Babesia bovis: Immunity induced by vaccination with a lipid enriched fraction

Goodger, B. V.; Commins, M.A.; Waltisbuhl, D.J.; Wright, I.G.; Rode-Bramanis, K.

doi:10.1016/0020-7519(90)90129-b

Cited by 11 publications

(9 citation statements)

References 14 publications

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“…However, patients infected with Babesia display lipid abnormalities and markedly low HDL levels, suggesting these parasites depend on host lipid metabolism as well ( Cunha et al, 2000 ; Bock et al, 2017 ; Table 1 ). Similar abnormalities have been observed in Babesia -infected cattle and canines ( Goodger et al, 1990 ; Milanović et al, 2019 ). These parasites have been demonstrated to rewire lipid biosynthesis to produce PC during the blood stage, much like Plasmodium ( Florin-Christensen et al, 2000 ).…”

Section: Intracellular Growthsupporting

confidence: 81%

Lipid hijacking: A unifying theme in vector-borne diseases

O’Neal

Butler

Rolandelli

et al. 2020

eLife

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Vector-borne illnesses comprise a significant portion of human maladies, representing 17% of global infections. Transmission of vector-borne pathogens to mammals primarily occurs by hematophagous arthropods. It is speculated that blood may provide a unique environment that aids in the replication and pathogenesis of these microbes. Lipids and their derivatives are one component enriched in blood and are essential for microbial survival. For instance, the malarial parasite Plasmodium falciparum and the Lyme disease spirochete Borrelia burgdorferi, among others, have been shown to scavenge and manipulate host lipids for structural support, metabolism, replication, immune evasion, and disease severity. In this Review, we will explore the importance of lipid hijacking for the growth and persistence of these microbes in both mammalian hosts and arthropod vectors.

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Section: Intracellular Growthsupporting

confidence: 81%

Lipid hijacking: A unifying theme in vector-borne diseases

O’Neal

Butler

Rolandelli

et al. 2020

eLife

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“…1985). The inducement of partial protection has also been reported by Goodger et al . (1990), who used four proteins of molecular weights ranging from 12 to 18 kDa.…”

Section: Discussionsupporting

confidence: 74%

“…Other proteins identified with molecular weights of 22 and 70 kDa confer partial protection against isolated proteins of virulent samples, and have been considered important candidates for immunization (Commins et al, 1985). The inducement of partial protection has also been reported by Goodger et al (1990), who used four proteins of molecular weights ranging from 12 to 18 kDa. It is possible that the proteins of 19 kDa (clones 17 and 20), 20 kDa (clone 15) and 73 kDa (clones 9, 13, 15, 20, 22, 23, 29, 34 and 36) identified in this study refer to the same proteins.…”

Section: Discussionmentioning

confidence: 84%

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Production and Characterization of a Panel of Monoclonal Antibodies for the Identification of Antigens of Babesia bovis

Figueiredo

Martins²,

Ribeiro³

et al. 2000

Journal of Veterinary Medicine, Series B

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A panel of monoclonal antibodies was produced and characterized by an indirect fluorescent antibody test (IFAT), an enzyme-linked immunosorbent assay (ELISA) and Western blotting with the aim of identifying antigens of Babesia bovis. After fusion, the resultant hybrids were selected by the IFAT, cloned, maintained in culture in vitro, and cryopreserved in liquid nitrogen. Ten clones producing monoclonal antibodies were found to react against the entire merozoites, three reacted on the surface of the merozoites, and one clone reacted against the polar region of the merozoites. All monoclonal antibodies reacted in ELISA, with the optical density varying from 0.368 to 0.502 (cut off = 0.022). The bands recognized by the monoclonal antibodies in Western blotting had molecular weights ranging from 162 to 19 kDa. Four clones recognized a single band of 73 kDa, and another four did not react in Western blotting.

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“…Considering that B. bovis lipids are relevant antigens (Goodger et al 1990; Shoda et al 2000) that can be recognized by innate immune receptors such as TLR2 (Gimenez et al 2010), we here analysed the production of NO, a macrophage secretory product with inhibitory effect in in vitro growth of intracellular B. bovis (Montealegre et al 1985; Shoda et al 2000). First, we assessed the induction of NO in bovine macrophages stimulated with the lipid extracts of B. bovis merozoites from attenuated R1A (L A ) or virulent S2P (L V ) strains, in the presence of IFN γ .…”

Section: Resultsmentioning

confidence: 99%

Babesia bovis:lipids from virulent S2P and attenuated R1A strains trigger differential signalling and inflammatory responses in bovine macrophages

et al. 2013

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The intra-erythrocytic protozoan Babesia bovis is an economically important pathogen that causes an acute and often fatal infection in adult cattle. Babesiosis limitation depends on the early activation of macrophages, essential cells of the host innate immunity, which can generate an inflammatory response mediated by cytokines and nitric oxide (NO). Herein, we demonstrate in bovine macrophages that lipids from B. bovis attenuated R1A strain (LA) produced a stronger NO release, an early TNFα mRNA induction and 2-fold higher IL-12p35 mRNA levels compared to the lipids of virulent S2P strain (LV). Neither LA nor LV induced anti-inflammatory IL-10. Regarding signalling pathways, we here report that LA induced a significant phosphorylation of p38 and extracellular signal-regulated kinases 1 and 2 (ERK1/2) whereas LV only induced a reduced activation of ERK1/2. Besides, NF-κB was activated by LA and LV, but LA produced an early degradation of the inhibitor IκB. Interestingly, LV and the majority of its lipid fractions, exerted a significant inhibition of concanavalin A-induced peripheral blood mononuclear cell proliferation with respect to LA and its corresponding lipid fractions. In addition, we determined that animals infected with R1A developed a higher increase in IgM anti-phosphatidylcholine than those inoculated with S2P. Collectively, S2P lipids generated a decreased inflammatory response contributing to the evasion of innate immunity. Moreover, since R1A lipids induced a pro-inflammatory profile, we propose these molecules as good candidates for immunoprophylactic strategies against babesiosis.

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Babesia bovis: Immunity induced by vaccination with a lipid enriched fraction

Cited by 11 publications

References 14 publications

Lipid hijacking: A unifying theme in vector-borne diseases

Lipid hijacking: A unifying theme in vector-borne diseases

Production and Characterization of a Panel of Monoclonal Antibodies for the Identification of Antigens of Babesia bovis

Babesia bovis:lipids from virulent S2P and attenuated R1A strains trigger differential signalling and inflammatory responses in bovine macrophages

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