“…For lysates, equivalent protein mass was used; for RIP analyses, protein yields were normalized prior to RIP, and then equivalent fractions of the RIPs were analyzed. After transfer, membranes were blocked and probed with primary and HRP-conjugated secondary antibodies as described (36). The following primary antibodies were used: rabbit anti-hnRNP H1 (Abcam, 1:5,000), mouse anti-hnRNP F (Thermo, 1:1,000), mouse anti-hnRNP H1/F (Abcam, 1:1,000), mouse anti-hnRNP K (Abcam, 1:2,000), rabbit anti-Elavl1/HuR (Cell Signaling, 1:1,000), rabbit anti-Nucleolin (Abcam, 1:1,000), indicated.…”