Bacillus pumilus Cyanide Dihydratase Mutants with Higher Catalytic Activity

Crum, M.A.; Sewell, B. Trevor; Benedik, Michael J.

doi:10.3389/fmicb.2016.01264

Cited by 19 publications

(31 citation statements)

References 22 publications

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“…Meanwhile, stability changes when explaining cyanide complexes since these substances have a wide range of chemical and biological characteristics (DASH;GAUR;BALOMAJUMDER, 2009). Some cyanide-metal complexes can even be stable at pH 7 such as the Ni-CN complex where free cyanide would be highly volatile (SNOEYINK;JENKINS, 1980).…”

Section: Cyanide Stabilitymentioning

confidence: 99%

“…Cyanide-containing wastes and wastewaters can be treated using natural degradation, chemical or biological processes (DASH;GAUR;BALOMAJUMDER, 2009). Currently, chemical processes such as alkaline chlorination, oxidation with hydrogen peroxide, and INCO SO2/Air process are the most widely used.…”

Section: Introductionmentioning

confidence: 99%

“…Currently, chemical processes such as alkaline chlorination, oxidation with hydrogen peroxide, and INCO SO2/Air process are the most widely used. However, these processes can generate other pollutants, use expensive reagents, require complex facilities or do not get to treat some cyanide compounds (BOTZ, 1999;DASH;GAUR;BALOMAJUMDER, 2009;KUYUCAK;AKCIL, 2013).…”

Section: Introductionmentioning

confidence: 99%

“…In this context, microbial degradation of cyanide has appeared as a cheap, simple and viable alternative technology for cyanide treatment (DASH;GAUR;BALOMAJUMDER, 2009). During the biological treatment of cyanide, it is consumed within the metabolic pathways of microorganisms transforming it into ammonia, formate, formide, and other non-toxic products (GUPTA;…”

Section: Introductionmentioning

confidence: 99%

“…Several authors have previously reported the use of microbial strains to degrade cyanide (ANNACHHATRE;AMORNKAEW, 2000;BAXTER;CUMMINGS, 2006;DASH;GAUR;BALOMAJUMDER, 2009;KUYUCAK;AKCIL, 2013). This treatment can be performed with individual strains or in consortia (MEKUTO et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

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Biological degradation of cyanide using native bacteria isolated from a cassava-processing effluent.

Arévalo¹,

Tania²

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Cyanide (CN) can be present in the environment due to human activities including gold mining, electroplating, and nylon production. During the industrial processing of cassava, cyanide is liberated in effluents due to the presence of cyanogenic glycosides in the pulp. Cyanide is toxic for most living organisms since it inhibits the action of the cytochrome c oxidase and stops the electron transport chain during cellular respiration. Because of this, cyanide-containing effluents need to be treated prior to discharge to the environment, which can be achieved by chemical, physical or biological methods. In this context, this work evaluates the cyanide-degradation capacity of native bacteria isolated from a cassavaprocessing effluent and the best conditions for the biological removal of cyanide. In total, sixteen cyanide-resistant bacteria were isolated, identified by mass spectrometry technique MALDI-TOF, and tested individually for their ability to degrade cyanide. Eight different species were identified among the isolated strains. Four of them, belonging to the Klebsiella oxytoca, Bacillus pumilus, Corynebacterium glutamicum, and Serratia marcescens species, were selected for further assays to assess their susceptibility to pH and cyanide initial concentration. Results showed that cyanide degradation was inhibited when pH was above 9 and that higher initial cyanide concentrations resulted in lower cyanide degradation percentage. However, strains could still keep their degradation capacity even in high initial cyanide concentrations of 1000mg.L-1. Isolated Klebsiella oxytoca and Serratia marcescens were thus selected for optimization of the degradation process by analyzing the influence of carbon source, temperature and rotation speed. Both strains showed greater degradation rates when using sodium acetate as a carbon source and incubated at 30°C. Agitation speed did not show an effect on cyanide degradation by Klebsiella oxytoca, but enhanced degradation by Serratia marcescens strain. Under optimal incubation conditions, isolated Klebsiella oxytoca and Serratia marcescens strains could degrade cyanide from a synthetic 150mg.L-1 freecyanide solution, pH 8, using sodium acetate as a carbon source by 43 and 41% after 24hour of incubation, respectively, showing their potential to be used in the biodegradation of cyanide-containing effluents.

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Section: Cyanide Stabilitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Biological degradation of cyanide using native bacteria isolated from a cassava-processing effluent.

Arévalo¹,

Tania²

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Enzyme engineering and its industrial applications

Amatto

Rosa-Garzon

Simões

et al. 2021

Biotech and App Biochem

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Recently, there has been an increase in the demand for enzymes with modified activity, specificity, and stability. Enzyme engineering is an important tool to meet the demand for enzymes adjusted to different industrial processes. Knowledge of the structure and function of enzymes guides the choice of the best strategy for engineering enzymes. Each enzyme engineering strategy, such as rational design, directed evolution, and semi-rational design, has specific applications, as well as limitations, which must be considered when choosing a suitable strategy. Engineered enzymes can be optimized for different industrial applications by choosing the appropriate strategy. This review features engineered enzymes that have been applied in food, animal feed, pharmaceuticals, medical applications, bioremediation, biofuels, and detergents.

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Enzyme engineering: Reshaping the biocatalytic functions

Ali

Ishqi

Husain

2020

Biotech & Bioengineering

124

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Enzyme engineering is a powerful tool to fine‐tune the enzymes. It is a technique by which the stability, activity, and specificity of the enzymes can be altered. The characteristic properties of an enzyme can be amended by immobilization and protein engineering. Among them, protein engineering is the most promising, as in addition to amending the stability and activity, it is the only way to modulate the specificity and stereoselectivity of enzymes. The current review sheds light on protein engineering and the approaches applied for it on the basis of the degree of knowledge of structure and function of enzymes. Enzymes, which have been engineered are also discussed in detail and categorized on the basis of their respective applications. This will give a better insight into the revolutionary changes brought by protein engineering of enzymes in various industrial and environmental processes.

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Bacillus pumilus Cyanide Dihydratase Mutants with Higher Catalytic Activity

Cited by 19 publications

References 22 publications

Biological degradation of cyanide using native bacteria isolated from a cassava-processing effluent.

Biological degradation of cyanide using native bacteria isolated from a cassava-processing effluent.

Enzyme engineering and its industrial applications

Enzyme engineering: Reshaping the biocatalytic functions

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