Azithromycin Inhibits Mucus Hypersecretion from Airway Epithelial Cells

Shimizu, Takeshi; Shimizu, Shino

doi:10.1155/2012/265714

Cited by 31 publications

(27 citation statements)

References 29 publications

(37 reference statements)

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“…Our results showed that AZM inhibited total mucin, and MUC5AC gene and protein expression in NCI-H292 cells. This is in line with previous reports [4,17,18]. …”

Section: Discussionsupporting

confidence: 94%

“…One previous study reported that josamycin (a 16-membered macrolide) did not inhibit MUC5AC production [4]. However, in the present study, we found that MDM (a 16-membered macrolide) inhibited mucin secretion from airway epithelial cells similarly to AZM (a 15-membered macrolide).…”

Section: Discussioncontrasting

confidence: 83%

“…Several in vitro studies have demonstrated that 14- and 15-membered macrolides inhibit MUC5AC induction by inflammatory stimuli [4,15,16]. Our results showed that AZM inhibited total mucin, and MUC5AC gene and protein expression in NCI-H292 cells.…”

Section: Discussionsupporting

confidence: 64%

“…Roxithromycin (14-membered) and azithromycin (AZM; 15-membered) inhibit MUC5AC secretion from human airway epithelial cells (NCI-H292) [3,4]. Tumor necrosis factor-α (TNF-α) is a well-known stimulant for secretion and gene expression of airway mucin.…”

Section: Introductionmentioning

confidence: 99%

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Macrolides Attenuate Phorbol Ester-Induced Tumor Necrosis Factor-α and Mucin Production from Human Airway Epithelial Cells

Poachanukoon¹,

Koontongkaew

Monthanapisut

et al. 2014

Pharmacology

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Background/Aims: Macrolide antibiotics are effective drugs in chronic bronchiolitis and chronic rhinosinusitis with mucus hypersecretion. However, the mechanism of action is unclear. This study was designed to investigate the effect of azithromycin (AZM; 15-membered) and midecamycin acetate (MDM; 16-membered) on MUC5AC and MUC2 gene expression and secretion from human airway epithelial cells. The effects of the two macrolides on tumor necrosis factor-α (TNF-α) release were also examined. Methods: Confluent NCI-H292 human mucoepidermoid airways epithelial cells were pretreated with AZM or MDM for 2 h and then stimulated with 200 nmol/l phorbol 12-myristate 13-acetate (PMA) for 8 h. The MUC5AC and MUC2 gene expression was measured by real-time quantitative RT-PCR. Total mucin in culture supernatants was measured using enzyme-linked lectin assay. Enzyme-linked immunosorbent assay was used to determine MUC5AC, MUC2 and TNF-α released by the cells. Results: AZM and MDM attenuated PMA-induced MUC5AC and MUC2 gene and protein expression in NCI-H292 cells. They also suppressed PMA-mediated TNF-α in the cells. Conclusion: The present study demonstrates that AZM and MDM suppress the synthesis of mucin and TNF-α from human airway epithelial cells.

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“…Our results showed that AZM inhibited total mucin, and MUC5AC gene and protein expression in NCI-H292 cells. This is in line with previous reports [4,17,18]. …”

Section: Discussionsupporting

confidence: 94%

Section: Discussioncontrasting

confidence: 83%

Section: Discussionsupporting

confidence: 64%

Section: Introductionmentioning

confidence: 99%

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Macrolides Attenuate Phorbol Ester-Induced Tumor Necrosis Factor-α and Mucin Production from Human Airway Epithelial Cells

Poachanukoon¹,

Koontongkaew

Monthanapisut

et al. 2014

Pharmacology

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“…MUC5AC mRNA expression was also significantly lower in macrolide-treated NCI-H292 cells and human nasal epithelial cells than controls [76]. Significantly, these results for CAM and AZM have been reproduced in a separate study [77]. The reduced MUC5AC mRNA transcription was suggested to be via macrolide inhibition of IL-13 signalling pathways [78].…”

Section: Inhibition Of Mucin Secretionmentioning

confidence: 99%

Novel Therapies to Inhibit Mucus Synthesis and Secretion in Airway Hypersecretory Diseases

Rogers²

2015

Pharmacology

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Background: In asthma and chronic obstructive pulmonary disease (COPD), airway mucus hypersecretion contributes to impaired mucociliary clearance, mucostasis and, potentially, the development of mucus plugging of the airways. Summary: Excess mucus production can be targeted via therapies that focus on inhibition mucin synthesis, via reducing expression of mucin (MUC) genes, and/or inhibition of mucin secretion into the airways. Key Messages: This review discusses a number of therapeutic approaches to reduce airway mucus in asthma and COPD, including the use of synthetic and natural products. In particular, it highlights areas where clinical trials of inhibitors of particular target molecules are lacking. Epidermal growth factor receptor (EGFR) tyrosine kinase inhibitors are an example of a targeted therapy that has been researched to reduce mucus synthesis, as have inhibitors of EGFR's downstream signalling pathways, for example, mitogen-activated protein kinase-13 and hypoxia inducible factor-1. However, their efficacy and safety profiles are currently not up to the mark. There is clinical potential in Bio-11006, which reduces mucus secretion via the inhibition of myristoylated alanine-rich C-kinase substrate and is currently in Phase IIb trial.

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Compressed sensing approach for wrist vein biometrics

Lantsov

Ryabko

Shchekin

2017

Journal of Biophotonics

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The work describes features of the compressed sensing (CS) approach utilized for development of a wearable system for wrist vein recognition with single-pixel detection; we consider this system useful for biometrics authentication purposes. The CS approach implies use of a spatial light modulation (SLM) which, in our case, can be performed differently-with a liquid crystal display or diffusely scattering medium. We show that compressed sensing combined with above-mentioned means of SLM allows us to avoid using an optical system-a limiting factor for wearable devices. The trade-off between the 2 different SLM approaches regarding issues of practical implementation of CS approach for wrist vein recognition purposes is discussed. A possible solution of a misalignment problem-a typical issue for imaging systems based upon 2D arrays of photodiodes-is also proposed. Proposed design of the wearable device for wrist vein recognition is based upon single-pixel detection.

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Azithromycin Inhibits Mucus Hypersecretion from Airway Epithelial Cells

Cited by 31 publications

References 29 publications

Macrolides Attenuate Phorbol Ester-Induced Tumor Necrosis Factor-α and Mucin Production from Human Airway Epithelial Cells

Macrolides Attenuate Phorbol Ester-Induced Tumor Necrosis Factor-α and Mucin Production from Human Airway Epithelial Cells

Novel Therapies to Inhibit Mucus Synthesis and Secretion in Airway Hypersecretory Diseases

Compressed sensing approach for wrist vein biometrics

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