Avocado Cellulase Extraction and Purification

Awad, Marcel; Lewis, Lowell N.

doi:10.1111/j.1365-2621.1980.tb07577.x

Cited by 34 publications

(16 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Whereas this measure does not account for the tertiary structure of the 695 mature protein, it does reflect their relative acidic or basic nature. The deduced pI for TPP18 of 9.2 is very different from that of avocado protein which is predicted to be 5.3 and has been shown to actually be 4.7 [3]. The apparent basic nature of TPP18 is more similar to that of the bean endo-fl-1,4-glucanase (BAC) which has predicted and measured pI values of 7.8 and 9.5, respectively [30] and of tomCell and tomCel2 which have predicted pls of 7.3 and 8.2, respectively [50].…”

Section: Tppi 8 Encodes a Pistil-predominant Endo-fl-l 4-glucanasementioning

confidence: 78%

Nature and regulation of pistil-expressed genes in tomato

Milligan

Gasser

1995

Plant Mol Biol

View full text Add to dashboard Cite

The specialized reproductive functions of angiosperm pistils are dependent in part upon the regulated activation of numerous genes expressed predominantly in this organ system. To better understand the nature of these pistil-predominant gene products we have analyzed seven cDNA clones isolated from tomato pistils through differential hybridization screening. Six of the seven cDNAs represent sequences previously undescribed in tomato, each having a unique pistil- and/or floral-predominant expression pattern. The putative protein products encoded by six of the cDNAs have been identified by their similarity to sequences in the database of previously sequenced genes, with a seventh sequence having no significant similarity with any previously reported sequence. Three of the putative proteins appear to be targeted to the endomembrane system and include an endo-beta-1,4-glucanase which is expressed exclusively in pistils at early stages of development, and proteins similar in sequence to gamma-thionin and miraculin which are expressed in immature pistils and stamens, and in either sepals or petals, respectively. Two other clones, similar in sequence to each other, were expressed primarily in immature pistils and stamens and encode distinct proteins with similarity to leucine aminopeptidases. An additional clone, which encodes a protein similar in sequence to the enzyme hyoscyamine 6-beta-hydroxylase and to other members of the family of Fe2+/ascorbate-dependent oxidases, was expressed at high levels in pistils, stamens and sepals, and at detectable levels in some vegetative organs. Together, these observations provide new insight into the nature and possible functional roles of genes expressed during reproductive development.

show abstract

Section: Tppi 8 Encodes a Pistil-predominant Endo-fl-l 4-glucanasementioning

confidence: 78%

Nature and regulation of pistil-expressed genes in tomato

Milligan

Gasser

1995

Plant Mol Biol

View full text Add to dashboard Cite

show abstract

“…Cellulose anity chromatography has been used to purify EGs from a range of tissues, including the fruits of avocado (Awad and Lewis 1980), pepper (Ferrarese et al 1995) and apple (Abeles and Biles 1991), as well as bean leaf (Durbin and Lewis 1988), sweet pea anthers (Sexton et al 1990), and elder leaf (Webb et al 1993). A com- Fig.…”

Section: Discussionmentioning

confidence: 99%

Purification and properties of an endo-β-1,4-glucanase from strawberry and down-regulation of the corresponding gene, cel1

Woolley¹,

James

Manning³

2001

Planta

View full text Add to dashboard Cite

An endo-beta-1,4-glucanase (EG) was purified from ripe strawberry (Fragaria x ananassa Duch.) fruit using cellulose affinity chromatography. The purified enzyme gave a single protein band of 54 kDa on sodium dodecyl sulphate-polyacrylamide gel electrophoresis. The N-terminal amino acid sequence of this protein showed strong homology with the proteins encoded by recently identified EG genes from different strawberry cultivars and from Arabidopsis, pepper and tomato. The enzyme specifically cleaved the beta-1,4-glucosyl linkages of xyloglucan but was unable to hydrolyze those of insoluble cellulose. The pH optimum and Km of the enzyme against the artificial substrate carboxymethylcellulose (CMC) were pH 5.0-7.0 and 1.3 mg ml-1, respectively. To assess the role of the Cell enzyme in fruit softening a cDNA of the corresponding fruit-specific and ripening-enhanced strawberry gene, cell, was used to down-regulate cell gene expression in transgenic strawberry plants. In several primary transformants, cell mRNA was strongly suppressed in ripe fruit. However, the EG activity and firmness of these fruit were indistinguishable from those of control fruit. The expression of a second gene, cel2, encoding a different strawberry EG was unaltered in the fruits of these transformants. The presence of the cel2 transcript in transgenic plants may have prevented the specific down-regulation of cell from revealing its role in fruit softening.

show abstract

“…Cellulase was purified from Fuerte avocado fruit as described by Awad and Lewis [1]. Amino acid composition was determined using a Beckman Model 120C amino acid analyzer.…”

Section: Amino Acid Compositionmentioning

confidence: 99%

Avocado cellulase: nucleotide sequence of a putative full-length cDNA clone and evidence for a small gene family

et al. 1987

Self Cite

View full text Add to dashboard Cite

A cDNA library was prepared from ripe avocado fruit (Persea americana Mill. cv. Hass) and screened for clones hybridizing to a 600 bp cDNA clone (pAV5) coding for avocado fruit cellulase. This screening led to the isolation of a clone (pAV363) containing a 2021 nucleotide transcribed sequence and an approximately 150 nucleotide poly(A) tail. Hybridization of pAV363 to a northern blot shows that the length of the homologous message is approximately 2.2 kb. The nucleotide sequence of this putative full-length mRNA clone contains an open reading frame of 1482 nucleotides which codes for a polypeptide of 54.1 kD. The deduced amino acid composition compares favorably with the amino acid composition of native avocado cellulase determined by amino acid analysis. Southern blot analysis of Hind III and Eco RI endonuclease digested genomic DNA indicates a small family of cellulase genes.

show abstract

Avocado Cellulase Extraction and Purification

Cited by 34 publications

References 4 publications

Nature and regulation of pistil-expressed genes in tomato

Nature and regulation of pistil-expressed genes in tomato

Purification and properties of an endo-β-1,4-glucanase from strawberry and down-regulation of the corresponding gene, cel1

Avocado cellulase: nucleotide sequence of a putative full-length cDNA clone and evidence for a small gene family

Contact Info

Product

Resources

About