Avian Reoviruses: Propagation, Quantification, and Storage

Abstract

Avian reoviruses (ARVs) are pathogens that cause significant morbidity among commercial poultry. ARVs are prototypic representatives of non‐enveloped viruses that can cause cell‐cell fusion. They belong to the Reoviridae family, which contains many highly pathogenic viruses. ARVs are ubiquitous in commercial poultry and are frequently isolated from the gastrointestinal and respiratory tracts of chickens with acute infections. The virus causes a range of disease states in chicken, including viral arthritis/tenosynovitis, gastroenteritis, hepatitis, myocarditis, “pale bird syndrome,” runting‐stunting syndrome, and respiratory illness. This unit describes avian reovirus propagation, quantification, and storage. Curr. Protoc. Microbiol. 14:15C.2.1‐15C.2.16. © 2009 by John Wiley & Sons, Inc.

Development of broiler chickens after treatment with thymulin 5cH: a zoo technical approach

“…Its structural characteristics permits its survival in the environment, in which its transmission can be horizontal as well as vertical. 36 These viruses are causes of arthritis or synovitis, mainly in leg articulations. Sick birds have difficulty of locomotion, with impact on feed conversion, weight gain and viability.…”

Development of a reverse transcription loop-mediated isothermal amplification assay for visual detection of avian reovirus

“…ARV reference strains and field isolates were propagated in chicken embryo fibroblast cells. Virus titres were determined as described previously (Tran et al, 2009). Avian influenza viruses were grown in 10-day-old specific-pathogen-free chicken embryos.…”

Replication and Oncolytic Activity of an Avian Orthoreovirus in Human Hepatocellular Carcinoma Cells

“…Stocks of virus were generated in CH-SAH cells by successive freeze-thaw cycles and centrifugation. Virus propagation, titration and one-step growth curves were carried out in chicken hepatoma cells (CH-SAH cell line), as described [ 28 ]. To investigate the pre-existing immunity to ARV-PB1, plaque reduction assays were carried out using sera from HCC patients as described [ 29 ].…”

“…Cells in six-well plates were infected with ARV-PB1 at an MOI of 5 for 1 h at room temperature. Subsequently, the inoculum was removed and the cells were washed with phosphate buffered saline (PBS, pH 7.4), and medium was added as described [ 28 ]. Cells were harvested at indicated time points and stored at −80 ° C. Lysates were freeze-thawed three times to release viruses, and the samples were titrated in CH-SAH cells.…”

“…Plaque reduction assays were performed as described [ 28 ] to investigate the presence of neutralizing antibodies in HCC patient sera. Whole blood was collected by venipuncture in a Vacutainer from patients with HCV-induced HCC at the Toronto Western Hospital Liver Clinic (Toronto, ON, Canada).…”