Axillary shoots derived from shoot tips in in vitro mass propagation of Anoectochilus formosanus hayata

Winarto, Budi; Samijan,

doi:10.15159/jas.18.11

Cited by 2 publications

(3 citation statements)

References 14 publications

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“…Previously, Vasudevan and Staden [41] also reported that the absence of axillary shoot proliferation in shoot tip explants of Ansellia africana was due to auxin synthesized at the shoot tip explant, that exerted strong apical dominance. However, when compared to another study, the shoot tip explant was found superior to the nodal explant in axillary shoot regeneration of A. formosanus [16].…”

Section: Discussioncontrasting

confidence: 66%

“…Alternatively, micropropagation, a rapid clonal propagation has been a recommended approach implemented for years in the orchid floriculture industry as well as for conservation [12,13]. The effect of explant types such as shoot tip and the nodal segment on micropropagation of jewel orchids was reported previously in A. elatus [14], A. roxburghii [15], A. formosanus [16] and Ludisia discolor [17]. Furthermore, the incorporation of plant growth regulators (PGRs) in a culture medium at suitable concentration and combination was reported to be beneficial in the shoot multiplication of A. setaceus [18,19] and A. elatus [20].…”

Section: Introductionmentioning

confidence: 98%

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Establishment of In Vitro Regeneration Protocol for Sabah’s Jewel Orchid, Macodes limii J.J. Wood & A.L. Lamb

et al. 2022

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Habitat disturbance and excessive collection of wild orchids from their natural habitat have threatened many orchids species at risk of extinction. In this study, the in vitro regeneration protocol for Macodes limii, a jewel orchid endemic to Sabah was established. The effects of explant source and plant growth regulators (PGRs) including naphthaleneacetic acid, picloram, 2,4-dichlorophenoxyacetic acid, 6-benzylaminopurine, kinetin, and thidiazuron on the in vitro regeneration capacity of M. limii plantlets were examined. Both factors showed a significant interaction in promoting axillary shoot formation. Nodal explants from the third and fourth positions cultured with 1.0 mg/L TDZ, induced 95% of shoot regeneration, with an average of three shoots/explant (1.6–1.8 cm of shoot length) after 90 days of culture. The well-developed plantlets went through an acclimatization phase for 60 days with a 60% of survival rate. An inter simple sequence repeat (ISSR) marker analysis confirmed the genetic stability of the in vitro regenerated plants to the mother plant. The successfully acclimatized plantlets were finally transferred to Poring Orchid Conservation Centre for reintroduction. The established protocol provides the means for large-scale production of this endemic jewel orchid, as well as a basis for further research aimed at the conservation and genetic improvement of this plant.

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Section: Discussioncontrasting

confidence: 66%

Section: Introductionmentioning

confidence: 98%

Establishment of In Vitro Regeneration Protocol for Sabah’s Jewel Orchid, Macodes limii J.J. Wood & A.L. Lamb

et al. 2022

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“…Jewel orchid is a common name for several genera in the Orchidaceae family, namely Anoectochilus, Dossinia, Goodyera, Ludisia, and Macodes (De and Pathak 2018). This plant is used for several health care purposes, such as anti-fatigue, anti-oxidant, anti-hyperliposis, anti-tumor, and immune modulation agents (Winarto and Samijan 2018). Due to over-exploitation, this plant group was included in the Vietnam Red Book of endangered species and banned from exploitation for commercial purposes (Vietnam Academy of Science Technology 2007).…”

Section: Introductionmentioning

confidence: 99%

Investigation of the genetic diversity of jewel orchid in Vietnam using RAPD and ISSR markers

Tran

PHAM²,

TRINH³

et al. 2022

Biodiversitas

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Abstract. Tran TKP, Pham MH, Trinh TH, Widiarsih S, Ho VT. 2022. Investigation of the genetic diversity of jewel orchid in Vietnam using RAPD and ISSR markers. Biodiversitas 23: 4816-4825. Jewel orchid is a general name for several genera belonging to the Orchidaceae family, namely Anoectochilus, Doddinia, Goodyera, Ludisia, and Macodes. These plants are generally used as medicinal herbal for common diseases in Vietnam. Due to over-exploitation, the natural resources of jewel orchids are gradually depleted. Therefore, genetic resource research for proper development of this medicinal plant is necessary. This study aimed to investigate the genetic diversity of jewel orchids in Vietnam using RAPD and ISSR markers to provide basic data conservation of genetic resources and the development of this medicinal plant. A total of 20 jewel orchid samples were genetically characterized by using 10 RAPD and 10 ISSR primers. The amplified bands were binary coded and used to construct phylogenetic trees with NTSYSpc 2.1 software. Each RAPD primer produced 5-11 bands with an average of 8.0, and similarity coefficients ranged from 0.52 to 0.81. Each ISSR primer produced 5-10 bands with an average of 7.6, and the similarity coefficients ranged from 0.54 to 0.87. Combined RAPD and ISSR markers produced similarity coefficients ranging from 0.53 to 0.83. Based on the PIC values, the primers used by both markers are highly informative. However, the topology of cluster analysis of 20 jewel orchid accessions does not correspond to the taxa studied. The findings could be potential to employ in future classification, conservation, and development of this plant.

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Axillary shoots derived from shoot tips in in vitro mass propagation of Anoectochilus formosanus hayata

Cited by 2 publications

References 14 publications

Establishment of In Vitro Regeneration Protocol for Sabah’s Jewel Orchid, Macodes limii J.J. Wood & A.L. Lamb

Establishment of In Vitro Regeneration Protocol for Sabah’s Jewel Orchid, Macodes limii J.J. Wood & A.L. Lamb

Investigation of the genetic diversity of jewel orchid in Vietnam using RAPD and ISSR markers

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