Ba2+ uptake and the inhibition by Ba2+ of K+ flux into rat liver mitochondria

Abstract

Rapid uptake of Ba2+ by respiring rat liver mitochondria is accompanied by a transient stimulation of respiration. Following accumulation of Ba2+, e.g. at a concentration of 120 nmol per mg protein, the mitochondria exhibit reduced rates of state 3 and uncoupler-stimulated respiration. ADP-stimulated respiration is inhibited at a lower concentration of Ba2+ than is required to affect uncoupler-stimulated respiration, suggesting a distinct effect of Ba2+ on mechanisms involved in synthesis of ATP. Ba2+, which has an ionic radius similar to that of K+, inhibits unidirectional K+ flux into respiring rat liver mitochondria. This effect on K+ influx is observable at concentrations of Ba2+, e.g. 23 to 37 nmol per mg protein, which cause no significant change in state 4 or uncoupler-stimulated respiration. The accumulated Ba2+ decreases the measured Vmax of K+ influx, while having little effect on the apparent Km for K+. The inhibition of K+ influx by Ba2+ is seen in the presence and absence of mersalyl, an activator of K+ influx. In contrast, under the conditions studied, Ba2+ has no apparent effect on the rate of unidirectional K+ efflux. These data are consistent with the idea that K+ may enter and leave mitochondria via separate mechanisms.

Y 3+ , La 3+ , and some bivalent metals inhibited the opening of the Tl + -induced permeability transition pore in Ca 2+ -loaded rat liver mitochondria

“…RR inhibited the Ca 2+ uptake in a noncompetitive manner [11,19,[23][24][25]. On the other hand, bivalent cations such as Sr 2+ [22,26], Ba 2+ [13,27], Mn 2+ [14,22,28], Cd 2+ [22,29], and Ni 2+ [17,30] as well as Y 3+ [31], La 3+ [18,22,24,25,32], Nd 3+ [25], Pr 3+ and Ga 3+ [9,31,33,34] are competitive inhibitors of the Ca 2+ uptake, which occurs with the participation of low-affinity sites [9], facing the cytoplasmic side of IMM. Maximal inhibition of the uptake was shown in experiments with La 3+ and RR [19,20,22] which resulted in their interaction with these sites [25].…”