Avaliação do comportamento biológico de homoenxertos valvares pulmonares descelularizados: estudo experimental em ovinos

Navarro, Fábio Binhara; Costa, Francisco Diniz Affonso da; Mulinari, Leonardo; Pimentel, Gustavo Klug; Roderjan, João Gabriel; Vieira, Eduardo; Noronha, Lúcia de; Miyague, Nelson Itiro

doi:10.1590/s0102-76382010000300015

Cited by 21 publications

(8 citation statements)

References 25 publications

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“…Moreover, a recellularization, which is also of major concern for decellularized scaffolds integration and function, occurred and was progressive but still partial at 3 months. This phenomenon was also observed by others and a longer follow-up will investigate if complete recellularization can be achieved [ 39 ].…”

Section: Discussionsupporting

confidence: 71%

Porcine pulmonary valve decellularization with NaOH-based vs detergent process: preliminary in vitro and in vivo assessments

Steenberghe

Schubert

Gerelli

et al. 2018

J Cardiothorac Surg

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BackgroundGlutaraldehyde fixed xenogeneic heart valve prosthesis are hindered by calcification and lack of growth potential. The aim of tissue decellularization is to remove tissue antigenicity, avoiding the use of glutaraldehyde and improve valve integration with low inflammation and host cell recolonization. In this preliminary study, we investigated the efficacy of a NaOH-based process for decellularization and biocompatibility improvement of porcine pulmonary heart valves in comparison to a detergent-based process (SDS-SDC0, 5%).MethodsNative cryopreserved porcine pulmonary heart valves were treated with detergent and NaOH-based processes.Decellularization was assessed by Hematoxylin and eosin/DAPI/alpha-gal/SLA-I staining and DNA quantification of native and processed leaflets, walls and muscles.Elongation stress test investigated mechanical integrity of leaflets and walls (n = 3 tests/valve component) of valves in the native and treated groups (n = 4/group).Biochemical integrity (collagen/elastin/glycosaminoglycans content) of leaflet-wall and muscle of the valves (n = 4/group) was assessed and compared between groups with trichrome staining (Sirius Red/Miller/Alcian blue).Secondly, a preliminary in vivo study assessed biocompatibility (CD3 and CD68 immunostaining) and remodeling (Hematoxylin and eosin/CD31 and ASMA immunofluorescent staining) of NaOH processed valves implanted in orthotopic position in young Landrace pigs, at 1 (n = 1) and 3 months (n = 2).ResultsDecellularization was better achieved with the NaOH-based process (92% vs 69% DNA reduction in the wall). Both treatments did not significantly alter mechanical properties. The detergent-based process induced a significant loss of glycosaminoglycans (p < 0,05).In vivo, explanted valves exhibited normal morphology without any sign of graft dilatation, degeneration or rejection. Low inflammation was noticed at one and three months follow-up (1,8 +/− 3,03 and 0,9836 +/− 1,3605 CD3 cells/0,12 mm2 in the leaflets). In one animal, at three months we documented minimal calcification in the area of sinus leaflet and in one, microthrombi formation on the leaflet surface at 1 month. The endoluminal side of the valves showed partial reendothelialization.ConclusionsNaOH-based process offers better porcine pulmonary valve decellularization than the detergent process. In vivo, the NaOH processed valves showed low inflammatory response at 3 months and partial recellularization. Regarding additional property of securing, this treatment should be considered for the new generation of heart valves prosthesis.Graphical abstractGraphical abstract of the study

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Section: Discussionsupporting

confidence: 71%

Porcine pulmonary valve decellularization with NaOH-based vs detergent process: preliminary in vitro and in vivo assessments

Steenberghe

Schubert

Gerelli

et al. 2018

J Cardiothorac Surg

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“…They conclude that interobserver and intraobserver agreement in the diagnosis of ILDs based on HRCT scans ranged from fair to almost perfect and was influenced by radiologist expertise, clinical history and confidence level. Navarro et al101 evaluated the biological and functional behavior of decellularized pulmonary homografts (Decell-H), treated by a sodium dodecil sulfate solution (0.1%) and found that in their experimental model, the Decell-H behaved as an excellent valve substitute. Pego-Fernandes et al102 report on the functional evaluation experience with ex vivo perfusion of twelve donor lungs deemed unacceptable in Sao Paulo, Brazil.…”

Section: Introductionmentioning

confidence: 99%

A survey of recently published cardiovascular, hematological and pneumological original articles in the Brazilian scientific press

Patel¹,

Caramelli²,

Gomes³

2011

Clinics

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Recent original scientific contributions published in selected Brazilian periodicals and classifiable under cardiovascular and pulmonary subject categories cover a wide range of sub specialties, both clinical and exprimental. Because they appear in journals with only recently enhanced visibility, we have decided to highlight a number of specific items appeared in four Brazilian journals, because we understand that this is an important subsidy to keep our readership adequately informed. These papers cover extensive sub-areas in both fields.

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“…The original search by Uiterwijk et al 20 identified 817 articles, of which 80 were included in qualitative analysis for this study, after reassessment of studies that reported on additional data from previously reported animals ( Supplemental Figure 1 ). All 80 studies 37 , 39 , 40 , 41 , 42 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 , 75 , 76 , 77 , 78 , 79 , 80 , 81 , 82 , 83 , 84 , 85 , 86 , 87 , 88 , 89 , 90 , 91 , 92 , 93 , 94 , 95 , 96 , 97 , 98 , 99 , 100 , 101 ,…”

Section: Resultsmentioning

confidence: 99%

Calcification in Pulmonary Heart Valve Tissue Engineering

Valk

Fomina

Uiterwijk

et al. 2023

JACC: Basic to Translational Science

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Avaliação do comportamento biológico de homoenxertos valvares pulmonares descelularizados: estudo experimental em ovinos

Cited by 21 publications

References 25 publications

Porcine pulmonary valve decellularization with NaOH-based vs detergent process: preliminary in vitro and in vivo assessments

Porcine pulmonary valve decellularization with NaOH-based vs detergent process: preliminary in vitro and in vivo assessments

A survey of recently published cardiovascular, hematological and pneumological original articles in the Brazilian scientific press

Calcification in Pulmonary Heart Valve Tissue Engineering

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