Bacillus cereus liquid fertilizer was produced from Agaricus bisporus industrial wastewater

Huang, Jinhong; Zhuo, Yizhen; Jin-qiu, LU; Lai, Qingyu; Zhang, Yawen

doi:10.1016/j.jbiotec.2021.01.001

Cited by 14 publications

(2 citation statements)

References 50 publications

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“…The low and high levels of each variable were denoted by (−1) and (+1), respectively. The high level is the initially basic value of each factor in the cell-free reaction, which is 1.25 times higher than the corresponding low level. , Note that the initial reaction temperature of 30 °C was set as a low level. The levels of each variable selected for our experimental design are shown in Table .…”

Section: Materials and Methodsmentioning

confidence: 99%

Combining Cell-Free Expression and Multifactor Optimization for Enhanced Biosynthesis of Cinnamyl Alcohol

Zhang

Liu

Ling

et al. 2023

J. Agric. Food Chem.

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Cell-free expression systems have emerged as a potent and promising platform for the biosynthesis of chemicals by reconstituting in vitro expressed enzymes. Here, we report cell-free biosynthesis of cinnamyl alcohol (cinOH) with enhanced productivity by using the Plackett−Burman experimental design for multifactor optimization. Initially, four enzymes were individually expressed in vitro and directly mixed to reconstitute a biosynthetic route for the synthesis of cinOH. Then, the Plackett−Burman experimental design was used to screen multiple reaction factors and found three crucial parameters (i.e., reaction temperature, reaction volume, and carboxylic acid reductase) for the cinOH production. With the optimum reaction conditions, approximately 300 μM of cinOH was synthesized after 10 h of cell-free biosynthesis. Extending the production time to 24 h also increased the production to a maximum yield of 807 μM, which is nearly 10 times higher than the initial yield without optimization. This study demonstrates that cell-free biosynthesis can be combined with other powerful optimization methodologies such as the Plackett−Burman experimental design for enhanced production of valuable chemicals.

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Section: Materials and Methodsmentioning

confidence: 99%

Combining Cell-Free Expression and Multifactor Optimization for Enhanced Biosynthesis of Cinnamyl Alcohol

Zhang

Liu

Ling

et al. 2023

J. Agric. Food Chem.

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“…The Plackett–Burman statistical design is an efficient method for screening and identifying the significant parameters among a large number of variables [ 50 ]. The main factors were screened based on seven factors (carbon source, nitrogen source, mineral salt, initial pH, inoculation amount, fermentation temperature and fermentation time), and the contents of andrimid (Y) were taken as the response value.…”

Section: Methodsmentioning

confidence: 99%

Improved production of andrimid in Erwinia persicina BST187 strain by fermentation optimization

Cheng,

Ge,

Zhao

et al. 2023

BMC Microbiol

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Background Andrimid is reported to be a novel kind of polyketide-nonribosomal peptide hybrid product (PK-NRPs) that inhibits fatty acid biosynthesis in bacteria. Considering its great potential in biomedicine and biofarming, intensive studies have been conducted to increase the production of andrimid to overcome the excessive costs of chemosynthesis. In screening for species with broad-spectrum antibacterial activity, we detected andrimid in the fermentation products of Erwinia persicina BST187. To increase andrimid production, the BST187 fermentation medium formulation and fermentation conditions were optimized by using systematic design of experiments (One-Factor-At-A-Time, Plackett–Burman design, Response Surface Methodology). Results The results indicate that the actual andrimid production reached 140.3 ± 1.28 mg/L under the optimized conditions (trisodium citrate dihydrate-30 g/L, beef extract-17.1 g/L, MgCl2·6H2O-100 mM, inoculation amount-1%, initial pH-7.0, fermentation time-36 h, temperature-19.7℃), which is 20-fold greater than the initial condition without optimization (7.00 ± 0.40 mg/L), consistent with the improved antibacterial effect of the fermentation supernatant. Conclusions The present study provides valuable information for improving andrimid production via optimization of the fermentation process, which will be of great value in the future industrialization of andrimid production.

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Isolation and characterization of Bacillus cereus virulent phage CA1

Xiao,

Hu,

Chen

et al. 2024

Aquaculture

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Bacillus cereus liquid fertilizer was produced from Agaricus bisporus industrial wastewater

Cited by 14 publications

References 50 publications

Combining Cell-Free Expression and Multifactor Optimization for Enhanced Biosynthesis of Cinnamyl Alcohol

Combining Cell-Free Expression and Multifactor Optimization for Enhanced Biosynthesis of Cinnamyl Alcohol

Improved production of andrimid in Erwinia persicina BST187 strain by fermentation optimization

Isolation and characterization of Bacillus cereus virulent phage CA1

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