Axial dispersion in a magnetically stabilized fluidized bed liquid chromatography column

Goetz, V; Graves, Donald H.

doi:10.1016/0032-5910(91)80008-7

Cited by 13 publications

(7 citation statements)

References 19 publications

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“…As an attractive alternative to conventional column chromatography, the liquid MSFB exhibits a combination of the properties of both packed and fluidized bed. These include the efficient fluid−solid mass transfer properties, elimination of solid mixing, low‐pressure drop, resistance to clogging, and continuous countercurrent operation (1). This technique also gives great opportunities for process integration by achieving particulate removal and the capture of product desired in a single operation.…”

Section: Introductionmentioning

confidence: 99%

Application of Magnetic Agarose Support in Liquid Magnetically Stabilized Fluidized Bed for Protein Adsorption

Tong

Sun

2003

Biotechnology Progress

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A novel magnetic agarose support (MAS) was fabricated for application in a liquid magnetically stabilized fluidized bed (MSFB). It was produced by water-in-oil emulsification method using a mixture of agarose solution and nanometer-sized superparamagnetic Fe(3)O(4) particles as the aqueous phase. The MAS showed good superparamagnetic responsiveness in a magnetic field. A reactive triazine dye, Cibacron blue 3GA (CB), was coupled to the gel to prepare a CB-modified magnetic agarose support (CB-MAS) for protein adsorption. Lysozyme was used as a model protein to test the adsorption equilibrium and kinetic behavior of the CB-MAS. The dependence of bed expansion in the MSFB with a transverse magnetic field on liquid velocity and magnetic field intensity was investigated. Liquid-phase dispersion behavior in the MSFB was examined by measurements of residence time distributions and compared with that obtained in packed and expanded beds. Dynamic lysozyme adsorption in the MSFB was also compared with those in packed and expanded beds. The dynamic binding capacity at 10% breakthrough was estimated at 55.8 mg/mL in the MSFB, higher than that in the expanded bed (31.1 mg/mL) at a liquid velocity of 45 cm/h. The results indicate that the CB-MAS is promising for use in liquid MSFB for protein adsorption.

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Section: Introductionmentioning

confidence: 99%

Application of Magnetic Agarose Support in Liquid Magnetically Stabilized Fluidized Bed for Protein Adsorption

Tong

Sun

2003

Biotechnology Progress

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“…Because of the low superficial velocity and bating by magnetic field, the one-dimensional axial dispersion model was used to describe the liquid dispersion in L-S and G-L-S MSB [2,5] . The characteristic of axial liquid dispersion can be described by the axial dispersion coefficient D a .…”

Section: Methodsmentioning

confidence: 99%

“…However, there are few reports about the effects of physical properties of fluids on the axial liquid dispersion coefficients in both L-S and G-L-S MSB. Siegell [1] , Goetz and Graves [2] have experimentally demonstrated the reduction of axial liquid dispersion in the L-S MSB with inert particles by the method of peak detection conductivity measurement, using potassium chloride as the tracer. Goto et al [3] reported that the axial liquid dispersion of the MSB was comparable to that of the packed bed but smaller than that of fluidized bed, using moment analysis of the impulse response curves.…”

Section: Introductionmentioning

confidence: 99%

Axial Liquid Dispersion Characteristics in Magnetically Stabilized Bed

Zhang

Zhang²,

Zhang

et al. 2006

Chinese Journal of Chemical Engineering

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“…In fact, this work repeats the results of Nixon et al (1991) with respect to: field orientation effect, field intensity effect and the number of theoretical plates. Goetz and Graves (1991) used eq. (2.37) to calculate D L .…”

Section: Liquid-solid Beds: Axial Dispersion Coefficients: Experimentmentioning

confidence: 99%

Magnetic Field Assisted Fluidization - A Unified Approach Part 7. Mass Transfer: Chemical reactors, basic studies and practical implementations thereof

Hristov¹

2009

Reviews in Chemical Engineering

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Transfer Studies 5.1. Bubble Columns with chemically inert coarse particles 5.2. Slurry Bubble Columns (SBC) 5.2.7. Slurry Bubble Columns with chemically inert flne particles 5.2.2. Slurry Bubble Columns with fine suspended catalyst particles: laboratory studies 5.2.3. Slurry Bubble Columns with fine suspended catalyst particles: process oriented examples 5.4. Trickle beds (Counter-current flow) 5.4.1. General comments 5.4.2. Flue gas desulphurization -Approach ofGui et al. 5.4.3. Flue gas desulphurization by enhanced iron corrosion in a magnetic field 5.4.4. L-S mass transfer with SRNA-4 catalyst in magnetically assisted trickle bed 5.4.5. Comments and suggestion about the mass transfer dimensionless correlations. 5.4.6. Final comments on magnetically assisted trickle beds 5.5. Magnetically assisted airlifts -mass transfer issues 6. INDUSTRIAL IMPLEMENTATIONS -Trials and errors 6.1. Preamble 6.2. Early attempts: Bulgarian pilot-plant experiments 6.2.7. Results 6.2.2. Principle drawbacks 6.3 The EXXON Era 6.4. The Chinese Wave 6.5. Critical Overview and Lessons 7. CLOSING COMMENTS ACKNOWLEDGMENTS LIST OF SYMBOLS REFERENCES SUMMARY Part 7 of the series Magnetic Field Assisted Fluidization (MFAF)-A Unified Approach is devoted to chemical reactor design utilizing Brought to you by | University of Birmingham Authenticated Download Date | 6/13/15 5:09 PM Jordan HristovReviews in Chemical Engineering magnetically assisted fluidized beds. Basic reactor principles wellimplemented in the chemical engineering science are used to analyze the existing situation of those special types of mass transfer devices. The hydrodynamics of all systems reviewed in the previous parts of the series (G-S, L-S and G-L-S) serves as a foundation assuring proper understanding of their mass transfer performances. Thorough analysis of MFAF mass transfer studies in both laboratory and pilot scales is performed. Special attention is paid to the attempts to do industrial implementations of MFAF based chemical reactors: trial-error steps, pitfalls and recent achievements. Part 7 refers only to reactor designs and chemical reaction performances whilst magnetically assisted bioreactors are at issue in a separate part of the review series. All these issues make Part 7 a comprehensive review thoroughly investigating one undeveloped, but with a great potential and directly applicable to practice, branch of magnetic field assisted fluidization. PREFACEDear readers, Part 7 of the series Magnetic Field Assisted Fluidization (MFAF) finally arrived to the point where contacting devices based on this fluidization technique and their mass transfer performances have to be analyzed. As it was mentioned continuously in the previous reviews, the hydrodynamics and the proper knowledge of the regimes enable to understand and control the mass and heat transfer operations in a correct way. Now, with knowledge accumulated systematically in the preceding 6 issues, we approach mass transfer operations performed by various groups of investigators over 45 years and provoke...

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Axial dispersion in a magnetically stabilized fluidized bed liquid chromatography column

Cited by 13 publications

References 19 publications

Application of Magnetic Agarose Support in Liquid Magnetically Stabilized Fluidized Bed for Protein Adsorption

Application of Magnetic Agarose Support in Liquid Magnetically Stabilized Fluidized Bed for Protein Adsorption

Axial Liquid Dispersion Characteristics in Magnetically Stabilized Bed

Magnetic Field Assisted Fluidization - A Unified Approach Part 7. Mass Transfer: Chemical reactors, basic studies and practical implementations thereof

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