Bacillus pumilus strain YSPMK11 as plant growth promoter and bicontrol agent against Sclerotinia sclerotiorum

Abstract

A study was executed in a direction to attenuate Sclerotinia stalk rot (SSR) disease through biocontrol agent and also to enhance crop productivity. Culture filtrate of bacterial strain YSPMK11 inhibited growth of Sclerotinia sclerotiorum in vitro which also exhibited higher plant growth promoting attributes. Interaction studies revealed maximum (81.50%) growth inhibition at 35°C and pH 7.0 after 72 h incubation period with 15% culture filtrate. Based upon 16S rRNA gene sequence strain, YSPMK11 was identified as Bacillus pumilus. Furthermore, the genome of this isolate was searched for antimicrobial lipopeptide, i.e., ItuD and SrfC genes. The PCR amplification results showed the presence of both these lipopeptide genes in isolate YSPMK11. Iturin A as antifungal compound was identified as major components of fraction through GC/MS. In field experiments, the application of strain YSPMK11 cell suspension (10 8 CFU/ml) suppressed disease severity by 93% and increased curd yield by 36% which was more that of commercially used fungicide in farmer practices under mid-hills of Himachal Pradesh.Conclusively, our study is first to demonstrate the effect of B. pumilus strain YSPMK11 in suppression of SSR under field conditions and would be employed as an efficient biocontrol agent to replace commercial fungicides in cauliflower cropping system. In addition, the presence of both lipopeptide genes (ItuD and SrfC) and iturin A in this isolate makes him potent strain for biological control application in agriculture.

Sugarcane growth and nutrition levels are differentially affected by the application of PGPR and cane waste

“…Therefore, it is difficult for plants to absorb it directly. Solubilization and mineralization of phosphorus by phosphate solubilizing bacteria such as B. pumilus and B. subtilis has been reported by, Kaushal, Kumar, and Kaushal, (2017), and this study shows that it is an important state that can be achieved. Filter cake compost has been shown to be an effective type of fertilization that promotes phosphate (PO 4 ) solubilization and increases P availability in the soil compared to other types of fertilization.…”

Metagenomic Insights of the Root Colonizing Microbiome Associated with Symptomatic and Non-Symptomatic Bananas in Fusarium Wilt Infected Fields

“…The endophytic microbiome corresponds to the microbes that colonize and reside within the plant tissues [2]. These microbiomes have been exploited as biocontrol agents promoting plant growth and health in various crops [3,4]. Despite this, endophytic microbiota are poorly studied.…”

POLYPHASIC CHARACTERIZATION OF Bacillus STRAINS ISOLATED FROM MAIZE

“…These features allow for the occupation of several agroecosystems and are favorable in the development of bioinoculants (Bahadir et al, 2018). In the present study, the isolate B32 was identified as B. pumilus, a species that has been reported as aerobic, as well as having antifungal activity (Kaushal et al, 2017;Cruz et al, 2018). On the other hand, the isolate B116 was identified as B. thuringiensis, a bacterium that normally produces insecticidal crystal proteins against several insects, nematodes, protozoa and mites, being the species most widely used as biopesticide (Chattopadhyay et al, 2004;Sanahuja et al, 2011).…”