In this study, it is aimed to investigate the effect of the online science course, which is carried out by using the virtual museums created with the web 2.0 tool, on the academic achievement and science attitudes of the fifth-grade students. In addition, student opinions about the virtual museum were also taken within the scope of the study. The research was conducted based on mixed method research in which qualitative and quantitative research models were combined. Quasi-experimental design with pre-test and post-test control groups was used in the quantitative dimension. The study group consisted of a total of 100 fifth grade students studying at a public school in the academic year of 2020-2021. In the determination of the study group, the easily accessible case sampling method, which is one of the purposeful sampling methods, was used. The study group was divided into two, and science teaching was carried out in the experimental group with a virtual museum created with web 2.0 tools; in the control group, traditional science teaching was carried out by adhering to the activities in the textbook. In the qualitative aspect of the study, opinions about the virtual museum were taken from the students. In the study, the science lesson attitude scale and the academic achievement test prepared for the subject of human and environment were used as data collection tools. While the data obtained from the science lesson attitude scale and achievement test were analysed with the SPSS program; the interview data were analysed using descriptive analysis. As a result of the research, it was revealed that virtual museums, which are web 2.0 tools, have a positive effect on students' science lesson attitudes and academic achievement.