AUXIN RESPONSE FACTOR protein accumulation and function

Abstract

Auxin is a key regulator of plant developmental processes. Its effects on transcription are mediated by the AUXIN RESPONSE FACTOR (ARF) family of transcription factors. ARFs tightly control specific auxin responses necessary for proper plant growth and development. Recent research has revealed that regulated ARF protein accumulation and ARF nucleo‐cytoplasmic partitioning can determine auxin transcriptional outputs. In this review, we explore these recent findings and consider the potential for regulated ARF accumulation in driving auxin responses in plants.

Comparative mutant analyses reveal a novel mechanism of ARF regulation in land plants

“…DNA encoding 4 GS repeats, a porcine teschovirus-1 2A (P2A) cleavage peptide ( 6 ), and mScarlet3 were synthesized using TWIST biosciences fragment synthesis. The P2A- mScarlet3 insert was amplified by PCR using Q5 2× master mix (NEB).…”

“…Canonical auxin signaling occurs through the activity of a suite of deeply conserved transcriptional regulators found in all land plants ( 3 , 5 , 6 ). The AUXIN/INDOLE-3-ACETIC- ACID repressors (AUX/IAAs), AUXIN RESPONSE FACTORS (ARFs) and the TRANSPORT INHIBITOR RESPONSE 1 /AUXIN-SIGNALING F-BOX (TIR1/AFB) co-receptors are components of the best-characterized pathway of auxin-mediated transcriptional regulation (reviewed by ( 7–9 )).…”