Avermectin stress varied structure and function of gut microbial community in Lymantria dispar asiatica (Lepidoptera: Lymantriidae) larvae

Zeng, Jianyong; Vuong, Thi-Minh-Dien; Shi, Jiang‐Hong; Shi, Zhang; Guo, Jiaxing; Zhang, Guocai; Bing, Bi

doi:10.1016/j.pestbp.2020.01.013

Cited by 22 publications

(11 citation statements)

References 35 publications

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“…The present studies showed that Firmicutes, Proteobacteria, and Actinomycetes are the dominant phyla in the gut bacterial community of S. frugiperda , which was similar to that of many lepidopteran insects, including Lymantria dispar asiatica , Lymantria xylina , Grapholita molesta , Spodoptera exigua , and so on 2 , 3 , 40 , 41 . The difference in the abundance of these phyla in S. frugiperda gut was also observed, which could be explained by different sampling areas, environments, and sequencing technology 42 , 43 .…”

Section: Discussionsupporting

confidence: 62%

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The bacterial and fungal communities of the larval midgut of Spodoptera frugiperda (Lepidoptera: Noctuidae) varied by feeding on two cruciferous vegetables

Yuning

Luyang

Xueming

et al. 2022

Sci Rep

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Spodoptera frugiperda is a highly polyphagous pest worldwide with a wide host range that causes serious losses to many economically important crops. Recently, insect-microbe associations have become a hot spot in current entomology research, and the midgut microbiome of S. frugiperda has been investigated, while the effects of cruciferous vegetables remain unknown. In this study, the growth of S. frugiperda larvae fed on an artificial diet, Brassica campestris and Brassica oleracea for 7 days was analyzed. Besides, the microbial community and functional prediction analyses of the larval midguts of S. frugiperda fed with different diets were performed by high-throughput sequencing. Our results showed that B. oleracea inhibited the growth of S. frugiperda larvae. The larval midgut microbial community composition and structure were significantly affected by different diets. Linear discriminant analysis effect size (LEfSe) suggested 20 bacterial genera and 2 fungal genera contributed to different gut microbial community structures. The functional classification of the midgut microbiome analyzed by PICRUSt and FUNGuild showed that the most COG function categories of midgut bacterial function were changed by B. oleracea, while the guilds of fungal function were altered by B. campestris significantly. These results showed that the diversity and structure of the S. frugiperda midgut microbial community were affected by cruciferous vegetable feeding. Our study provided a preliminary understanding of the role of midgut microbes in S. frugiperda larvae in response to cruciferous vegetables.

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Section: Discussionsupporting

confidence: 62%

“…Our results further support the conclusion that gut fungal communities could also be affected by the host species 38 , 39 . Therefore, a revelation emerged that regulating the gut microbiota could be a potential approach for the control of S. frugiperda 40 .…”

Section: Discussionmentioning

confidence: 99%

The bacterial and fungal communities of the larval midgut of Spodoptera frugiperda (Lepidoptera: Noctuidae) varied by feeding on two cruciferous vegetables

Yuning

Luyang

Xueming

et al. 2022

Sci Rep

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show abstract

“…Proteobacteria, Actinobacteria, and Firmicutes dominate the Bemisia tabaci larval guts (Santos‐Garcia et al, 2020). Proteobacteria and Firmicutes are enriched in the larvae of C. capitata (Malacrino et al, 2018), Phthorimaea operculella (Zheng et al, 2020), Spodoptera exigua (Gao et al, 2020), Lymantria dispar asiatica (Zeng et al, 2020), and Monochamus alternatus (Guo et al, 2020). Therefore, the predominance of Proteobacteria and Firmicutes in insects is likely the result of long‐term coevolution.…”

Section: Discussionmentioning

confidence: 99%

“…Lymantria dispar asiatica (Zeng et al, 2020), and Monochamus alternatus (Guo et al, 2020). Therefore, the predominance of Proteobacteria and Firmicutes in insects is likely the result of long-term coevolution.…”

Section: Discussionmentioning

confidence: 99%

A switch of microbial flora coupled with ontogenetic niche shift in Leptinotarsa decemlineata

