Azithromycin as a new chiral selector in capillary electrophoresis

Kumar, Avvaru Praveen; Park, Jung Hag

doi:10.1016/j.chroma.2010.12.106

Cited by 47 publications

(35 citation statements)

References 32 publications

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“…Enantioseparation can be achieved using chiral selectors, which discriminate between the enantiomers by an enantioselective complexation between the enantiomers of the analyte and the chiral selector giving rise to differences in the electrophoretic mobility of the enantiomers [13,21,[23][24][25]. Different kinds of chiral selectors are currently available, such as cyclodextrins (CDs) and its derivatives [26][27][28][29][30][31], chiral crown ethers [32], proteins [33], chiral surfactants, macrocyclic antibiotics [34,35], ligand-exchange complexes [36] and polysaccharides [37]. Additionally, the CE technique allows for the easy determination of physico-chemical constants (e.g.…”

Section: Introductionmentioning

confidence: 99%

Capillary electrophoresis method for the determination of isradipine enantiomers: Stability studies and pharmaceutical formulation analysis

2011

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A simple enantioselective method based on CE using CD as chiral selector was developed and validated for the determination of isradipine (IRD) enantiomers in a pharmaceutical formulation and for the determination of IRD enantiomers in degradation studies. After optimization, the best results were obtained using 15 mM borate buffer at pH 9.3 and sulfobutyl ether-β-cyclodextrin (2.5%, w/v) as chiral selector. The applied voltage was +30 kV, and the sample injection was performed in the hydrodynamic mode. All analyses were carried out in a fused-silica uncoated capillary with an id of 50 μm and total length of 60.0 cm. Under these conditions, a complete separation between IRD enantiomers was achieved in less than 7 min. Linearity was obtained in the range 50-300 μg/mL for both enantiomers (r≥0.9978). The RSD (%) and relative errors (%) obtained in precision and accuracy studies (intra-day and inter-day) were lower than 5%. Therefore, this method was found to be appropriate for controlling pharmaceutical formulations containing IRD enantiomers and the assay was considered to be stability indicating. The drug was subjected to oxidation, hydrolysis and photolysis. In all stress conditions the drug presented considerable degradation when compared with a fresh sample (zero time).

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Section: Introductionmentioning

confidence: 99%

Capillary electrophoresis method for the determination of isradipine enantiomers: Stability studies and pharmaceutical formulation analysis

2011

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“…The advantages of CE, compared with other chromatographic methods, are that it is a simple, rapid, and practical method for providing high separation efficiency that requires small amounts of samples and reagents. Different types of chiral selectors, including cyclodextrins (CDs) and their derivatives [8][9][10], polysaccharides [11,12], antibiotics [13][14][15][16][17][18], and metal ion complexes [19][20][21][22][23], have been successfully applied as chiral additives in CE for chiral separations.…”

Section: Introductionmentioning

confidence: 99%

Investigation of Enantiomeric Separation of Chiral Drugs by CE Using Cu(II)–Clindamycin Complex as a Novel Chiral Selector

Liu

Wang

et al. 2011

Chromatographia

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The enantiomeric separation of several basic drugs was investigated using copper(II)-clindamycin as a new chiral selector. The results show that the chiral selector allows high-resolution separation of some racemic basic drugs, including tropicamide, propranolol, sotalol, bisoprolol, epinephrine, esmolol, atenolol, and metoprolol. The enantioselectivity was influenced by parameters such as the type of metal ion, ratio of clindamycin and Cu(II), pH of the background electrolyte, clindamycin concentration, applied voltage, and capillary temperature. The optimal separation conditions were determined to be 20 mM clindamycin/10 mM Cu 2? , pH 9.06, at 20 kV and 22°C within 25 min.

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“…Other reported methods are mostly based on chromatographic techniques. These include capillary zone electrophoresis [10] and high performance liquid chromatography (HPLC) [11][12][13][14][15][16][17] using UV detector [11][12][13][14], diode-array detector [15], electrochemical detector [16], fluorescence detector after precolumn derivatization [17,18] and liquid chromatography-mass spectrometry or LC-MS/MS [19][20][21][22][23].…”

Section: Introductionmentioning

confidence: 99%

LC Determination and Stability Assessment of Macrolide Antibiotics Azithromycin and Spiramycin in Bulk and Tablet Samples

Mahmoudi

2015

ILNS

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ABSTRACT. Development and validation of rapid HPLC method for quantifying macrolide antibiotics azithromycin (AZI) and spiramycin (SPI) in bulk and tablet samples is described. Determination was performed on a reversed phase C 18 ODB column (250×4.6 nm I.D) at ambient temperature, and employing a UV-detection set at 210 nm. The mobile phase consists of acetonitrile -2-methyl-2-propanol-hydrogenphosphate buffer, pH 6.2, with 1.8% triethylamine (32:8: up to 100, v/v/v), delivered at a flow-rate of 1.1 mL min -1 . The assay is linear in concentration ranges of: 0.004-4.8 and 0.0003-1.2 mg mL −1 for azithromycin and spiramycin, respectively, with detection limit of 0.02% for SPI and 0.03% for AZI. Recovery experiments revealed recovery of 98.51-100.82%. The applicability of this method in stability assessment studies is evaluated.

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Azithromycin as a new chiral selector in capillary electrophoresis

Cited by 47 publications

References 32 publications

Capillary electrophoresis method for the determination of isradipine enantiomers: Stability studies and pharmaceutical formulation analysis

Capillary electrophoresis method for the determination of isradipine enantiomers: Stability studies and pharmaceutical formulation analysis

Investigation of Enantiomeric Separation of Chiral Drugs by CE Using Cu(II)–Clindamycin Complex as a Novel Chiral Selector

LC Determination and Stability Assessment of Macrolide Antibiotics Azithromycin and Spiramycin in Bulk and Tablet Samples

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