compared with the control (Zhang et al ., 2020). Here, we found that 1.0 mM NaHS significantly reduced cold stress injury, accumulation of H 2 O 2 and O 2 .- , as well as EL in cucumber seedlings after exposure to 5°C for 48 h. However, 0.15 mM H 2 S scavenger HT increased cold stress injury, H 2 O 2 , O 2 .- and EL, compare with the deionized water (H 2 O, as a comparison)-treated seedlings (Fig.…”

Section: Resultsmentioning

confidence: 93%

“…IAA content was measured using high-performance liquid chromatography-triple quadrupole mass spectrometry (HPLC–MS, Thermo Fisher Scientific, TSQ Quantum Access) according to the method of Li et al (2014) with minor modifications by Zhang et al (2020). In brief, leaf samples were extracted with 80% methanol (containing 30 μg.ml −1 sodium diethyldithiocarbamate) and the supernatant was retained rotary evaporation (Shanghai EYELA, N-1210B).…”

Section: Methodsmentioning

confidence: 99%

“…The activity of FMO was detected using an enzyme-linked immunosorbent assay (ELISA) kit (Jiangsu Meimian Industrial Co. Ltd, Jiangsu, China) as described by Zhang et al (2020).…”

Section: Methodsmentioning

confidence: 99%

“…No. 15200, Beijing, China), respectively as described by Galluzzi and Kroemer (2014) and modified by Zhang et al (2020). In brief, the samples were infiltrated in 20 μM H 2 O 2 fluorescent probe at 25 °C under dark conditions for 30 min.…”

Section: Methodsmentioning

confidence: 99%

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Hydrogen sulfide improves the cold stress resistance through the CsARF5-CsDREB3 module in cucumber

Zhang

et al. 2021

Preprint

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Hydrogen sulfide (H2S) plays a crucial role in regulating cold tolerance. But the synergistic regulation of H2S and auxin in the plant response to cold stress has not been reported. In the study, we found that sodium hydrosulfide (NaHS, an H2S donor) treatment enhanced the cold tolerance of cucumber seedlings and increased the level of auxin. CsARF5, a cucumber auxin response factor (ARF) gene was isolated and its role in regulating H2S-mediated cold stress tolerance was described. Transgenic cucumber leaves overexpressing CsARF5 were obtained. Physiological analysis indicated that overexpression of CsARF5 enhanced the cold stress tolerance of cucumber and the regulation of the cold stress response by CsARF5 depends on H2S. In addition, molecular assays showed that CsARF5 modulated cold stress response by directly activating the expression of the dehydration-responsive element-binding (DREB)/C-repeat binding factor (CBF) gene CsDREB3, which was identified as a positive regulator of cold stress. Taken together, our results suggest that CsARF5 plays an important role in H2S-mediated cold stress in cucumber. These results shed light on the molecular mechanism by which H2S regulates cold stress response by mediating auxin signaling, and will provide insights for further studies on the molecular mechanism by which H2S regulates cold stress.

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Section: Resultsmentioning

confidence: 93%

Section: Methodsmentioning

confidence: 99%

“…The activity of FMO was detected using an enzyme-linked immunosorbent assay (ELISA) kit (Jiangsu Meimian Industrial Co. Ltd, Jiangsu, China) as described by Zhang et al (2020).…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Hydrogen sulfide improves the cold stress resistance through the CsARF5-CsDREB3 module in cucumber

Zhang

et al. 2021

Preprint

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“…Application of NaHS significantly increased the activity of favin monooxygenase (FMO) and the relative expression of the FMO-like protein YUCCA2 in cucumber seedlings, which in turn increased the level of endogenous IAA and improved cold tolerance, seen as decreases in electrolyte leakage and accumulation of ROS and increases in expression of genes and enzyme activities related to photosynthesis. Application of IAA or removal of H 2 S had little effect on the signaling of the other molecule, but the IAA polar transport inhibitor NPA inhibited H 2 S-induced cold tolerance and defense gene expression [ 88 ]. IAA participates in H 2 S-induced stress tolerance in plants as a downstream signaling molecule, while H 2 S promotes auxin signal transduction by regulating the expression of auxin-related genes and the synthesis of auxin, thereby enhancing the plant tolerance to adverse environmental conditions.…”

Section: Mechanism By Which H 2 S Regulates Plamentioning

confidence: 99%

Crosstalk between Hydrogen Sulfide and Other Signal Molecules Regulates Plant Growth and Development

Xuan

Wang

et al. 2020

IJMS

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Hydrogen sulfide (H2S), once recognized only as a poisonous gas, is now considered the third endogenous gaseous transmitter, along with nitric oxide (NO) and carbon monoxide (CO). Multiple lines of emerging evidence suggest that H2S plays positive roles in plant growth and development when at appropriate concentrations, including seed germination, root development, photosynthesis, stomatal movement, and organ abscission under both normal and stress conditions. H2S influences these processes by altering gene expression and enzyme activities, as well as regulating the contents of some secondary metabolites. In its regulatory roles, H2S always interacts with either plant hormones, other gasotransmitters, or ionic signals, such as abscisic acid (ABA), ethylene, auxin, CO, NO, and Ca2+. Remarkably, H2S also contributes to the post-translational modification of proteins to affect protein activities, structures, and sub-cellular localization. Here, we review the functions of H2S at different stages of plant development, focusing on the S-sulfhydration of proteins mediated by H2S and the crosstalk between H2S and other signaling molecules.

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Roles of Hydrogen Sulfide in Regulating Temperature Stress Response in Plants

Banerjee

Roychoudhury

2021

Plant Growth Regulators

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Auxin acts as a downstream signaling molecule involved in hydrogen sulfide-induced chilling tolerance in cucumber

Cited by 64 publications

References 62 publications

Hydrogen sulfide improves the cold stress resistance through the CsARF5-CsDREB3 module in cucumber

Hydrogen sulfide improves the cold stress resistance through the CsARF5-CsDREB3 module in cucumber

Crosstalk between Hydrogen Sulfide and Other Signal Molecules Regulates Plant Growth and Development

Roles of Hydrogen Sulfide in Regulating Temperature Stress Response in Plants

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