Avidin/PSS membrane microcapsules with biotin-binding activity

Endo, Yuichi; Sato, Katsuhiko; Sugimoto, Katsuyoshi; Anzai, Jun Ichi

doi:10.1016/j.jcis.2011.04.104

Cited by 14 publications

(11 citation statements)

References 33 publications

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“…Some F-BSA may strongly adsorb on the microcapsule membrane. Similar release properties of proteins have been reported on polyelectrolyte microcapsules prepared by using CaCO 3 particles as the template [22][23][24]. F-BSA postloading on the PMMA microcapsule was investigated.…”

Section: Resultssupporting

confidence: 61%

“…CaCO 3 microparticles were prepared as previously reported [22,23]. In brief, 3 mL of 0.33 M CaCl 2 solution was mixed with 1 mL of 1 M Na 2 CO 3 aqueous solution under vigorous agitation (0.1 mg of F-BSA was added to Na 2 CO 3 solution to prepare F-BSAcontaining CaCO 3 microparticles).…”

Section: Preparation Of Pmma Microcapsulesmentioning

confidence: 99%

“…LBL microcapsules have been prepared by coating microparticles with LBL films, followed by the dissolution of the core material. Various functional capsules have been reported by taking advantage of the selective permeability of membrane materials [21][22][23][24][25][26][27][28][29][30][31].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Preparation of poly(methyl methacrylate) microcapsules by in situ polymerization on the surface of calcium carbonate particles

Sato¹,

Nakajima²,

Anzai³

2012

Journal of Colloid and Interface Science

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Section: Resultssupporting

confidence: 61%

Section: Preparation Of Pmma Microcapsulesmentioning

confidence: 99%

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Preparation of poly(methyl methacrylate) microcapsules by in situ polymerization on the surface of calcium carbonate particles

Sato¹,

Nakajima²,

Anzai³

2012

Journal of Colloid and Interface Science

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“…This time was chosen from previous optimization experiments. In the second method 23–24 biotinylated ConA was attached via biotin‐avidin reaction. Sensors surface was initially modified with PEI‐PSS double layer on which avidin (from egg white, Sigma) was deposited by LbL method from solution (1 mg/mL in TRIS‐HCl pH 7.4).…”

Section: Methodsmentioning

confidence: 99%

Sensitivity and Response Time of Polyethyleneimine Modified Impedimetric Transducer for Bacteria Detection

et al. 2015

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An impedimetric transducer based on an interdigitated electrode array (IDEA) modified with polyethyleneimine was used to study interactions with bacteria present in a sample solution. Bacteria immobilization on the sensor surface affects the surface charge and produces changes in the superficial impedance. The sensor surface was chemically modified by layer‐by‐layer (LbL) method with oppositely charged polyelectrolyte layers by alternating polyethyleneimine (PEI) and poly(sodium 4‐styrenesulfonate) (PSS). Escherichia coli gram‐negative bacteria were employed as a model for sensitivity and response time evaluation. Bacteria were immobilized on IDEA with PEI‐PSS‐PEI multilayer taking into account the ability of PEI to react chemically with outer membrane compounds of gram‐negative bacteria and thus improve purely electrostatic interactions. Detection limit of the sensor was found to be as low as 101 cfu/mL in E. coli cultures with response time around 20 minutes. In order to introduce a biorecognition element by bacterial detection, the lectin Concanavalin A (ConA) was deposited on IDEA surface through PEI‐ConA interaction, achieving a detection limit of 104 cfu/mL. The strategies developed show the possibility to achieve highly sensitive and rapid detection of bacteria using IDEA transducers.

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“…Avidin is a glyco-protein isolated from egg white and known to strongly bind biotin or biotin-tagged molecules [6,7]. A variety of biotin-tagged biomolecules have been immobilized on solid surfaces to construct protein architectures and biosensors [8,9,10,11,12,13]. On the other hand, lectin is a family of sugar-binding proteins found in plants and animals [14].…”

Section: Introductionmentioning

confidence: 99%

Recent Progress in Lectin-Based Biosensors

Wang

Anzai

2015

Materials

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This article reviews recent progress in the development of lectin-based biosensors used for the determination of glucose, pathogenic bacteria and toxins, cancer cells, and lectins. Lectin proteins have been widely used for the construction of optical and electrochemical biosensors by exploiting the specific binding affinity to carbohydrates. Among lectin proteins, concanavalin A (Con A) is most frequently used for this purpose as glucose- and mannose-selective lectin. Con A is useful for immobilizing enzymes including glucose oxidase (GOx) and horseradish peroxidase (HRP) on the surface of a solid support to construct glucose and hydrogen peroxide sensors, because these enzymes are covered with intrinsic hydrocarbon chains. Con A-modified electrodes can be used as biosensors sensitive to glucose, cancer cells, and pathogenic bacteria covered with hydrocarbon chains. The target substrates are selectively adsorbed to the surface of Con A-modified electrodes through strong affinity of Con A to hydrocarbon chains. A recent topic in the development of lectin-based biosensors is a successful use of nanomaterials, such as metal nanoparticles and carbon nanotubes, for amplifying output signals of the sensors. In addition, lectin-based biosensors are useful for studying glycan expression on living cells.

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Avidin/PSS membrane microcapsules with biotin-binding activity

Cited by 14 publications

References 33 publications

Preparation of poly(methyl methacrylate) microcapsules by in situ polymerization on the surface of calcium carbonate particles

Preparation of poly(methyl methacrylate) microcapsules by in situ polymerization on the surface of calcium carbonate particles

Sensitivity and Response Time of Polyethyleneimine Modified Impedimetric Transducer for Bacteria Detection

Recent Progress in Lectin-Based Biosensors

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