B-type response regulator hst1 controls salinity tolerance in rice by regulating transcription factors and antioxidant mechanisms

Aycan, Murat; Nahar, L; Baslam, Marouane; Mitsui, Toshiaki

doi:10.1016/j.plaphy.2023.02.008

Cited by 16 publications

(16 citation statements)

References 89 publications

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“…Especially under multiple HT+DS conditions, the YNU genotype promotes the A n in the YNU genotype. A similar result was found by Aycan et al [35] that the YNU genotype with the hst1 gene supports the photosynthesis rate also under salt stress conditions.…”

Section: Discussionsupporting

confidence: 89%

“…The lower production of ROS by the effect of the hst1 gene resulted in less MDA production. Previously, under salinity and multiple salinity and high−temperature stress, the YNU genotype with the hst1 gene showed a similar pattern [35,49].…”

Section: Discussionmentioning

confidence: 80%

“…It possesses a C−terminal Myb−like DNA−binding domain and an N−terminal receiver domain, which confer specificity to the subsequent responses [31,32]. Recent research has suggested that the A. thaliana ARR1, ARR10, and ARR12 genes, orthologous to OsRR22 in rice, function as negative regulators of tolerance to drought [33] and salinity [34,35]. Although the hst1 gene provides salinity tolerance in rice [35,36], Nahar et al [37] highlighted that the YNU31−2−4 rice genotype carrying the hst1 gene provided higher photosynthetic activity, lower Na + ion accumulation, and high yield potential under multiple heat and salt stress.…”

Section: Introductionmentioning

confidence: 99%

“…Recent research has suggested that the A. thaliana ARR1, ARR10, and ARR12 genes, orthologous to OsRR22 in rice, function as negative regulators of tolerance to drought [33] and salinity [34,35]. Although the hst1 gene provides salinity tolerance in rice [35,36], Nahar et al [37] highlighted that the YNU31−2−4 rice genotype carrying the hst1 gene provided higher photosynthetic activity, lower Na + ion accumulation, and high yield potential under multiple heat and salt stress. It was previously known that the ARR1, ARR10, and ARR12 genes in Arabidopsis support the plants' survival under drought stress [33].…”

Section: Introductionmentioning

confidence: 99%

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The Promising B−Type Response Regulator hst1 Gene Provides Multiple High Temperature and Drought Stress Tolerance in Rice

Lopes Hornai,

Aycan,

Mitsui

2024

IJMS

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High temperatures, drought, and salt stresses severely inhibit plant growth and production due to the effects of climate change. The Arabidopsis ARR1, ARR10, and ARR12 genes were identified as negative salt and drought stress regulators. However, in rice, the tolerance capacity of the hst1 gene, which is orthologous to the ARR1, ARR10, and ARR12 genes, to drought and multiple high temperature and drought stresses remains unknown. At the seedling and reproductive stages, we investigated the drought (DS) high temperature (HT) and multiple high temperature and drought stress (HT+DS) tolerance capacity of the YNU31−2−4 (YNU) genotype, which carries the hst1 gene, and its nearest genomic relative Sister Line (SL), which has a 99% identical genome without the hst1 gene. At the seedling stage, YNU demonstrated greater growth, photosynthesis, antioxidant enzyme activity, and decreased ROS accumulation under multiple HT+DS conditions. The YNU genotype also demonstrated improved yield potential and grain quality due to higher antioxidant enzyme activity and lower ROS generation throughout the reproductive stage under multiple HT+DS settings. Furthermore, for the first time, we discovered that the B−type response regulator hst1 gene controls ROS generation and antioxidant enzyme activities by regulating upstream and downstream genes to overcome yield reduction under multiple high temperatures and drought stress. This insight will help us to better understand the mechanisms of high temperature and drought stress tolerance in rice, as well as the evolution of tolerant crops that can survive increased salinity to provide food security during climate change.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 80%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The Promising B−Type Response Regulator hst1 Gene Provides Multiple High Temperature and Drought Stress Tolerance in Rice

Lopes Hornai,

Aycan,

Mitsui

2024

IJMS

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“…The B-type response regulator hst1 (hitomebore salt-tolerant 1) controls salinity tolerance in rice by regulating transcription factors and antioxidant mechanisms [17]. The introduction of the hst1 gene into rice, coupled with the application of single-nucleotide polymorphism (SNP) marker-assisted selection, led to the development of the BC3FC population YNU31-24, which exhibited a genomic similarity of 93.5% to the parental varieties.…”

Section: Introductionmentioning

confidence: 99%

RNAi-Mediated Suppression of OsBBTI5 Promotes Salt Stress Tolerance in Rice

Lin,

Yi,

Ali

et al. 2024

IJMS

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This study explores the impact of RNAi in terms of selectively inhibiting the expression of the OsBBTI5 gene, with the primary objective of uncovering its involvement in the molecular mechanisms associated with salt tolerance in rice. OsBBTI5, belonging to the Bowman–Birk inhibitor (BBI) family gene, is known for its involvement in plant stress responses. The gene was successfully cloned from rice, exhibiting transcriptional self-activation in yeast. A yeast two-hybrid assay confirmed its specific binding to OsAPX2 (an ascorbate peroxidase gene). Transgenic OsBBTI5-RNAi plants displayed insensitivity to varying concentrations of 24-epibrassinolide in the brassinosteroid sensitivity assay. However, they showed reduced root and plant height at high concentrations (10 and 100 µM) of GA3 immersion. Enzyme activity assays revealed increased peroxidase (POD) and superoxide dismutase (SOD) activities and decreased malondialdehyde (MDA) content under 40-60 mM NaCl. Transcriptomic analysis indicated a significant upregulation of photosynthesis-related genes in transgenic plants under salt stress compared to the wild type. Notably, this study provides novel insights, suggesting that the BBI gene is part of the BR signaling pathway, and that OsBBTI5 potentially enhances stress tolerance in transgenic plants through interaction with the salt stress-related gene OsAPX2.

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