Bacillus marinisedimentorum sp. nov., isolated from marine sediment

Abstract

A novel Gram-stain-positive, motile and facultatively anaerobic strain, designated NC2-31, was isolated from sediment from the coast of Weihai, PR China. Optimal growth occurred at 37 °C, pH 7.5 and with 2.0-3.0 % (w/v) NaCl. MK-7 was the major respiratory quinone. Meso-diaminopimelic acid was a diagnostic diamino acid in the peptidoglycan. The major polar lipids of NC2-31 were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG) and phosphatidylethanolamine (PE). The genomic DNA G+C content of the strain was 46.3 mol%. The predominant cellular fatty acids (>10.0 %) of NC2-31 were iso-C15 : 0 (18.9 %), anteiso-C15 : 0 (15.8 %), summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH) (15.3 %) and iso-C16 : 0 (10.3 %). Phylogenetic analysis based on 16S rRNA gene sequences revealed that NC2-31 should be classified as representing a member of the genus Bacillus. Based on data from the current polyphasic study, NC2-31 represents a novel species within the genus Bacillus, for which the name Bacillusmarinisedimentorum sp. nov. is proposed with type strain NC2-31 (=KCTC 33721=MCCC 1K01239).

Bacillus salinus sp. nov., isolated from commercial solar salt

“…At the time of writing, 379 species in the genus Bacillus (www.bacterio.net/bacillus.html) have been isolated from various sources, such as air, soil, freshwater sediment, marine, plants and animals [3–7]. A total of 32 marine species of the genus Bacillus have been isolated from sites such as seawater, marine sediment, sea anemone, marine hydrothermal vent and solar saltern [8–13].…”

Litoribacterium kuwaitense gen. nov., sp. nov., isolated from a Kuwait tidal flat

“…halotolerans S1203 T was isolated from marine sediment at a water depth of almost 865 m in the Okinawa Trough [38] while B. marinisedimentorum was isolated from a coastal sediment sample from Weihai, PR China [55]. On the other hand, strain SIJ1 T was isolated from an Arabian Gulf’s tidal flat, an environment known for its extreme high fluctuations in temperature and high salinity level due to a low precipitation (low rainwater fall) and high evaporation rates [10].…”

“…Upon comparison to A. halotolerans S1203 T and B. marinisedimentorum KCTC 33721 T genomes using RAST annotation, the SIJ1 T genome was the only one that contained cold shock and heat shock coding genes, and in total, it included 114 coding genes within the six subcategories of the stress-resistant subsystem while A. halotolerans S1203 T contained 49 and B. marinisedimentorum KCTC 33721 T contained 57 genes. A. halotolerans S1203 T was isolated from marine sediment at a water depth of almost 865 m in the Okinawa Trough [ 38 ] while B. marinisedimentorum was isolated from a coastal sediment sample from Weihai, PR China [ 55 ]. On the other hand, strain SIJ1 T was isolated from an Arabian Gulf’s tidal flat, an environment known for its extreme high fluctuations in temperature and high salinity level due to a low precipitation (low rainwater fall) and high evaporation rates [ 10 ].…”