Axotomy increases plasma membrane Ca2+ pump isoform4 in primary afferent neurons

Ogura, Hiroyuki; Tachibana, Tetsuya; Yamanaka, Hiroki; Kobayashi, Kimiko; Obata, Koichi; Dai, Yi; Yoshiya, Shinichi; Noguchi, Koichi

doi:10.1097/wnr.0b013e328011e6e3

Cited by 7 publications

(4 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…One major mechanism for maintaining neuronal calcium homeostasis is calcium‐ATPase (Stys et al, ; Carafoli, ). Ca 2+ ‐ATPase mRNA has been shown to increase after spinal cord injury (Ogura et al, ). Our results show that after peripheral nerve injury, there is a statistically significant upregulation of Ca 2+ ‐ATPase mRNA.…”

Section: Discussionmentioning

confidence: 99%

Best time window for the use of calcium‐modulating agents to improve functional recovery in injured peripheral nerves—An experiment in rats

Yan

Shen

Agresti

et al. 2017

J of Neuroscience Research

View full text Add to dashboard Cite

Peripheral nerve injury can have a devastating effect on daily life. Calcium concentrations in nerve fibers drastically increase after nerve injury, and this activates downstream processes leading to neuron death. Our previous studies showed that calcium-modulating agents decrease calcium accumulation, which aids in regeneration of injured peripheral nerves; however, the optimal therapeutic window for this application has not yet been identified. In this study, we show that calcium clearance after nerve injury is positively correlated with functional recovery in rats suffering from a crushed sciatic nerve injury. After the nerve injury, calcium accumulation increased. Peak volume is from 2 to 8 weeks post injury; calcium accumulation then gradually decreased over the following 24-week period. The compound muscle action potential (CMAP) measurement from the extensor digitorum longus muscle recovered to nearly normal levels in 24 weeks. Simultaneously, real-time polymerase chain reaction results showed that upregulation of calcium-ATPase (a membrane protein that transports calcium out of nerve fibers) mRNA peaked at 12 weeks. These results suggest that without intervention, the peak in calcium-ATPase mRNA expression in the injured nerve occurs after the peak in calcium accumulation, and CMAP recovery continues beyond 24 weeks. Immediately using calcium-modulating agents after crushed nerve injury improved functional recovery. These studies suggest that a crucial time frame in which to initiate effective clinical approaches to accelerate calcium clearance and nerve regeneration would be prior to 2 weeks post injury. © 2017 Wiley Periodicals, Inc.

show abstract

Section: Discussionmentioning

confidence: 99%

Best time window for the use of calcium‐modulating agents to improve functional recovery in injured peripheral nerves—An experiment in rats

Yan

Shen

Agresti

et al. 2017

J of Neuroscience Research

View full text Add to dashboard Cite

show abstract

“…AT promotes the activity of the plasmatic membrane Ca 2+ ATPase (PMCA), which regulates intracellular Ca 2+ concentration by taking calcium out from the cell [24]. In addition, AT promotes the expression of PMCA in dorsal root ganglia [25].…”

Section: Calcium Dynamics In Neurons and Glia After Axotomymentioning

confidence: 99%

Calcium in Neuronal and Glial Response to Axotomy

Khaitin

2021

IJMS

View full text Add to dashboard Cite

Neurotrauma assumes an instant or delayed disconnection of axons (axotomy), which affects not only neurons, but surrounding glia as well. Not only mechanically injured glia near the site of disconnection, especially transection, is subjected to the damage, but also glia that is remote from the lesion site. Glial cells, which surround the neuronal body, in turn, support neuron survival, so there is a mutual protection between neuron and glia. Calcium signaling is a central mediator of all post-axotomy events, both in neuron and glia, playing a critical role in their survival/regeneration or death/degeneration. The involvement of calcium in post-axotomy survival of the remote, mechanically intact glia is poorly studied. The purpose of this review is to sum up the calcium-involving mechanisms in responses of neurons and glial cells to axotomy to show their importance and to give some suggestions for future research of remote glia in this context.

show abstract

“…PMCA2 gene expression is down regulated by more the 50% following contusive spinal cord injury [100]. Axotomy decreases expression of PMCA isoforms 1–3 in large DRG neurons but increases isoform 4 in small sensory neurons [74]. DRG neurons of different size convey information from different sensory modalities, suggesting that changes in PMCA expression may contribute to injury–induced changes in sensory perception.…”

Section: The Role Of Pmcas In Altered Sensory Functionmentioning

confidence: 99%

Evidence for a role of plasma membrane calcium pumps in neurodegenerative disease: Recent developments

Strehler

Thayer

2018

Neuroscience Letters

View full text Add to dashboard Cite

Plasma membrane Ca ATPases (PMCAs) are a major system for calcium extrusion from all cells. Different PMCA isoforms and splice variants are involved in the precise temporal and spatial handling of Ca signals and the re-establishment of resting Ca levels in the nervous system. Lack or inappropriate expression of specific PMCAs leads to characteristic neuronal phenotypes, which may be reciprocally exacerbated by genetic predisposition through alleles in other genes that modify PMCA interactions, regulation, and function. PMCA dysfunction is often poorly compensated in neurons and may lead to changes in synaptic transmission, altered excitability and, with long-term calcium overload, eventual cell death. Decrease and functional decline of PMCAs are hallmarks of neurodegeneration during aging, and mutations in specific PMCAs are responsible for neuronal dysfunction and accelerated neurodegeneration in many sensory and cognitive diseases.

show abstract

Axotomy increases plasma membrane Ca2+ pump isoform4 in primary afferent neurons

Cited by 7 publications

References 23 publications

Best time window for the use of calcium‐modulating agents to improve functional recovery in injured peripheral nerves—An experiment in rats

Best time window for the use of calcium‐modulating agents to improve functional recovery in injured peripheral nerves—An experiment in rats

Calcium in Neuronal and Glial Response to Axotomy

Evidence for a role of plasma membrane calcium pumps in neurodegenerative disease: Recent developments

Contact Info

Product

Resources

About