B group vitamins and cleft lip and cleft palate

Schubert, Johannes; Schmidt, Reiner; Syska, E.

doi:10.1054/ijom.2001.0212

Cited by 26 publications

(20 citation statements)

References 19 publications

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“…Complete CP only occurred in embryos of dams fed the FAD diet for 8 weeks, suggesting that a substantial reduction in tissue folate is able to induce CP. This may explain why Schubert et al [2002] and Burgoon et al [2002], who administered FAD diets to female mice for 2 and 4 weeks, respectively, did not find the occurrence of CP in their progeny. Interestingly, although complete CP was not observed in the embryos of dams fed the FAD diet for 2-6 weeks, alterations in many of the mechanisms causing palatal-shelf fusion were observed in vitro in palates of embryos of dams fed the FAD diet for 2 weeks (see below).…”

Section: Discussionmentioning

confidence: 99%

“…However, studies carried out on mice are not conclusive on whether or not FA deficiency leads to CP [Burgoon et al, 2002;Schubert et al, 2002]. Only a delay in palate development has been observed in the progeny of female mice which had been on an FA-deficient (FAD) diet for 4 weeks [Burgoon et al, 2002], and the mechanisms by which FA acts during palate development remain unknown.…”

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confidence: 99%

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Occurrence of Cleft-Palate and Alteration of Tgf-β3 Expression and the Mechanisms Leading to Palatal Fusion in Mice following Dietary Folic-Acid Deficiency

Maldonado

Murillo

Barrio

et al. 2011

Cells Tissues Organs

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Folic acid (FA) is essential for numerous bodily functions. Its decrease during pregnancy has been associated with an increased risk of congenital malformations in the progeny. The relationship between FA deficiency and the appearance of cleft palate (CP) is controversial, and little information exists on a possible effect of FA on palate development. We investigated the effect of a 2–8 weeks’ induced FA deficiency in female mice on the development of CP in their progeny as well as the mechanisms leading to palatal fusion, i.e. cell proliferation, cell death, and palatal-shelf adhesion and fusion. We showed that an 8 weeks’ maternal FA deficiency caused complete CP in the fetuses although a 2 weeks’ maternal FA deficiency was enough to alter all the mechanisms analyzed. Since transforming growth factor-β₃ (TGF-β₃) is crucial for palatal fusion and since most of the mechanisms impaired by FA deficiency were also observed in the palates of Tgf-β₃null mutant mice, we investigated the presence of TGF-β₃ mRNA, its protein and phospho-SMAD2 in FA-deficient (FAD) mouse palates. Our results evidenced a large reduction in Tgf-β₃ expression in palates of embryos of dams fed an FAD diet for 8 weeks; Tgf-β₃ expression was less reduced in palates of embryos of dams fed an FAD diet for 2 weeks. Addition of TGF-β₃ to palatal-shelf cultures of embryos of dams fed an FAD diet for 2 weeks normalized all the altered mechanisms. Thus, an insufficient folate status may be a risk factor for the development of CP in mice, and exogenous TGF-β₃ compensates this deficit in vitro.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Occurrence of Cleft-Palate and Alteration of Tgf-β3 Expression and the Mechanisms Leading to Palatal Fusion in Mice following Dietary Folic-Acid Deficiency

Maldonado

Murillo

Barrio

et al. 2011

Cells Tissues Organs

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“…The importance of B vitamins (besides folates) is supported by both animal studies [50,51], and studies in humans [25][26][27]. Wong et al [52] found a significantly decreased B6 level together with an increased homocysteine level in 35 case mothers compared to 56 control mothers and suggested that a protective effect of vitamin B6 might act through a lowering of hyperhomocysteinemia in the mother.…”

Section: Vitaminsmentioning

confidence: 99%

Oral clefts and life style factors — A case-cohort study based on prospective Danish data

et al. 2007

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This study examines the association between oral clefts and first trimester maternal lifestyle factors based on prospective data from the Danish National Birth Cohort. The cohort includes approximately 100,000 pregnancies. In total 192 mothers gave birth to child with an oral cleft during 1997-2003. Information on risk factors such as smoking, alcohol consumption, tea, coffee, cola, and food supplements was obtained during pregnancy for these and 828 randomly selected controls. We found that first trimester maternal smoking was associated with an increased risk of oral clefts (odds ratio (OR): 1.50; 95% confidence interval (CIs): 1.05, 2.14). Although not statistically significant, we also saw associations with first trimester consumption of alcohol (OR: 1.11; CIs: 0.79, 1.55), tea (OR: 1.31; CIs: 0.93, 1.86), and drinking more than 1 l of cola per week (OR: 1.40; CIs: 0.92, 2.12). Furthermore supplementation with > or =400 mcg folic acid daily during the entire first trimester (OR: 0.75; CIs: 0.46, 1.22) suggested an inverse associated with oral clefts, similar to our results on coffee drinking. No effects were found for smaller doses of folic acid, vitamin A, B6 or B12 in this study. The present study found an association between oral clefts and smoking and, although not conclusive, supports an association of oral cleft with alcohol.

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“…The A/WySnJ strain, an inbred strain of F268 generation has minimal genetic variance (www.jax.org) and develops cleft lip with or without cleft palate at a high spontaneous rate 13,14,19 of 38-50% 20,22 . The NMRI mice, an out-bred strain, were used to compare the variability of spontaneous CL/P.…”

Section: Methodsmentioning

confidence: 99%

In vitro investigation of the secondary palate development in two strains of mice

Scheller

Schubert²,

Schubert

2011

International Journal of Oral and Maxillofacial Surgery

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B group vitamins and cleft lip and cleft palate

Cited by 26 publications

References 19 publications

Occurrence of Cleft-Palate and Alteration of <i>Tgf-</i>β<sub><i>3</i></sub> Expression and the Mechanisms Leading to Palatal Fusion in Mice following Dietary Folic-Acid Deficiency

Occurrence of Cleft-Palate and Alteration of <i>Tgf-</i>β<sub><i>3</i></sub> Expression and the Mechanisms Leading to Palatal Fusion in Mice following Dietary Folic-Acid Deficiency

Oral clefts and life style factors — A case-cohort study based on prospective Danish data

In vitro investigation of the secondary palate development in two strains of mice

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