Kang

Jin

et al. 2021

Arch Insect Biochem Physiol

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In Leptinotarsa decemlineata, a final‐instar wandering larva typically undergoes an ontogenetic niche shift (ONS), from potato plant during the foraging stage to its pupation site below ground. Using high‐throughput sequencing of the bacterial 16S ribosomal RNA gene, we determined the hypothesis that the L. decemlineata pupae harbor stage‐specific bacteria to meet the physiological requirements for underground habitat. We identified 34 bacterial phyla, comprising 73 classes, 208 orders, 375 families, and 766 genera in the collected specimens. Microbes across phyla Proteobacteria, Firmicutes, Actinobacteria, and Bacteroidetes were enriched in the pupae, while those in the phylum Proteobacteria, Tenericutes, Firmicutes, and Bacteroidetes dominated in the larvae and adults. A total of 18 genera, including Blastococcus, Corynebacterium_1, Gordonia, Microbacterium, Nocardia, Nocardioides, Rhodococcus, Solirubrobacter, Tsukamurella, Enterococcus, Acinetobacter, Escherichia_Shigella, Lysobacter, Pseudomonas, and Stenotrophomonas, were specifically distributed in pupae. Moreover, soil sterilizing removed a major portion of bacteria in pupae. Specifically, both Enterococcus and Pseudomonas were eliminated in the soil sterilizing and antibiotic‐fed beetle groups. Furthermore, the pupation rate and fresh pupal weight were similar, whereas the emergence rate and adult weight were decreased in the antibiotic‐fed beetles, compared with controls. The results demonstrate that a switch of bacterial communities occurs in the pupae; the pupal‐specific bacteria genera are mainly originated from soil; this bacterial biodiversity improves pupa performance in soil. Our results provide new insight into the evolutionary fitness of L. decemlineata to different environmental niches.

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“…We have previously documented that the gut microbial community in Lymantria dispar asiatic is regulated by environmental temperature (Zeng, Shi, Guo, et al, 2019), dietary pH (Zeng, Shi, Shi, et al, 2019), and avermectin stress (Zeng et al, 2020). Previous studies have suggested that gut microbial communities in insects contribute to various biological processes, including pheromone production, pesticide degradation, vitamin synthesis, and pathogen prevention (Reeson et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Diel pattern in the structure and function of the gut microbial community inLymantria dispar asiatica(Lepidoptera: Lymantriidae) larvae

Zeng

Vuong

Guo

et al. 2020

Arch Insect Biochem Physiol

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In the present study, diel pattern in gut microbial communities in insects were evaluated. Lymantria dispar asiatica fourth instar larvae (72 ± 2 hr after molting) at noon (LdD) and midnight (LdN) were used for a comparative analysis of the gut microbial community. Ten bacterial operational taxonomic units (OTUs) were shared between LdD and LdN samples. One bacterial OTU was specific to LdD. The dominant gut microbes were OTU72 in LdD and OTU75 in LdN. A linear discriminant analysis effect size cladogram suggested that ten bacterial OTUs maintain significant differences in relative abundances between LdD and LdN. These results agreed with the discrete ellipses between LdD and LdN in principal coordinates analysis plots. Additionally, using phylogenetic investigation of communities by reconstruction of unobserved states, the gut microbial community was assigned to 23 functional terms, among which 22 exhibited significant differences between LdD and LdN. To conclude, the present study documented a diel pattern in the gut microbial community of L. dispar asiatica larvae. K E Y W O R D S circadian, gut microbial community, Lymantria dispar asiatica larvae Jian-Yong Zeng and Thi-Minh-Dien Vuong contributed equally to this work.

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Avermectin stress varied structure and function of gut microbial community in Lymantria dispar asiatica (Lepidoptera: Lymantriidae) larvae

Cited by 22 publications

References 35 publications

The bacterial and fungal communities of the larval midgut of Spodoptera frugiperda (Lepidoptera: Noctuidae) varied by feeding on two cruciferous vegetables

The bacterial and fungal communities of the larval midgut of Spodoptera frugiperda (Lepidoptera: Noctuidae) varied by feeding on two cruciferous vegetables

A switch of microbial flora coupled with ontogenetic niche shift in Leptinotarsa decemlineata

Diel pattern in the structure and function of the gut microbial community inLymantria dispar asiatica(Lepidoptera: Lymantriidae) larvae

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